FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478256
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49710.3362746371399812No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46050.3115157320518232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43270.29270978774988904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43100.2915597839616413No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36370.24603316340336182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35410.23953902436384494No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35180.2379831368856274No Hit
GTAATACACGCCCGTGTCTGCGGCGGTCAGAGAGTTCAAAGTCAGGGAGA32670.22100366918855735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31930.21599777034559645No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT31620.21390070461408578No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31160.21078892965765064No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30630.20720362372958406No Hit
GATCTATGGTGCATCCAGTTTGCTAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGTTCTGGGACAGATTTCA30420.20578303081468974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29160.19725947332532387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28150.19042709787749887No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27820.188194737582665No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27470.1858270827245078No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27160.18373001699299715No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26730.1808211838815469No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26350.1782505871784048No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24800.16776525852085158No Hit
GGGTACCAGAGCCCTGTTGACAATAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT24520.16587113463432585No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23970.16215053414293598No Hit
CTGGTACCCTCCGGACCTTTGGCCAGGGGACCAAGGTGGAGATTAAACGA23880.1615417086079813No Hit
GTCTCCATCCTCCCTGTCCGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTGA23730.16052699938305678No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23190.15687404617332856No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23180.15680639889166695No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC23150.15660345704668202No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTGACTTTTTAAATTGGTATCAGCAGAAA23120.15640051520169712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22270.15065049626045826No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21530.14564459741749738No Hit
CAGTAATACACGCCCGTGTCTGCGGCGGTCAGAGAGTTCAAAGTCAGGGAGAACTGGTTTTTGGACCTGTCCA21300.1440887099392798No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGTTCTGGGACAGATTTCACT21130.14293870615103205No Hit
CCTTTGGCCAGGGGACCAAGGTGGAGATTAAACGAACTGTGGCTGCACCA20910.14145046595447608No Hit
CTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA20900.14138281867281444No Hit
GGCCAAAGGTCCGGAGGGTACCAGAGCCCTGTTGACAATAATAAGTCGCA20870.14117987682782954No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCCCCTGGCCAAAGGTC20800.14070634585619812No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20710.14009752032124342No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20700.14002987303958178No Hit
GAGTATTAGTGACTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATC20190.13657986167483846No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20140.1362416252665303No Hit
GTACCAGAGCCCTGTTGACAATAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT19640.1328592611834486No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19600.13258867205680208No Hit
GTTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGAT19420.13137102098689266No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19320.13069454817027634No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18630.1260268857356236No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18500.12514747107402235No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18500.12514747107402235No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTATTATTGTCAACAGGGCTCTGGTACC18420.1246062928207293No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18090.12237393252589539No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17890.12102098689266272No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCC17670.11953274669610676No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17640.11932980485112187No Hit
GTCTCCCACAGATGCGGACAGGGAGGATGGAGACTGGGTCATCTGGATGT17280.11689450271130306No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17260.11675920814797978No Hit
GAGTGAAATCTGTCCCAGAACCACTGCCACTGAACCTTGATGGGACTCCA17210.11642097173967161No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17120.1158121462047169No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17090.115609204359732No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17050.11533861523308547No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16890.11425625872649933No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16590.11222684027665031No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16370.11073860008009438No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15930.10776211968698252No Hit
GTCCGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15890.10749153056033595No Hit
GTCCCAGAACCACTGCCACTGAACCTTGATGGGACTCCACTTAGCAAACT15880.10742388327867433No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGAACCACTGCCACTGAACCT15800.10688270502538126No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15720.1063415267720882No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15690.10613858492710328No Hit
GTGTATTACTGTGCGACCTCGATTAATTACAATTTTTGGCGTTATAACGAACAGAGGGCATACTACTTTGACTA15640.10580034851879513No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15610.1055974066738102No Hit
CTCCGGACCTTTGGCCAGGGGACCAAGGTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15530.10505622842051715No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15520.10498858113885552No Hit
GGACTCCACTTAGCAAACTGGATGCACCATAGATCAGGAGCTTAGGGGCT15260.10322975181565304No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14970.10126798064746566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG300.002131682870.7566270
TACGTAT502.2667233E-456.60529770
CGATTGC304.2341516E-449.8796863
GTGGTAT3000.036.7843441
AATGGTA5500.036.02155370
TATAAGA400.003030670633.4290052
GTATCAA18300.031.4302431
GTACGTA600.004754105630.4912669
TCTAAAG450.00539393429.7146682
GAGTAGG704.7650086E-528.6631261
TATCAAC21300.026.8373682
GTACCTA500.00900705726.7522541
TCAACGC21950.026.1949374
CAACGCA21850.026.1644845
ATCAACG22100.025.865883
TGGTACG750.001006824425.86096667
AACGCAG22000.025.8419916
ACCTAAC650.001040798425.7146193
GAGTTTA3900.024.8658771
ACGCAGA23150.024.7069057
Produced by FastQC (version 0.11.5)