FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184687
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT45650.38533384767453344No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40000.3376419256731947No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG38890.32827236223576356No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC36780.3104617506565025No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC33820.28547624815668615No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGATTGTTACTGGTTCTATATGAGCTGCAATAATAAT33500.2827751127513006No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32940.2780481257918758No Hit
TCTCTGGGCTCCAGCCCGAGGACGAGGCTGATTATTATTGCAGCTCATAT32630.27543140086790857No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGATTGTTACTGGTTCTATATGAGCTG31910.26935384620579106No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT31840.268762972835863No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30870.260575156138288No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29800.25154323462653005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28560.241076334930661No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26550.22410982816558297No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC25220.21288323413694923No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24450.20638362706774027No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23270.19642319026038102No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACAGCA22880.19313118148506736No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22770.19220266618946608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22740.1919494347452112No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22640.1911053299310282No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22620.19093650896819161No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22450.18950153078408052No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22310.18831978404422434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22020.1858718800830937No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21200.1789502206067932No Hit
GTTCTATATGAGCTGCAATAATAATCAGCCTCGTCCTCGGGCTGGAGCCCAGAGATGGTCAGGGAGGCCATGTTG21170.1786969891625383No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20950.17683995857133572No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20420.17236620305616587No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20330.1716065087234012No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19990.16873655235517904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19880.16780803705957775No Hit
GCCCAGAGATGGTCAGGGAGGCCATGTTGCCAGACTTGGAGCCAGAGAAG19840.16747039513390458No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19680.1661198274312118No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19240.16240576624880665No Hit
GGTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAGTCATCATTTTTGATGTCGATAAT19230.16232135576738835No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC18870.1592825784363296No Hit
ATATAGAACCAGTAACAATCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC18630.15725672688229042No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18170.1533738447370487No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGATTGTTACTGG18020.15210768751577422No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17950.1515168141458461No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16990.14341340792968946No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16740.14130314589423199No Hit
GATTATTATTGCAGCTCATATAGAACCAGTAACAATCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGT16430.1386864209702647No Hit
GCTTGGTCCCTCCGCCGAATACCAGATTGTTACTGGTTCTATATGAGCTG16400.13843318952600983No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16070.13564764363920598No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAAGTCATCATTTTTGATGTC16060.13556323315778768No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16010.13514118075069617No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15680.13235563486389232No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGATTGTTA15620.13184917197538254No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15440.13032978330985315No Hit
CCCCTGAGGGCCGATTATCGACATCAAAAATGATGACTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA15400.12999214138417994No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14950.12619366972035653No Hit
GTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA14750.12450546009199054No Hit
ATAATAATCAGCCTCGTCCTCGGGCTGGAGCCCAGAGATGGTCAGGGAGG14610.12332371335213436No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT14530.12264842950078798No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13920.11749939013427176No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13880.11716174820859855No Hit
GAGATGGTCAGGGAGGCCATGTTGCCAGACTTGGAGCCAGAGAAGCGATT13870.11707733772718025No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13850.11690851676434368No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13690.11555794906165089No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13590.11471384424746789No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13560.11446061280321301No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAAGTCATCATTTTTGATGTCG13500.1139541499147032No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13340.11260358221201043No Hit
CTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATGGTTATAA13340.11260358221201043No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13180.11125301450931764No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13130.11083096210222616No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13130.11083096210222616No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG13120.11074655162080786No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC13050.11015567825087977No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG13010.10981803632520658No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12920.10905834199244188No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12870.1086362895853504No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12860.1085518791039321No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC12760.10770777428974909No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12530.10576633321712824No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12520.10568192273570994No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12500.10551310177287333No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG12410.10475340744010865No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12400.10466899695869035No Hit
CAACAGCACCCAGGCAAAGCCCCCAAAGTCATCATTTTTGATGTCGATAATCGGCCCTCAGGGGTCTCTAATCG12320.10399371310734398No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12180.1028119663674878No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11880.10027965192493883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)