FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047264
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52510.5014017477923427No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38160.36437803648363737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36390.34747685397378314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35610.340028875240627No Hit
GTAGGGGACAGAGTCGTCATCACTTGCCAGGCGAGTCAGGACGTTAGCAACCGTGTAAATTGGTATCAGCAAAAA33260.3175894521343234No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31080.29677330644422034No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29400.2807315060958841No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29000.27691202982247076No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25480.2433006386164329No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24750.23633009441745345No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23900.22821370733645002No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23280.22229351911265927No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22250.2124583677086198No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22020.2102621688514071No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21500.2052968496959697No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21020.20071347816787363No Hit
GTTAGCAACCGTGTAAATTGGTATCAGCAAAAACCAGGGCAAGCCCCTAA20720.1978488709628136No Hit
GGTAAATTCTGTCCCAGATCCACTCCCAATGAATCTAAATGGGACCCCTGATTCCAAAGCGGATGCATCGTAGAT20700.19765789714914292No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20660.19727594952180155No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC20360.19441134231674154No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20070.19164222201851683No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19680.18791823265193874No Hit
GTATGATAATGTCCCATATGCTTTTGGCCAAGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT19490.1861039814220674No Hit
CCCCTAAGCTCCTGATCTACGATGCATCCGCTTTGGAATCAGGGGTCCCA19350.18476716472637272No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18980.18123414917346534No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18910.180565740825618No Hit
GAATTTACCTTCACCATCAGTAGGGTGCAGCCTGAAGATATTGCAACATATTTCTGTCAAGAGTATGATAATGTC18890.18037476701194732No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCTTGGCCAAAAGCA18730.17884697650258197No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18020.1720674061172732No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17920.17111253704891985No Hit
GTAATATACAGCCGTGTCGTCGTCTCTCAGGCTGTCCATTTGGAGATACA17580.16786598221651847No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17010.16242322852690438No Hit
TTATCATACTCTTGACAGAAATATGTTGCAATATCTTCAGGCTGCACCCTACTGATGGTGAAGGTAAATTCTGTC16960.1619457939927277No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16910.16146835945855104No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16610.15860375225349102No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAAATGTGACAT16020.15297002475020627No Hit
CTGTAGGGGACAGAGTCGTCATCACTTGCCAGGCGAGTCAGGACGTTAGC15780.1506783389861582No Hit
GTCTCCAGGCTCCCTGTCTGCCTCTGTAGGGGACAGAGTCGTCATCACTTGCCAGGCGAGTCAGGACGTTAGCAA15770.1505828520793229No Hit
GAGTATGATAATGTCCCATATGCTTTTGGCCAAGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT15470.14771824487426283No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15310.14619045436489747No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14890.14218000427781344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14270.13625981605402268No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14260.13616432914718735No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14190.13549592079934No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14130.134922999358328No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14000.13368166956946864No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13940.13310874812845663No Hit
GTTGCTAACGTCCTGACTCGCCTGGCAAGTGATGACGACTCTGTCCCCTACAGAGGCAGACAGGGAGCCTGGAGA13580.12967121948238458No Hit
AGTCAGGACGTTAGCAACCGTGTAAATTGGTATCAGCAAAAACCAGGGCA13530.1291937849482079No Hit
GGACAGAGTCGTCATCACTTGCCAGGCGAGTCAGGACGTTAGCAACCGTG13450.12842988969352523No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13420.12814342897301922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13030.12441943960644117No Hit
GTCCCCTACAGAGGCAGACAGGGAGCCTGGAGACTGAGTCATCTGGATGTCACATTTGGCACCTGAGAGCCAGAG12990.12403749197909981No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12960.12375103125859382No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12780.1220322669355578No Hit
GATCTACGATGCATCCGCTTTGGAATCAGGGGTCCCATTTAGATTCATTGGGAGTGGATCTGGGACAGAATTTAC12700.12126837168087512No Hit
GCCTGAAGATATTGCAACATATTTCTGTCAAGAGTATGATAATGTCCCAT12670.12098191096036912No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12590.12021801570568644No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12530.11964509426467443No Hit
ATCATACTCTTGACAGAAATATGTTGCAATATCTTCAGGCTGCACCCTAC12500.11935863354416841No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12500.11935863354416841No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAAATGTGACATCCAGATGACTCAGTCTCCAGGCTCC12470.11907217282366242No Hit
GCTTAGGGGCTTGCCCTGGTTTTTGCTGATACCAATTTACACGGTTGCTA12410.11849925138265041No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12400.11840376447581508No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12370.11811730375530906No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAAATGTGACATCCAGATGACTCAGTCTCCAGG12330.11773535612796773No Hit
GTATATTACTGTTCGAGAGGGCGTCATGAGTGGAACTTCAGGGACTTCGC12280.11725792159379106No Hit
GTCCCAGATCCACTCCCAATGAATCTAAATGGGACCCCTGATTCCAAAGC12010.114679775109237No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11920.113820392947719No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11790.11257906315885965No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11610.11086029883582364No Hit
CCTCTGTAGGGGACAGAGTCGTCATCACTTGCCAGGCGAGTCAGGACGTT11450.10933250832645827No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11250.10742277018975159No Hit
CTACTGATGGTGAAGGTAAATTCTGTCCCAGATCCACTCCCAATGAATCT11180.10675436184190423No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11070.10570400586671555No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10970.1047491367983622No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10960.10465364989152687No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10910.1041762153573502No Hit
CCCTACTGATGGTGAAGGTAAATTCTGTCCCAGATCCACTCCCAATGAATCTAAATGGGACCCCTGATTCCAAAG10830.10341232010266752No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10760.10274391175482019No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10730.10245745103431418No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10720.10236196412747883No Hit
GTATACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10600.10121612124545483No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10480.10007027836343081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT555.362899E-447.54083670
GTATATA405.442248E-542.512421
GCTTGTA751.0022632E-940.8119241
GTATCAA24100.036.9734571
CACGTCG400.00238630935.09810651
CGGGTAT400.00277641234.0310718
TATCAAC27050.032.9270742
GTGCTAG1700.032.009351
CGCTATT450.00376738131.97547563
ATCAACG27850.031.6120193
TCAACGC28100.031.3307724
CAACGCA28350.031.17295
AACGCAG28600.031.0296276
GTGTATA553.5417877E-430.9181231
CTTGTAT553.550724E-430.9048352
TACGTAC1550.030.7259912
AATACGT1550.030.72452210
AATGGTA4950.030.37331470
ATACGTA1600.029.76580411
AATCGTC6200.029.59020863
Produced by FastQC (version 0.11.5)