FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950081
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44180.46501298310354594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34150.35944303696211166No Hit
CTATTACTGTCAGCAGTATTCTATCTCACCTCCGACGTTCGGCCAAGGGA32750.34470745125941893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32270.3396552504470672No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA32180.3387079627947512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29900.3147100089360802No Hit
GTCTATTACTGTCAGCAGTATTCTATCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA27650.2910278176281812No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27040.28460731242915077No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26250.27629223192548846No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT25850.27208206458186196No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25110.26429325499615297No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24130.2539783450042681No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23530.24766309398882833No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23200.24418970593033645No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22860.24061106368825397No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22520.23703242144617145No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC22490.23671665889539945No Hit
GTATTCTATCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT22050.23208547481741032No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG21040.22145480227475342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21010.2211390397239814No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC20290.21356073850545376No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20280.21345548432186306No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20120.2117714173844125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19740.20777175840796733No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19610.2064034540212887No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA19510.20535091218538207No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19410.20429837034947546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19040.200403965556621No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18740.19724634004890113No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18440.19408871454118123No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18110.19061532648268936No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17990.1893522762796014No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17520.18440532965084028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17000.17893211210412585No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATTCTATCTCA16640.175142961494862No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG16130.16977499813173824No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA15960.167985677010697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15290.1609336467101226No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14710.1548289040618642No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14650.15419737896032024No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14510.15272382039005095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13990.14725060284333652No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT13860.1458822984566579No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13640.14356670641766334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13540.14251416458175672No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGC13480.14188263948021274No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGACTGTGCTCGTGGACGTGTCCCTGGT13470.1417773852966221No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGAAGAAATTGTGTTGACGCAGTC13380.1408300976443061No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACCGAAGAAATTGTGTTGACGCAGTCTC13330.1403038267263528No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13290.13988280999199015No Hit
ATTCTATCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAA13170.13861975978890223No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12920.13598840519913566No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12900.13577789683195432No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12840.13514637173041036No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12780.13451484662886637No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12170.12809434142983597No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12000.12630502030879473No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11970.12598925775802275No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11710.12325264898466552No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11490.12093705694567095No Hit
GCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGG11460.12062129439489896No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11320.11914773582462967No Hit
AGATAGCACCGAAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCT11190.11777943143795107No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT10950.11525333103177518No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10920.1149375684810032No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10850.11420078919586857No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10720.11283248480918995No Hit
ATAGAATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC10690.11251672225841797No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCC10670.11230621389123664No Hit
GTGTATTACTGTGCGAGAGATGGGATCGTTAGAGATGGCTACAATTACGG10610.11167468878969267No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG10530.11083265532096738No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10520.11072740113737671No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10480.11030638440301405No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10400.10946435093428877No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10380.10925384256710745No Hit
GATAGAATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT10320.10862231746556347No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10140.10672774216093153No Hit
GAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG9560.10062299951267312No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACCGAAGAAATTGTGTTGACGCAGTCTCCAGGCACC9510.10009672859471981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG150.002575100667.465526
GGTATCA7950.044.979381
GTGGTAT1550.041.3520131
TGGTATC1700.037.6953742
ATAATAC451.1442686E-437.4749263
AATGGTA5350.037.24049870
GTATCAA18100.035.2255671
TACTGGG5900.034.9333770
TATCAAC20200.030.8889872
ATCAACG20250.030.8127173
TCAACGC20550.030.0346494
CAACGCA20750.029.7467235
AACGCAG21450.028.9362626
CGCAGAG22500.027.4533218
ACGCAGA22500.027.4460937
AGAGTAC23050.026.80813411
GATTCAC1655.456968E-1226.5787261
GTCGTCA4750.026.3065116
GCACGGA550.00992216426.22531759
TGTCGTC4900.025.4985215
Produced by FastQC (version 0.11.5)