FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987892
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29790.30155118170812195No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27330.2766496742558903No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24810.2511408129633603No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG24320.24618075660092398No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23880.24172682843873625No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23600.238892510517344No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG22140.2241135670700846No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19630.19870593141760434No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19580.19819980321735572No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18580.18807723921238353No Hit
CCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT18390.18615395205143884No Hit
CTGTTAAGGTTCATCAAAAAACGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGTTGATGTGC17810.18028286492855494No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17540.17754977264721244No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17370.17582893676636718No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16950.17157745988427886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16550.16752843428228997No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16290.1648965676409972No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16150.16347940868030109No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15790.1598352856385111No Hit
GGGTCTCTCTCCCAGCTTGTTCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC15630.15821567539771553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14710.1489029165131411No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT14280.14455021399100307No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14190.14363918323055555No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13950.14120976786936223No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT13910.14080486530916336No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG13890.1406024140290639No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13630.13797054738777112No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13620.1378693217477214No Hit
CCTGCACTCTGAGCAGTGGGCACATCAACTACGCCATCGCATGGCATCAG13500.13665461406712473No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13120.1328080397452353No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATAATGAGGTAGC13090.13250436282508615No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13070.13230191154498672No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12970.13128965514448948No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTTCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC12700.12855656286314698No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12360.12511489110145643No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12220.12369773214076032No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG12110.12258425010021338No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11990.12136954241961673No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTTCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT11930.12076218857931839No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11920.12066096293926866No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11520.11661193733727979No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11370.11509355273653396No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAG11080.11215800917509201No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11020.11155065533479368No Hit
CACCTGCACTCTGAGCAGTGGGCACATCAACTACGCCATCGCATGGCATC11000.11134820405469424No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11000.11134820405469424No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10990.11124697841464451No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10920.11053839893429647No Hit
GCTCTGGGGCTGAGCGCTACCTCATTATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC10870.11003227073404784No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10560.10689427589250647No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10410.10537589129176064No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC10320.10446486053131314No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC10300.10426240925121369No Hit
GCACAGGGTCTCTCTCCCAGCTTGTTCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG10280.10405995797111425No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA10280.10405995797111425No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC10240.10365505541091535No Hit
TCCTCAGACTGGAGGCTGGAGATAATGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC10200.10325015285071648No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10190.10314892721066675No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10120.1024403477303187No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10050.10173176824997066No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9920.10041583492932425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG300.002457385668.26144470
GGTATCA14300.044.6803971
GGATGGG5900.035.86618470
GTATCAA31450.034.504491
TCTGGAG5950.032.12303570
GTGGTAT4150.030.9547121
TCCGAGA4050.030.33842570
TACGGTA450.005113094630.0404744
TATCAAC35800.030.022542
ATCAACG37000.028.9545543
TGTGTCG16800.028.84858970
TGGTATC4050.028.3744322
TCAACGC37750.028.2897724
CAACGCA38050.028.0638835
AACGCAG38950.027.6813036
AGAGTAC40450.026.92183111
CGCAGAG40200.026.7473228
CAGAGTA40600.026.73635710
GACAGGT1900.026.6887931
TAGATTG908.111112E-626.2827555
Produced by FastQC (version 0.11.5)