FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072844
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26270.24486318607365096No Hit
GTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAACA23200.2162476557635593No Hit
GCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTCCGCCGAACAGCCAAGCACGGAGGTCGGTGTCCCAGG22600.2106550439765707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22390.2086976298511247No Hit
GTAATAGTCAGCCTCGTCCTCAGCCTGGAGTCCACTAATGGTTAGGGAGG19530.182039513666479No Hit
GACTATTACTGCTCAGCCTGGGACACCGACCTCCGTGCTTGGCTGTTCGG19280.17970925875523375No Hit
CACCTGCACTGGGAACAGCAACAATGTTGGCCACCAAGGAGCGGCGTGGCTGCGGCTGCACCAGGGCCACCCTCC19190.17887036698718545No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19010.17719258345108888No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18330.1708542900925018No Hit
ATATATTACTGTGTGAGAGGCAGTGACTTCGACTCCTGGGGACAGGGAACCCTGGTCACCGTCTCCTCAGCATCC18230.1699221881280037No Hit
GTCCCAGGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGTCCACTAATGGTTAGGGAGGCTGTGTTTC17940.1672190924309592No Hit
GCCTGGGACACCGACCTCCGTGCTTGGCTGTTCGGCGGAGGGACCAACCTGACCGTCCTGGGTCAGCCCAAGG16950.157991282982428No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16660.15528818728538354No Hit
GGACTGAACAGAGAGAACTCAGCATGGCGTTTGGGCTGAACTGGCTTTTT16300.15193262021319034No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTTCAGCCCGGGGGGTCCCTGAGGCTCTCCTGTGCAGC16130.15034804687354358No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15140.1411202374250124No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14980.13962887428181542No Hit
CAGTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAA14720.13720540917412039No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14660.13664614799542152No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14610.13618009701317246No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14520.13534120524512416No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14040.13086711581553329No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13620.12695228756464128No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12740.118749790277058No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12560.1170720067409614No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12420.11576706399066407No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12310.11474175182971616No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12180.11353001927586863No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12160.11334359888296901No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12120.11297075809716976No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11940.11129297456107319No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11880.11073371338237432No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11870.11064050318592453No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11870.11064050318592453No Hit
ATATTACTGTGTGAGAGGCAGTGACTTCGACTCCTGGGGACAGGGAACCC11600.10812382788177965No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11580.10793740748888002No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11540.10756456670308079No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11400.10625962395278345No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTC11330.10560715257763478No Hit
ATATTACGGAGACTCCGTGAACGGCCGGTTCACCATATCCAGAGACAATT11250.1048614710060363No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11240.10476826080958648No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11110.10355652825573897No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10870.1013194835409435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.039.564641
TACGTAT700.001477100238.69685470
TCTGGAT10250.038.3193270
GTATCAA22500.038.163931
TATCAAC24750.034.2734952
GAGTTTA3950.033.510631
ATCAACG26200.032.5061873
TCAACGC26350.032.1908724
CAACGCA26650.031.9573045
GTGGTAT2900.031.5995461
AACGCAG27200.031.0645126
CGGGTAT553.5719652E-430.87348617
AGTTTAT4350.030.4206832
AATGGTA4900.030.40466970
TTAGCGT450.00501441530.1606773
AGAGTAC28050.030.15408511
CGCAGAG28300.029.996548
ACGCAGA28450.029.7093337
CAGAGTA28500.029.55877910
ACGGGTA1152.0099833E-929.53943316
Produced by FastQC (version 0.11.5)