FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078383
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64540.598488663118762No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44380.41154209589728324No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTA43130.3999506668780944No Hit
GTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCGCAGCCTCCACCC41540.38520636916568607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41230.3823316947689272No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41040.38056979755801046No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38910.36081800250931256No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32150.29813155437353894No Hit
CTCGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCC31890.2957205371375476No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31600.29303132560509576No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28850.2675301817628802No Hit
GCTACACACTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGA28700.2661392102805775No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28140.26094625007998085No Hit
GTCTGGGACCCCAGAGTCCCGCTGAGAAAGCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA28050.26011166719059925No Hit
GAACATTAGCAGGGTGGAGGCTGCGGATGTTGGGGTTTATTACTGCATGCAAGCTACACACTGGCCTCCGGGGTA27810.257886112818915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27730.2571442613616869No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27220.25241495832185784No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27030.2506530611109411No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26430.24508917518173043No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG26360.24444005515665582No Hit
CGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCCAG25360.2351669119413047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24950.23136492322301078No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24910.23099399749439672No Hit
GTAATAAACCCCAACATCCGCAGCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG24870.23062307176578267No Hit
GCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGT24310.22543011156518603No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA24180.22422460294719038No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24130.2237609457864228No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA23980.22236997430412014No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23550.21838252272151915No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23380.21680608837490947No Hit
GATTTCACACTGAACATTAGCAGGGTGGAGGCTGCGGATGTTGGGGTTTATTACTGCATGCAAGCTACACACTGG23070.2139314139781506No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22430.2079966023203259No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTATA22280.20660563083802322No Hit
GTATACGAGGCTTTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA22060.20456553933064597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21810.2022472535268082No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21740.20159813350173358No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21740.20159813350173358No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21640.20067081918019852No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTTCTCAGCGGGAC21540.1997435048586634No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21490.1992798476978958No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC20190.18722476151793935No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCCCGGAGG19780.18342277279964542No Hit
GGCCAAAAGTGTACCCCGGAGGCCAGTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCGCAGCCTCCACC19590.18166087558872868No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19510.1809190241315006No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA18540.17192407521261No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18020.1671020407406274No Hit
GGGTGGAGGCTGCGGATGTTGGGGTTTATTACTGCATGCAAGCTACACAC17690.16404190347956155No Hit
GTGTAGCTTGCATGCAGTAATAAACCCCAACATCCGCAGCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGC17510.16237273770079833No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17270.16014718332911404No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17050.1581070918217368No Hit
GCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGT16890.15662338890728061No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16890.15662338890728061No Hit
GTTTATTACTGCATGCAAGCTACACACTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16720.15504695456067094No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16150.1497612629279208No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16120.14948306863146024No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGGGGTACACTTTTGGCC16090.1492048743349997No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16080.14911214290284622No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15870.14716478282762246No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15820.14670112566685492No Hit
GGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGG15760.14614473707393383No Hit
GCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCC15660.14521742275239874No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC15660.14521742275239874No Hit
CTGTATACGAGGCTTTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCC15660.14521742275239874No Hit
GTGATGGAAACATCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTT15220.14113723973764422No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGA15190.14085904544118372No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15060.13965353682318804No Hit
CAGTAATAAACCCCAACATCCGCAGCCTCCACCCTGCTAATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG14980.13891168536595996No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTC14960.13872622250165292No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14830.1375207138836573No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14380.1333477994367493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14330.13288414227598172No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14160.13130770792937205No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14050.1302876621756834No Hit
AGCTACACACTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG14020.13000946787922288No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGATGTTTCCATCACTGTAT13970.1295458107184553No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13760.12759845064323158No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13550.12565109056800783No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACT13480.12500197054293324No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13420.12444558195001218No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13410.12435285051785869No Hit
CCAGTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCC13330.1236109990606306No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13230.12268368473909548No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13150.1219418332818674No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12640.11721253024203832No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAACATTAGCAGGGTGGAGGCT12600.11684160451342428No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12500.11591429019188915No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12220.11331781009159084No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAA12090.11211230147359519No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11850.10988674710191093No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11840.10979401566975741No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11770.10914489564468283No Hit
GTATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCAT11750.1089594327803758No Hit
GTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTT11660.10812484989099419No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11560.10719753556945909No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11470.10636295268007748No Hit
CCCTTAGACTCTCCTGTGCAGCCTCCGGATTCGCCTTCAATGACGCCTGG11270.10450832403700726No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11250.10432286117270023No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11240.10423012974054673No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11090.10283915825824406No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11060.10256096396178353No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10940.10144818677594138No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10870.10079906675086679No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10820.10033540959009926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAA252.6792282E-454.72432331
CGATAGC200.007512971851.47947335
GATCTAG200.007809400650.978051
GTGGTAT2400.048.1459351
TGGTATC2550.045.3033142
GGTATCA7400.041.7928161
GTATCAA19000.037.026161
GTAATGA501.4684517E-435.9196362
AATGGTA4500.034.99161570
ATCGCGC500.00255817634.60328369
CCGAAAC450.002611940534.45723368
GACAGGT1201.8189894E-1233.9853671
CCTATAC701.0969306E-633.9774863
ACGGGAC1850.033.0591773
GAGTTTA2500.032.6259541
TATCAAC21950.031.8877562
ATTCACT1307.2759576E-1231.3638322
ATCAACG22600.031.1209743
TCAACGC22950.030.4983124
GGGTTAC450.00497515230.2092151
Produced by FastQC (version 0.11.5)