FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916734
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCGCACCTCGGACGTTCGGCCAGGGGA42120.45945715987407465No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40910.44625812940285836No Hit
GTATGGTAGCGCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT30410.3317210881237087No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA26210.2859062716120489No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25030.27303448983020157No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA24780.27030741741879327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24500.2672530963180159No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGACGTCAGGGTCAATGAGA24270.26474418969952024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23300.25416314874325596No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC22020.24020053799684535No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21900.23889154323936934No Hit
GTGTATTACTGTCAGCAGTATGGTAGCGCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA20530.22394718642485173No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19070.20802108354222706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.20387593347688643No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18690.20387593347688643No Hit
ATATATTACTGTGCGGCGGACTCCGGCGACTATTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA18450.20125794396193444No Hit
GTCTTTATATCCGGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGA17610.19209498065960245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17300.18871341086945614No Hit
TCCCAGGGAAGGGACTGGAGTGGATTGGCTTCACCTCTTACATTGGGAAG17240.18805891349071813No Hit
CATCTATGGTGCATCCAGTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA17010.18555000687222248No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16340.17824145280964818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16260.1773687896379975No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15310.16700591447464586No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15230.1661332513029952No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14800.16144268675537288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14650.15980644330852786No Hit
GTCATCAGCAGGTACTTAGCCTGGTACAATCAGAAACCTGGCCAGGCTCC14640.15969736041207155No Hit
ATCCAGTAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCACTCTCACCATCAG14380.1568612051042069No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC14100.15380688400342957No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13700.14944356814517626No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13690.1493344852487199No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCGCA13680.1492254023522636No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13660.14900723655935091No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13530.1475891589054186No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13450.1467164957337679No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13430.14649832994085527No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13330.14540750097629193No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCCTCCTTCTCCTGCTGGCAGCTCCCAGATGGGTC12930.1410441851180386No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12660.1380989469137176No Hit
GTAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA12570.13711720084561063No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12540.13678995215624162No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12380.13504462581294027No Hit
GTACTTAGCCTGGTACAATCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGTAGGGCCC12210.13319021657318264No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12120.13220847050507564No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12110.13209938760861928No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC11950.13035406126531796No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11950.13035406126531796No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11800.12871781781847297No Hit
CTGCTACTCTGGCTCCCAGATATTACCGGAGAAGTTGTGTTGACGCAGTCTCCAGGCACC11710.12773607175036597No Hit
CTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATT11500.125445330924783No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11400.12435450196021966No Hit
GGGCCAGTCAGACTGTCATCAGCAGGTACTTAGCCTGGTACAATCAGAAA11170.121845595341724No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11110.121191097962986No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11010.12010026899842267No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10960.119554854516141No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10870.11857310844803401No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGTCTGC10800.11780952817283968No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10780.11759136237992701No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGATTGTACCAGGCTAAGTACCTGCTGATGACAGTCTGA10770.11748227948347068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC10670.11639145051890734No Hit
ATCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGT10160.11082822279963435No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9870.10766481880240068No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9760.10646490694138104No Hit
GTGCAGGGCCAGTCAGACTGTCATCAGCAGGTACTTAGCCTGGTACAATCAGAAACCTGGCCAGGCTCCCAGGCT9730.10613765825201205No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9690.1057013266661867No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9560.10428324901225437No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9480.1034105858406037No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9420.10275608846186571No Hit
CAGTAATATATGGCCGTGTCCGCAGCGGTCACAGACGTCAGGGTCAATGA9320.10166525949730237No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTACCGGAGAAGTTGTGTTGACGCAGTCTC9310.10155617660084605No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9290.10133801080793338No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG9280.10122892791147706No Hit
GTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTACT9280.10122892791147706No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCACTCTCAC9190.10024718184337006No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCA9170.10002901605045739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)