FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916734
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCGCACCTCGGACGTTCGGCCAGGGGA45470.4959999301869463No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39910.43534983975722513No Hit
GTATGGTAGCGCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT32890.3587736464448793No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29390.32059463268516275No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA27810.30335953504506213No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA27510.30008704815137216No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGACGTCAGGGTCAATGAGA26260.2864516860943305No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC24050.26234436597748095No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23170.25274507108932365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22420.24456385385509863No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21820.23801888006771865No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21700.23670988531024265No Hit
GTGTATTACTGTCAGCAGTATGGTAGCGCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA21190.2311466575909697No Hit
ATATATTACTGTGCGGCGGACTCCGGCGACTATTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA19790.21587505208708305No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19740.21532963760480137No Hit
CATCTATGGTGCATCCAGTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA19480.21249348229693674No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19280.2103118243678101No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18590.2027851045123231No Hit
TCCCAGGGAAGGGACTGGAGTGGATTGGCTTCACCTCTTACATTGGGAAG18390.20060344658319645No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18200.19853087155052612No Hit
GTCTTTATATCCGGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGA17180.18740441611198014No Hit
GTCATCAGCAGGTACTTAGCCTGGTACAATCAGAAACCTGGCCAGGCTCC17050.18598633845804782No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16810.1833683489430958No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16200.1767142922592595No Hit
ATCCAGTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCACTCTCACCATCAG15800.1723509764010062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15710.1713692303328992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15180.16558783682071354No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15160.16536967102780087No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14300.15598854193255624No Hit
GTAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA13970.15238880634949725No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13960.1522797234530409No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC13760.15009806552391425No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCGCA13730.14977081683454524No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC13520.14748007600896226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13430.14649832994085527No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13320.1452984180798356No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13210.14409850621881592No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13180.14377125752944692No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13150.14344400884007794No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12970.14148051670386394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12910.14082601932512592No Hit
GTACTTAGCCTGGTACAATCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGTAGGGCCC12800.13962610746410625No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12550.13689903505269796No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12190.13297205078026997No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGATTGTACCAGGCTAAGTACCTGCTGATGACAGTCTGA12120.13220847050507564No Hit
CTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATT11990.1307903928511433No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11940.13024497836886165No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11940.13024497836886165No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11930.13013589547240528No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11660.1271906572680843No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCCTCCTTCTCCTGCTGGCAGCTCCCAGATGGGTC11580.12631799409643366No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11430.12468175064958865No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC11400.12435450196021966No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11380.12413633616730699No Hit
CAGTAATATATGGCCGTGTCCGCAGCGGTCACAGACGTCAGGGTCAATGA11130.12140926375589867No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11060.12064568348070433No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11030.12031843479133532No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11030.12031843479133532No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10970.11966393741259733No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10970.11966393741259733No Hit
ATCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGT10920.11911852293031568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10760.11737319658701434No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10700.11671869920827634No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10600.11562787024371302No Hit
TCTATGGTGCATCCAGTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCACT10570.11530062155434401No Hit
GGGCCAGTCAGACTGTCATCAGCAGGTACTTAGCCTGGTACAATCAGAAA10540.11497337286497501No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10520.11475520707206234No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTGC10490.11442795838269335No Hit
CTGCTACTCTGGCTCCCAGATATTACCGGAGAAGTTGTGTTGACGCAGTCTCCAGGCACC10220.11148272017837235No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA10160.11082822279963435No Hit
GTGCAGGGCCAGTCAGACTGTCATCAGCAGGTACTTAGCCTGGTACAATCAGAAACCTGGCCAGGCTCCCAGGCT9960.10864656487050768No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCACTCTCAC9940.10842839907759504No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9780.10668307273429369No Hit
ATATTACTGTGCGGCGGACTCCGGCGACTATTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTC9770.10657398983783736No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9610.10482866349453604No Hit
CTACTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTG9430.10286517135832204No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9390.1024288397724967No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTACCGGAGAAGTTGTGTTGACGCAGTCTC9290.10133801080793338No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA9230.10068351342919539No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9190.10024718184337006No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9170.10002901605045739No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9170.10002901605045739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)