FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320505
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46340.35092635014634554No Hit
CTATTCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCGA45160.3419903748944532No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36370.2754249321282388No Hit
GTATGTAGCCTGGTATCAGCAGAAACCAGGGAAAGGTCCTAAGCTCCTAA34650.2623996122695484No Hit
GGGCTATACCCAGTATGTAGCCTGGTATCAGCAGAAACCAGGGAAAGGTCCTAAGCTCCTAATCTATGGTGCATC32670.24740534871128847No Hit
CTGTTATACTTTTGACAGAAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC31270.23680334417514512No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT30600.2317295277185622No Hit
GTATAACAGTGCCCGGTGGACATTCGGACAAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT29950.2268071684696385No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29520.2235508385049659No Hit
GTAGGGGACAGCGTCACCATCACTTGCCGGGCGAGTCAGGGCTATACCCAGTATGTAGCCTGGTATCAGCAGAAA26770.20272547245182715No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26620.2015895433943832No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26530.20090798595991682No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25630.19409241161525326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25280.19144191048121742No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25130.19030598142377347No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24590.18621663681697534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24220.18341467847528029No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24220.18341467847528029No Hit
AGTCAGGGCTATACCCAGTATGTAGCCTGGTATCAGCAGAAACCAGGGAA23580.17856804783018618No Hit
CTCCTGTGCAGCCTCTGGATTCATTTTTAGTGGCAGTGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGA23500.17796221899954942No Hit
GTCTGAATAGTGTGTGGTGCCCACACGATCAGAGACTGAGACCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGAC23250.17606900390380953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22940.17372141718509207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22610.17122237325871542No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21480.16266504102597112No Hit
GTCTCTGATCGTGTGGGCACCACACACTATTCAGACTCCGTGAAGGGCCG21310.16137765476086802No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19820.1500940927902583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19800.14994263558259907No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19140.14494454772984577No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19040.14418726169154983No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG18970.14365716146474264No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18590.14077947451921802No Hit
GGACTGGAGTGGGTCTCAGTCTCTGATCGTGTGGGCACCACACACTATTC18380.13918917383879653No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18320.13873480221581894No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18170.13759887315837502No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18000.13631148689327188No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17980.1361600296856127No Hit
AATCTATGGTGCATCCACTTTACAATCAGGGGTCCCGTCTCGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA17830.13502410062816878No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17790.1347211862128504No Hit
GCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAGTGGGTCTCAGTCTCTGATCGTGTGGGCACCACA17710.1341153573822136No Hit
AAGTAATACCAGCCATTGGAAGGTCCCCCTTTCGCACAGAAATACACGGC17600.13328234274008807No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17560.1329794283247697No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTTGTCCGAATGTC17470.13229787089030334No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTTCTGTCAAAAGTATAACAGTGCC17400.13176777066349615No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17350.13138912764434818No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17230.13048038439839305No Hit
GAAATACACGGCCGTGTCCTCGTCTGTCAGACCATTCATCTGCAGATACA17230.13048038439839305No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16980.12858716930265315No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT16950.12835998349116437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16730.12669395420691326No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16590.1256337537532989No Hit
GTATTACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTGTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT16350.12381626726138864No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16150.12230169518479674No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15970.12093858031586399No Hit
GTCCTAAGCTCCTAATCTATGGTGCATCCACTTTACAATCAGGGGTCCCG15810.11972692265459048No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15810.11972692265459048No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15800.11965119405076088No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15690.11881817940863533No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT15660.11859099359714655No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGACACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGTAATACCAGC15640.11843953638948736No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15560.1178337075588506No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15420.11677350710523626No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGCGTCACCATCACTT15400.11662204989757706No Hit
GTTATACTTTTGACAGAAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC15280.11571330665162192No Hit
GCCACAGTTCGTGTGATCTCCACCTTGGTCCCTTGTCCGAATGTCCACCG15270.11563757804779233No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15000.11359290574439324No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.11336571993290445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14890.11275989110226768No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14520.10995793276057267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14350.10867054649546953No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC14270.10806471766483278No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14240.10783753185334398No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGACACGGTGACCAGGGTTCCCTGG14130.10700451721121843No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14080.10662587419207047No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGCACTGCCAC14020.1061715025690929No Hit
ATTCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCGAAG13970.10579285954994491No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATTTTTAGTGGCAGTGCC13800.10450547328484179No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTTGTCCGAATGTCCACCGGGCA13510.10230934377378352No Hit
ATCAGAGACTGAGACCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGACCC13470.10200642935846514No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13440.10177924354697636No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGAT13390.10140060052782837No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG13320.1008705003010212No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13250.10034040007421403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC900.049.97591441
TATCTCG900.049.7361136
TATATCG306.553005E-445.651225
GTGGTAT2600.042.141181
ATCTCGT1150.041.94521337
TCGTATG1100.040.8487440
TCTCCGA1350.038.04412511
GTATCAA25200.034.919071
GTAGTTT1905.275069E-1033.470170
ATGCCGT1350.033.4093244
ACGAGAC1450.033.0652922
CACGAGA1600.032.09972821
ATCTCCG1500.031.95585410
GCCTATC1400.031.91729733
CTATCTC1550.031.07446135
AGCCTAT1550.031.02723732
CTTATAC1550.030.926191
ATACGTA1450.030.6976711
GATGAAT18400.030.41413770
GTATGCC1600.030.30750342
Produced by FastQC (version 0.11.5)