FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478256
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55930.37835124633351735No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48890.3307275600437272No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46150.31219220486843957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39280.2657185223668972No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36550.24725081447327119No Hit
GTAATACACGCCCGTGTCTGCGGCGGTCAGAGAGTTCAAAGTCAGGGAGA35900.242853741165265No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35820.24231256291197192No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT34750.23507430377417712No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33790.2285801647346603No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33340.22553603705988676No Hit
GATCTATGGTGCATCCAGTTTGCTAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGTTCTGGGACAGATTTCA33030.22343897132837612No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31950.21613306490891968No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30880.20889480577112488No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30360.20537714712471994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30190.20422714333647218No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28740.19441828749553527No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28570.1932682837072875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26880.1818358931064714No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26460.1789947072766828No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26380.17845352902338973No Hit
GGGTACCAGAGCCCTGTTGACAATAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT24490.16566819278934095No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24300.1643828944377699No Hit
CTGGTACCCTCCGGACCTTTGGCCAGGGGACCAAGGTGGAGATTAAACGA23720.16045935210139517No Hit
CTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA23540.15924170103148574No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23410.1583622863698845No Hit
CAGTAATACACGCCCGTGTCTGCGGCGGTCAGAGAGTTCAAAGTCAGGGAGAACTGGTTTTTGGACCTGTCCA23390.15822699180656125No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC23320.1577534608349298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23300.15761816627160655No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23190.15687404617332856No Hit
GTCTCCATCCTCCCTGTCCGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTGA22660.15328874024526198No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22250.150515201697135No Hit
CCTTTGGCCAGGGGACCAAGGTGGAGATTAAACGAACTGTGGCTGCACCA22170.14997402344384192No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22020.14895931421891742No Hit
GTACCAGAGCCCTGTTGACAATAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT21300.1440887099392798No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21050.14239752789773896No Hit
GGCCAAAGGTCCGGAGGGTACCAGAGCCCTGTTGACAATAATAAGTCGCA20730.14023281488456668No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCCCCTGGCCAAAGGTC20520.13881222196967238No Hit
GAGTATTAGTGACTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATC20310.13739162905477806No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20060.13570044701323722No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGTTCTGGGACAGATTTCACT19830.13414455953501964No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTATTATTGTCAACAGGGCTCTGGTACC19780.13380632312671148No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19640.1328592611834486No Hit
GTTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGAT19410.13130337370523104No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19320.13069454817027634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCC19310.1306269008886147No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19010.12859748243876568No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18880.12771806777716446No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18740.12677100583390158No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18490.12507982379236074No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18490.12507982379236074No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17940.12135922330097086No Hit
GTCTCCCACAGATGCGGACAGGGAGGATGGAGACTGGGTCATCTGGATGT17780.12027686679438472No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTGACTTTTTAAATTGGTATCAGCAGAAA17520.11851803747118227No Hit
GAGTGAAATCTGTCCCAGAACCACTGCCACTGAACCTTGATGGGACTCCA17460.11811215378121245No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17180.11621802989468671No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17120.1158121462047169No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16890.11425625872649933No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16860.11405331688151443No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16720.11310625493825155No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16540.11188860386834217No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16470.11141507289671071No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16430.11114448377006418No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16420.11107683648840255No Hit
GTGTATTACTGTGCGACCTCGATTAATTACAATTTTTGGCGTTATAACGAACAGAGGGCATACTACTTTGACTA16400.11094154192507928No Hit
CTCCGGACCTTTGGCCAGGGGACCAAGGTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16210.10965624357350826No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16130.10911506532021518No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16120.10904741803855354No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16100.10891212347523026No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15700.10620623220876492No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15680.10607093764544166No Hit
GTCCCAGAACCACTGCCACTGAACCTTGATGGGACTCCACTTAGCAAACT15670.10600329036378002No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15610.1055974066738102No Hit
GTATTACTGTGCGACCTCGATTAATTACAATTTTTGGCGTTATAACGAAC15580.10539446482882532No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15560.10525917026550206No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15370.10397387191393101No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15350.10383857735060775No Hit
GGACTCCACTTAGCAAACTGGATGCACCATAGATCAGGAGCTTAGGGGCT15350.10383857735060775No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA15260.10322975181565304No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15140.10241798443571344No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15140.10241798443571344No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTCACTA15090.10207974802740527No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15070.10194445346408199No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGAACCACTGCCACTGAACCT14930.10099739152081913No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14920.10092974423915749No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14880.10065915511251096No Hit
GCTCTGGTACCCTCCGGACCTTTGGCCAGGGGACCAAGGTGGAGATTAAA14810.10018562414087953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)