FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078383
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65400.606463566283964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56430.5232834716422644No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTA53970.5004715393325007No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42200.3913266436878178No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37840.35089573926888684No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37550.348206527736435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36170.3354095900992505No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35200.32641464118035984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35040.32493093826590363No Hit
GTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCGCAGCCTCCACCC33770.31315404638240774No Hit
GCTACACACTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGA33180.3076828918853506No Hit
CGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCCAG32160.29822428580569244No Hit
CTCGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCC31980.2965551200269292No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30750.2851491538720473No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC30470.28255267377174903No Hit
GAACATTAGCAGGGTGGAGGCTGCGGATGTTGGGGTTTATTACTGCATGCAAGCTACACACTGGCCTCCGGGGTA29820.27652513068177076No Hit
GTCTGGGACCCCAGAGTCCCGCTGAGAAAGCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA29610.27457777060654703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29510.27365045628501194No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28990.26882842181302935No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27500.2550114384221561No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA26870.24916935819648492No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26360.24444005515665582No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25740.23869070636313816No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25710.2384125120666776No Hit
GATTTCACACTGAACATTAGCAGGGTGGAGGCTGCGGATGTTGGGGTTTATTACTGCATGCAAGCTACACACTGG24620.2283047859619449No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTTCTCAGCGGGAC24230.22468826010795792No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23960.22218451143981313No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23960.22218451143981313No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23950.22209178000765964No Hit
GTATACGAGGCTTTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA23840.22107173425397098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23700.21977349420382186No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23280.21587877405337433No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22780.21124220244569877No Hit
GTAATAAACCCCAACATCCGCAGCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG22430.2079966023203259No Hit
GCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGT22230.20614197367725567No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTATA21930.20336003071265035No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC21790.20206179066250118No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCCCGGAGG21580.20011443058727743No Hit
GCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGT19690.1825881899102638No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19670.18240272704595678No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19550.18128994986011462No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19490.18073356126719356No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19180.17785888687043472No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18720.1735932409913732No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.17331504669491266No Hit
GGCCAAAAGTGTACCCCGGAGGCCAGTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCGCAGCCTCCACC18640.1728513895341451No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGA17800.16506194923325015No Hit
GTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTT17800.16506194923325015No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17790.16496921780109663No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA17430.16163088624357025No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC17150.1590344061432719No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17060.15819982325389031No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA17000.15764343466096925No Hit
GTGTAGCTTGCATGCAGTAATAAACCCCAACATCCGCAGCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGC16830.15606700031435955No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16820.15597426888220603No Hit
CTGTATACGAGGCTTTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCC16800.15578880601789902No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGGGGTACACTTTTGGCC16790.1556960745857455No Hit
GTTTATTACTGCATGCAAGCTACACACTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16510.1530995944854472No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTC16450.15254320589252612No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15800.14651566280254788No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGATGTTTCCATCACTGTAT15800.14651566280254788No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15690.14549561704885927No Hit
CCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCC15650.14512469132024522No Hit
GGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGG15570.14438283986301712No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15530.1440119141344031No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15530.1440119141344031No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15460.1433627941093285No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACT15430.14308459981286797No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15220.14113723973764422No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14840.1376134453158108No Hit
AGCTACACACTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG14700.13631520526566163No Hit
CAGTAATAAACCCCAACATCCGCAGCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG14560.13501696521551249No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14560.13501696521551249No Hit
GCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCC14500.13446057662259142No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14310.1326986794116747No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAACATTAGCAGGGTGGAGGCT14020.13000946787922288No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13960.1294530792863018No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13810.12806210780399913No Hit
GGGTGGAGGCTGCGGATGTTGGGGTTTATTACTGCATGCAAGCTACACAC13700.1270420620503105No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13630.12639294202523593No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13270.12305461046770952No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13230.12268368473909548No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13140.12184910184971388No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13070.1211999818246393No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12990.12045813036741121No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12990.12045813036741121No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12790.11860350172434098No Hit
GTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCAT12760.11832530742788044No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12500.11591429019188915No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12470.11563609589542861No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAA12390.11489424443820054No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12290.11396693011666542No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12120.11239049577005573No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12050.11174137574498115No Hit
CCCTTAGACTCTCCTGTGCAGCCTCCGGATTCGCCTTCAATGACGCCTGG12000.11127771858421359No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11950.11081406142344602No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11830.1097012842376039No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11700.10849577561960824No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11600.10756846129807314No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11490.1065484155443845No Hit
ACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAACATTAGCAGGGTGGAGG11390.10562110122284939No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11330.10506471262992834No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11270.10450832403700726No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11180.10367374114762565No Hit
GTATACAGTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCCAGG11110.10302462112255109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG200.007732558551.105733
CGACTCA200.007732558551.105736
TACGTAT400.00965044948.3163170
GTGGTAT1900.046.622771
GGTATCA6750.041.3893281
GTATCAA17650.039.7649841
CTACGCT702.401066E-839.01730728
ACTACGC702.4108886E-838.99918727
TGGTATC2350.037.6950042
GTAAGAT400.002440733834.93723349
AATGGTA4800.033.55299470
TATCAAC21050.033.3421362
TGTTAGA13500.032.9266770
TTGCCGA450.004093430531.43801756
TTTGCCG450.004132154431.377655
ATCAACG22600.031.2061523
TCAACGC22750.031.0003974
CAACGCA23200.030.3990965
ACGGGGC1351.0913936E-1130.28768516
AACGCAG23650.029.964746
Produced by FastQC (version 0.11.5)