FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320505
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45420.34395931859402273No Hit
CTATTCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCGA40010.3029901439222116No Hit
GGGCTATACCCAGTATGTAGCCTGGTATCAGCAGAAACCAGGGAAAGGTCCTAAGCTCCTAATCTATGGTGCATC34540.26156659762742285No Hit
GTATGTAGCCTGGTATCAGCAGAAACCAGGGAAAGGTCCTAAGCTCCTAA31830.24104414598960247No Hit
CTGTTATACTTTTGACAGAAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC31000.23475867187174607No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30990.23468294326791644No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT29150.22074888016327088No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27920.2114342618922306No Hit
GTATAACAGTGCCCGGTGGACATTCGGACAAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT27820.2106769758539347No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26450.2003021571292801No Hit
GTAGGGGACAGCGTCACCATCACTTGCCGGGCGAGTCAGGGCTATACCCAGTATGTAGCCTGGTATCAGCAGAAA25820.19553125508801555No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25430.19257783953866134No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25410.19242638233100215No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24350.18439915032506501No Hit
CTCCTGTGCAGCCTCTGGATTCATTTTTAGTGGCAGTGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGA24330.18424769311740585No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24010.1818243777948588No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22750.17228257371232977No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22640.1714495590702042No Hit
GTCTGAATAGTGTGTGGTGCCCACACGATCAGAGACTGAGACCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGAC22420.1697835297859531No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21730.16455825612171102No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21480.16266504102597112No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21420.16221066940299353No Hit
AGTCAGGGCTATACCCAGTATGTAGCCTGGTATCAGCAGAAACCAGGGAA20980.15887861083449137No Hit
GTCTCTGATCGTGTGGGCACCACACACTATTCAGACTCCGTGAAGGGCCG20740.15706112434258104No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19020.14403580448389064No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18950.14350570425708348No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18490.14002218848092207No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18140.13737168734688623No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTTGTCCGAATGTC18090.13699304432773826No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17950.1359328438741239No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17770.1345697290051912No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17670.13381244296689523No Hit
GGACTGGAGTGGGTCTCAGTCTCTGATCGTGTGGGCACCACACACTATTC17660.13373671436306564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17580.13313088553242888No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17450.13214641368264413No Hit
GAAATACACGGCCGTGTCCTCGTCTGTCAGACCATTCATCTGCAGATACA17300.1310104846252002No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17010.12881435511414194No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16880.12782988326435718No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16730.12669395420691326No Hit
AAGTAATACCAGCCATTGGAAGGTCCCCCTTTCGCACAGAAATACACGGC16530.12517938213032134No Hit
GTCCTAAGCTCCTAATCTATGGTGCATCCACTTTACAATCAGGGGTCCCG16370.12396772446904782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16230.12290752401543348No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16220.1228317954116039No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16100.12192305216564875No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG16100.12192305216564875No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16030.12139295193884159No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTTCTGTCAAAAGTATAACAGTGCC15990.1210900375235232No Hit
GCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAGTGGGTCTCAGTCTCTGATCGTGTGGGCACCACA15890.12033275148522725No Hit
AATCTATGGTGCATCCACTTTACAATCAGGGGTCCCGTCTCGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA15650.11851526499331694No Hit
GTATTACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTGTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT15630.11836380778565776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15450.11700069291672505No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGCGTCACCATCACTT15430.11684923570906584No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15410.11669777850140665No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGACACGGTGACCAGGGTTCCCTGG15310.11594049246311071No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15160.11480456340566678No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT15160.11480456340566678No Hit
GTTATACTTTTGACAGAAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC15110.11442592038651879No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15080.11419873457503No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14990.11351717714056365No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGACACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGTAATACCAGC14980.11344144853673406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14940.11313853412141567No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14760.11177541925248295No Hit
GTATAGCCCTGACTCGCCCGGCAAGTGATGGTGACGCTGTCCCCTACAGA14700.11132104762950538No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14600.11056376159120941No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14420.1092006467222767No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14340.10859481789163995No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14330.10851908928781034No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14270.10806471766483278No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14240.10783753185334398No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14010.10609577396526329No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13970.10579285954994491No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13900.10526275932313774No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTTTTCACTGTGAGGTGCAGTTGTTGGAGT13710.10382391585037543No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13710.10382391585037543No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT13700.10374818724654582No Hit
GCCACAGTTCGTGTGATCTCCACCTTGGTCCCTTGTCCGAATGTCCACCG13610.10306662981207947No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13210.10003748565889566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)