FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072844
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28990.2702163595079993No Hit
GTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAACA25440.23712673976831675No Hit
GCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTCCGCCGAACAGCCAAGCACGGAGGTCGGTGTCCCAGG23730.22118779617539922No Hit
CACCTGCACTGGGAACAGCAACAATGTTGGCCACCAAGGAGCGGCGTGGCTGCGGCTGCACCAGGGCCACCCTCC21740.202638967081887No Hit
GACTATTACTGCTCAGCCTGGGACACCGACCTCCGTGCTTGGCTGTTCGG20840.1942500494014041No Hit
GTAATAGTCAGCCTCGTCCTCAGCCTGGAGTCCACTAATGGTTAGGGAGG20310.18930990898956418No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20230.18856422741796572No Hit
ATATATTACTGTGTGAGAGGCAGTGACTTCGACTCCTGGGGACAGGGAACCCTGGTCACCGTCTCCTCAGCATCC19240.1793364179694345No Hit
GTCCCAGGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGTCCACTAATGGTTAGGGAGGCTGTGTTTC17900.16684625164515998No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17500.16311784378716757No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17050.15892338494692612No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16480.15361040374928694No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16360.1524918813918892No Hit
GCCTGGGACACCGACCTCCGTGCTTGGCTGTTCGGCGGAGGGACCAACCTGACCGTCCTGGGTCAGCCCAAGG16280.15174619982029075No Hit
CAGTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAA16170.15072088765934283No Hit
GGACTGAACAGAGAGAACTCAGCATGGCGTTTGGGCTGAACTGGCTTTTT15850.14773816137294893No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15440.14391654331850667No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14410.13431589308417627No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14300.13329058092322835No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14210.13245168915518007No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14150.1318924279764812No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13840.1290029118865371No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTTCAGCCCGGGGGGTCCCTGAGGCTCTCCTGTGCAGC13790.12853686090428804No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13750.12816402011848882No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13400.12490166324274546No Hit
ATATTACTGTGTGAGAGGCAGTGACTTCGACTCCTGGGGACAGGGAACCC13350.1244356122604964No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12920.12042757381315458No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12890.12014794322380515No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12830.11958868204510627No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTC12660.1180041087054595No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12620.11763126791966028No Hit
GCCATATATTACTGTGTGAGAGGCAGTGACTTCGACTCCTGGGGACAGGG12080.11259791731137053No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11990.11175902554332225No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11940.11129297456107319No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11930.11119976436462338No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11720.10924235023917737No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11720.10924235023917737No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11620.10831024827467926No Hit
ATATTACGGAGACTCCGTGAACGGCCGGTTCACCATATCCAGAGACAATT11470.10691209532793211No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11320.10551394238118496No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11020.10271763648769065No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10980.10234479570189142No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10830.10094664275514427No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10750.10020096118354578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.041.9707871
TCTGGAT10200.041.54922570
AATGGTA4950.037.45725670
GTATCAA21800.036.975061
ATCGTAG400.002236274835.56580457
TATCAAC25000.032.106212
ATCAACG25950.030.7997763
GTGGTAT3000.030.6097341
TCAACGC26150.030.434154
ATTAGTA450.00495696530.2318332
CAACGCA26500.030.1605345
AACGCAG27250.029.3304276
TTAACGG605.6853844E-428.51780535
TCGTATG1103.7138307E-828.1870240
TGACGGT950.00735763827.88171670
CGCAGAG28700.027.8472798
AGAGTAC28250.027.81061211
ACGCAGA29000.027.5579197
CGTAAAC500.0081318927.31815732
CAGAGTA28900.027.30152710
Produced by FastQC (version 0.11.5)