FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818470
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAGGTGCTGCTGGCCACCGCCTGACAGTAATAGT29540.36091732134348237No Hit
CCCCTGTGCTGGTCATCTTTCAAGACAGTAAGCGGCCCTCAGGGATCCCT29010.3544418243796352No Hit
GTATCCGTGGCCTCCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCAC28200.34454531015186873No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25610.3129009004606155No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25200.30789155375273375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24160.29518491820103365No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAACAAACATATTCATCC24020.293474409569074No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21310.26036384962185544No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21170.2586533409898958No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC21130.2581646242379073No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAGGTGCTGCTGGCCACCGCCTGA21000.2565762947939448No Hit
CCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA20830.2544992485979938No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGGGTGGCTGTGTTTCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC18840.23018559018656762No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17730.21662370031888767No Hit
CCTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC17420.21283614549097704No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17290.21124781604701456No Hit
CCCCAGGACAGACAGCCACCATCACCTGCTCTGGAGATAAGTTGGGGGAT16970.20733808203110682No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGGTGGCCAGCAGCACCT16660.2035505272031962No Hit
GACTATTACTGTCAGGCGGTGGCCAGCAGCACCTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT16510.20171783938323948No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16030.19585323835937785No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15720.19206568353146725No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCACCTGCTCTGG15570.1902329957115105No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15380.1879115911395653No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15300.18693415763558835No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15260.18644544088359988No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14930.18241352767969504No Hit
CTATTACTGTCAGGCGGTGGCCAGCAGCACCTATGTGGTCTTCGGCGGAG14680.17935904797976712No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14420.17618238909184208No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14140.1727613718279228No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13910.16995125050398915No Hit
CCACATAGGTGCTGCTGGCCACCGCCTGACAGTAATAGTCAGCCTCATCC13840.16909599618800933No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGGGTGG13770.1682407418720295No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCTTTCAAGACAGTAAGCGGCCC13470.16457536623211602No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13430.16408664948012755No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13320.16274267841215928No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12770.15602282307231788No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12660.15467885200434958No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12600.15394577687636687No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12330.15064693880044472No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11860.14490451696458026No Hit
GGCCAGTCCCCTGTGCTGGTCATCTTTCAAGACAGTAAGCGGCCCTCAGG11570.14136132051266387No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGGGT11560.14123914132466675No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11130.13598543624079074No Hit
CAGCCACCCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGGTGGCCAGCAGCA10860.1326865981648686No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10520.12853250577296663No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC10330.12621110120102141No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10280.12560020526103582No Hit
GGGTGGCTGTGTTTCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT10120.12364533825308197No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10120.12364533825308197No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10000.12217918799711656No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9870.12059085855315405No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGAAACACAG9860.12046867936515693No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9770.1193690666731829No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGGTCCACTGCAGGTAGG9560.11680330372524345No Hit
ATGTATTACTGTGCGAGACGTGGACACGTGGATATAGAGTCTGCCCGTAT9470.1157036910332694No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9400.11484843671728957No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGTATCCGTGGCCTCCTATGAGTTGACT9380.11460407834129535No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGTATCCGTGG9360.1143597199653011No Hit
GTGCTGGTCATCTTTCAAGACAGTAAGCGGCCCTCAGGGATCCCTGAGCG9340.11411536158930688No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9180.11216049458135302No Hit
GGACAGACAGCCACCATCACCTGCTCTGGAGATAAGTTGGGGGATGAATA9110.1113052402653732No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8980.10971691082141068No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8980.10971691082141068No Hit
GGAGATAAGTTGGGGGATGAATATGTTTGTTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTTT8950.10935037325741934No Hit
GACCAGCACAGGGGACTGGCCTGGCTTCTGCTGATACCAACAAACATATT8910.10886165650543086No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8870.10837293975344241No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8740.10678461030947989No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8690.1061737143694943No Hit
GATATAGAGTCTGCCCGTATATGGGACTTTCACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCACCATCC8680.10605153518149718No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8560.10458538492553178No Hit
GTCTTGAAAGATGACCAGCACAGGGGACTGGCCTGGCTTCTGCTGATACC8440.10311923466956639No Hit
GCACAGTATCCGTGGCCTCCTATGAGTTGACTCAGCCACCCTCAGTGTCC8330.1017752636015981No Hit
GGCCTGGCTTCTGCTGATACCAACAAACATATTCATCCCCCAACTTATCTCCAGAGCAGGTGATGGTGGCTGT8270.1010421884736154No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTG8230.10055347172162694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA200.003055405364.6141369
TACGTAT400.00867522149.6345370
AATGGTA4850.040.93569670
GTGGTAT1250.040.922941
GTATCAA13400.038.428881
TCGTATG753.9999577E-836.81906540
TCTCGTA709.914675E-734.41784338
ATCTCGT807.3228875E-834.37333737
TCTCCGA1103.274181E-1134.1045311
GTACTCA400.002747690834.102451
CGAGACT1155.4569682E-1132.63169523
CACGAGA1057.2577677E-1032.48249421
GCTAATC959.460564E-932.43837433
AATCTCG851.2601959E-732.31357236
CGTATGC751.6520862E-632.240641
TATCAAC16350.031.7038082
TAATCGT553.3020304E-431.2889538
ACGAGAC1208.913048E-1131.2663122
TAGCTTG450.004795782730.43600833
CAACGCA17050.030.4021845
Produced by FastQC (version 0.11.5)