FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549052
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64640.4172874764694794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64030.41334958413274697No Hit
CTACAAACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG55930.3610595383499069No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53740.3469218593049168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49090.3169034996888419No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46360.2992798175916625No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45200.2917913665906632No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40860.2637742309489933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39330.25389722230112355No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38250.24692521619674485No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37020.23898487591120246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34810.22471808564205722No Hit
GGGTTTGTAGAGCTTGCATACAATAATAAACCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT33260.21471196577003224No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32510.2098702948642137No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31220.20154262090620587No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30730.19837939591440443No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30390.1961845051037667No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29740.19198839031872397No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29570.1908909449134051No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29250.18882516532692253No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG28260.18243415973124208No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG27680.17868993423074242No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26000.16784459140170893No Hit
GTAATGGATACAAGTATTTGGATTGGTATCTACAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTT25260.16306747610796796No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24790.16003336234032167No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAAGTATTTGGATTGGTATCTACAGAAGCCAGGGCAGTCTC24070.1553853582707359No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23690.15293224501178787No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23270.15022090930452947No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGAAACA23080.14899435267505545No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23020.14860701900258996No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22960.14821968533012447No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22570.14570201645909886No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA22350.14428179299339208No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAACCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGTAGATA22260.14370079248469386No Hit
CAATAATAAACCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA22170.14311979197599564No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC21870.14118312361366825No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21340.13776167617355647No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21190.13679334199239276No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21090.13614778587161697No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20860.1346630067938326No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20850.13459845118175504No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20720.13375922822474648No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20660.133371894552281No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20600.13298456087981553No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20270.13085422568125538No Hit
GGTATCTACAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTT20000.12911122415516071No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATATTGGGGTTTATTATTGTATGCAAGCTCTACAAACC19970.12891755731892798No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTAATCGGGCC19870.12827200119815216No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT19710.12723911140491087No Hit
TTATTATTGTATGCAAGCTCTACAAACCCCGATCACCTTCGGCCAAGGGA19450.12556066549089379No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG19380.12510877620635072No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19240.1242049976372646No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18830.1215582175420838No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18810.12142910631792864No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18730.120912661421308No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18730.120912661421308No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA18430.11897599305898059No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18430.11897599305898059No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18010.11626465735172223No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17870.1153608787826361No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC17820.1150381007222482No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17800.11490898949809303No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC17480.11284320991161045No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17430.11252043185122255No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17430.11252043185122255No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG17350.11200398695460191No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17220.11116476399759337No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17100.1103900966526624No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16560.10690409360047307No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16540.1067749823763179No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16530.10671042676424032No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16510.10658131554008515No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16480.10638764870385242No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15980.10315986809997339No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15930.10283709003958551No Hit
GCATAGTAATGGATACAAGTATTTGGATTGGTATCTACAGAAGCCAGGGC15740.10161053341011149No Hit
GTAGATACCAATCCAAATACTTGTATCCATTACTATGCAGGAGGCTCTGA15590.10064219922894778No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15570.10051308800479261No Hit
GTCTATTACTGTGCGAGAGATCTGGGTCTGGAAATCATGTATGCTTTTGA15520.10019030994440471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)