FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379574
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51810.37555071348111807No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG47570.34481658830914474No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40240.291684244556653No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38430.2785642524431455No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAAAGACATCACCATAGTAGAGCAGGCAGTAATAGT35210.2552237139870714No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAAAGACATCACCATAGTAGAGCAGG34030.24667034896279577No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33320.2415238327193757No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC30560.2215176568998836No Hit
GACTATTACTGCCTGCTCTACTATGGTGATGTCTTTTGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT30050.21782086354193397No Hit
CTACTATGGTGATGTCTTTTGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC29060.21064473525885524No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28730.20825269249782905No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28720.2081802063535555No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27320.19803214615526243No Hit
CCATAGTAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCG26460.19179833774773952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26250.19027612871799557No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26140.1894787811309868No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26050.1888264058325251No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26020.18860894739970455No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA25870.18752165523560171No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25800.18701425222568704No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25100.1819402221265405No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT23370.1694001191672212No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22980.16657315954055382No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22710.16461603364516872No Hit
GTTCTCAGGCTCCCTCCTTGGCGGCAAAGCTGCCCTGACACTGTCAGGTG22410.16244144931696305No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22340.1619340463070484No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22130.16041183727730446No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21740.15758487765063708No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21250.1540330565812345No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG20970.15200344454157588No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20720.15019129093473782No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20680.14990134635764374No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20420.14801670660653216No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20010.14504477469131774No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19860.14395748252721494No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTACTATGGTGATGTCT19660.14250775964174447No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC19620.1422178150646504No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19350.1402606891692653No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG17850.129387767528237No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17670.12808301693131358No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17550.1272131832000313No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17390.12605340489165495No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17100.12395130670772281No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16660.12076191635968785No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAAAGACATCAC16650.12068943021541433No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16560.12003705491695263No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16510.11967462419558501No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16330.11836987359866162No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTG16080.11655771999182357No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT16040.11626777541472948No Hit
CTATTACTGCCTGCTCTACTATGGTGATGTCTTTTGGGTTTTCGGCGGAG15930.11547042782772074No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15920.11539794168344722No Hit
ACTATGGTGATGTCTTTTGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT15910.11532545553917367No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGCTTA15820.11467308024071197No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15740.1140931910865238No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCGCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC15180.11003396700720657No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14880.10785938267900091No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA14490.10503242305233355No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13950.10111817126156335No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA13870.10053828210737517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG305.2238296E-447.7998860
GGTATCA13450.047.750721
GACCGAT1950.041.86414726
CTTATAC2200.041.7043651
TCGTATG1350.040.877240
CGTCTAA350.001240237240.1018550
GTGGTAT3150.038.835811
CGTATGC1700.038.63450641
ACGAGAC2250.037.76524722
GTATCAA34600.037.320541
TATACAC2650.037.187123
ACCGATC2150.036.40226427
AGGGGGG3100.035.80714470
TCTCGTA1550.035.5060738
TGGTATC3550.035.4171642
TATCTCG1650.035.34415436
TGTGTCG23000.034.35104870
TATCAAC39150.032.896362
TCAGTAT2500.031.42227732
AGACCGA2600.031.39014225
Produced by FastQC (version 0.11.5)