FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858262
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46830.545637579200757No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGTGGCGCTGGTTGTATATGCGCTGCAGTAATAAT46100.537132017961881No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42160.4912252901794557No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGTGGCGCTGGTTGTATATGCGCTG41070.47852520558990147No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG35850.41770461700506367No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32450.378089674248656No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCGGCAGTGACCTT31000.3611950663084233No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30020.3497766416315764No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC29440.3430187984554833No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28960.3374261006545787No Hit
TATACAACCAGCGCCACTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC28440.3313673447035987No Hit
CTCCTGCACTGGAACCGGCAGTGACCTTGGTGGTTTTCAGTCTGTCTCCTGGTATCAACACCTCCCAGGCAAAGC28020.3264737341278071No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC25970.3025882539364437No Hit
GATTATTACTGCAGCGCATATACAACCAGCGCCACTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT25580.29804418697320867No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25360.29548086714779404No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24770.2886065094341821No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCGCATAT24690.2876743931340313No Hit
GGTTGTATATGCGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC24140.28126609357049476No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23630.27532385215703364No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23030.2683329799059028No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22800.2656531455429694No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22710.2646045147052998No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22430.26134210765477206No Hit
GTCGATCACCATCTCCTGCACTGGAACCGGCAGTGACCTTGGTGGTTTTCAGTCTGTCTCCTGGTATCAACACCT22360.26052650589214016No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21590.251554886503189No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21000.24468052878957708No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGTGGCGCTGG20550.23943737460122902No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20350.23710708385085208No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20320.23675754023829554No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19980.23279604596265477No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19790.23058226974979668No Hit
GTATCAACACCTCCCAGGCAAAGCCCCCAAACTCATGATTCATGGTGTCA19770.23034924067475898No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19720.22976666798716475No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT18610.2168335543225728No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18590.21660052524753512No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18420.21461977810971472No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18420.21461977810971472No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17780.20716284770850862No Hit
GTCTCCTGGTATCAACACCTCCCAGGCAAAGCCCCCAAACTCATGATTCA17400.20273529528279244No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG16870.19656002479429358No Hit
GTGTTGATACCAGGAGACAGACTGAAAACCACCAAGGTCACTGCCGGTTCCAGTGCAGGAGATGGTGATCGA16830.1960939666442182No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16500.1922489869060963No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15620.1819957076044378No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC15280.17803421332879704No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15170.17675255341608972No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15070.17558740804090128No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15030.1751213498908259No Hit
GTTTGGTCCCTCCGCCGAACACCCAAGTGGCGCTGGTTGTATATGCGCTGCAGTAATAATCAGCCTCGTCCTCAG14400.16778093402713856No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14400.16778093402713856No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14280.1663827595769124No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14040.1635864106764601No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14010.16323686706390356No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13990.16300383798886586No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13950.1625377798387905No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13860.16148914900112088No Hit
CCCAAGTGGCGCTGGTTGTATATGCGCTGCAGTAATAATCAGCCTCGTCC13800.1607900617760078No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13770.16044051816345126No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13560.15799371287555547No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13230.15414873313743355No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13180.1535661604498393No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC13110.1527505586872074No Hit
GACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTAGTGAC13050.15205147146209433No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12850.1497211807117174No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCC12710.14808997718645356No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12480.1454101428235201No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12110.14109910493532277No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11920.1388853287224647No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG11920.1388853287224647No Hit
GTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAG11890.13853578510990816No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11880.1384192705723893No Hit
GGTATCAACACCTCCCAGGCAAAGCCCCCAAACTCATGATTCATGGTGTC11690.13620549435953125No Hit
GTATATGCGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA11620.13538989259689932No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11560.13469080537178624No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11420.1330596018465224No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGTGGCG11340.13212748554637163No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11330.13201097100885278No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11030.1285155348832874No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10990.12804947673321201No Hit
CCAGGAGACAGACTGAAAACCACCAAGGTCACTGCCGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGA10950.12758341858313663No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT10910.12711736043306124No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10730.125020098757722No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10640.12397146792005238No Hit
CCACTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC10440.12164117716967546No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCG10420.12140814809463776No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10410.12129163355711892No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCC10400.12117511901960007No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACC10370.12082557540704353No Hit
CCCCTGAGGGCCGATTAGTGACACCATGAATCATGAGTTTGGGGGCTTTGCCTGGGAGGTGTTGATACCAGGAGA10320.1202430027194493No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC10290.11989345910689278No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC10150.11826225558162892No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10150.11826225558162892No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT10090.11756316835651584No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10080.117446653818997No Hit
GCGCATATACAACCAGCGCCACTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTG10040.1169805956689216No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT10000.11651453751884623No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9980.11628150844380854No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC9940.11581545029373315No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAGCGATT9930.1156989357562143No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9720.11325213046831854No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAGCGATTAGAAA9720.11325213046831854No Hit
CCTCCCAGGCAAAGCCCCCAAACTCATGATTCATGGTGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCT9650.11243652870568661No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9590.11173744148057353No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCGCATATACAACCA9500.11068881064290391No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9490.11057229610538506No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA9320.10859154896756468No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9290.10824200535500814No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTC9150.1066108018297443No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA8940.10416399654184852No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG8870.10334839477921662No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8850.10311536570417892No Hit
GTTTGGGGGCTTTGCCTGGGAGGTGTTGATACCAGGAGACAGACTGAAAA8800.10253279301658469No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT8800.10253279301658469No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8690.10125113310387737No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8650.10078507495380198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.5436969E-461.17391670
CCCGTTA200.006282110253.8610360
CGATTAA200.007363554651.74076541
AACGATT200.007431001451.6215939
GTGGTAT1650.041.148181
ATGGCAA2050.040.2852670
GTCGTAA350.001437374738.9147430
ATTGGGC350.001458969838.7968563
ACGAACC556.3998577E-638.4350752
AATCGTT557.5191674E-637.54297639
TTAATCG557.672845E-637.43243837
TACTCCG558.2583265E-637.033365
GTACGTA601.4402276E-436.03614869
GGTACGT551.6297182E-435.28719768
TCTGGAG4100.035.24960370
AACGAAC601.1807062E-535.15138651
CGTATGC501.8275894E-434.60651443
CGTTGAA601.3307466E-534.5429242
CTAAAGG2003.9108272E-1034.41032870
ACGAGAC808.1598046E-833.95318222
Produced by FastQC (version 0.11.5)