FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753806
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC72050.4108208091430865No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64460.3675435025310667No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58880.33572698462657785No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51320.29262073456243165No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46310.2640542910675411No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45420.2589796134806244No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44290.25253648351071895No Hit
GATCTATGCTGCAGCCACTTTGCAAAGTGGGATCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAC41340.23571592296981533No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41200.2349176590797386No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39110.22300071957787804No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTAACTT38960.2221454368385101No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38490.21946555092182374No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38210.21786902314167017No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36870.21022849733664953No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36630.20886004495366078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35810.2041844993117825No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35390.20178970764155213No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT35160.20047827410785457No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34160.1947763891787347No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA32710.1865086560315109No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32090.18297348737545657No Hit
GATTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATGTCAGGACC31060.1771005458984631No Hit
GTAGGAGACAGAGTCACCGTAACTTGCCGGGCAAGTCAGAGCATTAGCAGATATTTAAATTGGTATCAGCAAAAA30660.17481979192681515No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT30080.17151269866792565No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29980.17094251017501366No Hit
ATATTGTAGTGGTGACTGCTACTTTCTGGATGACTACTGGGGCCTGGGAACCCGGGTCACCGTCTCCTCAGCATC29470.16803454886116254No Hit
GTGCAGCCACAGTTCGTTTGAGATCCACCTTGGTCCCAGGGCCGAAAGTG29440.16786349231328893No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29040.16558273834164097No Hit
CTACAATATTGTCTCCTCGTCGCACAATAATAGACGGCCGTGTCCGCAGC29040.16558273834164097No Hit
ACTTACTACTGTCAACAGAATGTCAGGACCCCTTTCACTTTCGGCCCTGGGACCAAGGTGGATCTCAAACGAA28570.16290285242495464No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28100.1602229665082683No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27790.15845538218024116No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27200.15509127007206042No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT27070.15435002503127485No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26460.1508718752245117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGATCCACCTTGGTCCCAGGGCCGAAAGTGAAAGGGGTC26400.15052976212876454No Hit
CACCTGCACCGTCTCTGGTGCCCCCACCGAGACTTACTACTGGAGCTGGA26350.15024466788230853No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25930.14784987621207818No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25410.14488489604893587No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC25360.14459980180247986No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGAGA24980.1424330855294143No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24500.13969618076343676No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24440.1393540676676896No Hit
CTGCTACTCTGGCTCCGAGGGGGCAGATGTGACATCCAGATGACGCAGTC24370.1389549357226512No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA23890.13621803095667365No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGGCAGATGTGACAT23370.13325305079353134No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23360.13319603194424012No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23320.13296795654707533No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23210.13234074920487213No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23100.13171354186266895No Hit
CTCCTGATCTATGCTGCAGCCACTTTGCAAAGTGGGATCCCATCACGGTT23040.1313714287669218No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22510.12834942975448824No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22430.12789327896015865No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATGTCAGGACCCCTTTCACTTT22330.12732309046724666No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22300.12715203391937308No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22050.12572656268709312No Hit
GTGTAGGAGATATACGCAATCCACTCCAATCCCTTCCCTGGGGGCTGCCG21480.12247648827749477No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21130.12048082855230281No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20810.11865622537498446No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGCGTCATCTGGATGT20600.1174588295398693No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20520.11700267874553968No Hit
ATGCTGCAGCCACTTTGCAAAGTGGGATCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCA20520.11700267874553968No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20490.1168316221976661No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20370.11614739600617173No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20320.11586230175971574No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20270.11557720751325974No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20270.11557720751325974No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20220.11529211326680375No Hit
CTCCTACACTGGGAGTACCGACTACAATCCCTCCCTCAGGAGCCGAGCCA20210.11523509441751253No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20030.11420875513027096No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19940.11369558548665018No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19840.11312539699373819No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19690.11227011425437021No Hit
GTGGGATCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACC19440.11084464302209024No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19210.10953320948839268No Hit
GTCCCTCACCTGCACCGTCTCTGGTGCCCCCACCGAGACTTACTACTGGA19170.10930513409122787No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19160.10924811524193669No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19130.10907705869406309No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18820.10730947436603594No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18640.10628313507879433No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18480.10537083349013517No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18300.10434449420289359No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18190.10371728686069041No Hit
GGACCAAGGTGGATCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18150.1034892114635256No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATGTCAGGACCCCTTTCACTTTCGGCCCTGGGA18150.1034892114635256No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG18100.10320411721706961No Hit
CTGCTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCG18040.10286200412132243No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17800.10149355173833365No Hit
CAATAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGAGAACTGCTTCTTGGACGTGTCTA17680.10080932554683927No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCGTAACTTGCCGGGCAAGTCAGA17660.10069528784825688No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17610.10041019360180088No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGGCAGATGTGACATCCAGATGACGCAGTCTCCATCCTCC17590.10029615590321848No Hit
GGATTGGAGTGGATTGCGTATATCTCCTACACTGGGAGTACCGACTACAA17550.1000680805060537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6350.049.92885641
TCGTATG6550.048.29265240
ATGCCGT6850.046.62967744
ACGAGAC6850.046.5897122
GTATGCC6900.046.57113342
TATACAC6950.046.3802223
CTTATAC6850.046.078261
TTATACA6900.045.7430532
ATACACA7300.045.53644
TACACAT7400.044.9210475
CTCTCTA7000.044.80153329
CACGAGA7150.044.6272621
TCTCCGA7350.044.31170311
ATCTCCG7400.044.012310
CGAGACC7200.043.86961723
TCCGAGC7450.043.72189313
ATCTCGT7000.043.3701537
TGCTTGA7300.043.31912255
TCTACAT7150.043.06390432
CCGTCTT7400.043.0591647
Produced by FastQC (version 0.11.5)