FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753806
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67950.38744308093369506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63070.3596178824795901No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62710.35756520390510693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56600.3227266869881846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53780.30664737148806653No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46520.26525168690265627No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44120.2515671630727686No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42900.24461086345924235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39860.22727713327471794No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39250.22379898346795482No Hit
GATCTATGCTGCAGCCACTTTGCAAAGTGGGATCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAC38640.2203208336611917No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTAACTT38450.21923747552465897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37340.21290838325333591No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37250.2123952136097151No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35430.20201778303871692No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34630.19745627509542102No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33520.191127182824098No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32530.18548231674426932No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA32480.1851972224978133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32230.18377175126553336No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31620.18029360145877024No Hit
GATTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATGTCAGGACC31460.17938129987011106No Hit
GTAGGAGACAGAGTCACCGTAACTTGCCGGGCAAGTCAGAGCATTAGCAGATATTTAAATTGGTATCAGCAAAAA30460.1736794149409912No Hit
ATATTGTAGTGGTGACTGCTACTTTCTGGATGACTACTGGGGCCTGGGAACCCGGGTCACCGTCTCCTCAGCATC29060.1656967760402234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28920.1648985121501466No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28850.1644993802051082No Hit
CTACAATATTGTCTCCTCGTCGCACAATAATAGACGGCCGTGTCCGCAGC28570.16290285242495464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28520.16261775817849863No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT28010.1597097968646475No Hit
GTGCAGCCACAGTTCGTTTGAGATCCACCTTGGTCCCAGGGCCGAAAGTG27860.15885451412527954No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27620.15748606174229077No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26900.15338070459332448No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26840.15303859149757726No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC26650.1519552333610445No Hit
ACTTACTACTGTCAACAGAATGTCAGGACCCCTTTCACTTTCGGCCCTGGGACCAAGGTGGATCTCAAACGAA26640.15189821451175328No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26360.15030168673159972No Hit
GTGGGATCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACC26190.14933236629364938No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT26060.14859112125286375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25020.14266116092657913No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24930.14214799128295832No Hit
CTCCTGATCTATGCTGCAGCCACTTTGCAAAGTGGGATCCCATCACGGTT24670.14066550120138716No Hit
CTGCTACTCTGGCTCCGAGGGGGCAGATGTGACATCCAGATGACGCAGTC24580.14015233155776635No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24490.13963916191414558No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGATCCACCTTGGTCCCAGGGCCGAAAGTGAAAGGGGTC24390.1390689734212336No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24010.13690225714816803No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGAGA23480.1338802581357345No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA22720.1295468255896034No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGCGTCATCTGGATGT22580.12874856169952664No Hit
CACCTGCACCGTCTCTGGTGCCCCCACCGAGACTTACTACTGGAGCTGGA22550.12857750515165303No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22310.12720905276866426No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22280.12703799622079068No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22220.12669588312504346No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22110.12606867578284028No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21990.1253844495913459No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21970.12527041189276353No Hit
GTGTAGGAGATATACGCAATCCACTCCAATCCCTTCCCTGGGGGCTGCCG21880.12475724224914272No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGGCAGATGTGACAT21750.12401599720835714No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGTGATGGGATCCCA21740.12395897835906593No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21610.12321773331828034No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21520.12270456367465957No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21470.12241946942820357No Hit
CTCCTACACTGGGAGTACCGACTACAATCCCTCCCTCAGGAGCCGAGCCA21310.1215071678395444No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21140.12053784740159403No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21080.12019573430584682No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATGTCAGGACCCCTTTCACTTT21040.11996765890868204No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20290.11569124521184213No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20190.11512105671893014No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20020.11415173628097977No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19870.11329645354161179No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19560.11152886921358464No Hit
CTGCTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCG19290.10998936028272226No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18830.10736649321532712No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18500.10548487118871756No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCGTAACTTGCCGGGCAAGTCAGA18410.10497170154509677No Hit
GTCCCTCACCTGCACCGTCTCTGGTGCCCCCACCGAGACTTACTACTGGA18370.10474362614793199No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18050.10291902297061362No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATGTCAGGACCCCTTTCACTTTCGGCCCTGGGA18040.10286200412132243No Hit
GGATTGGAGTGGATTGCGTATATCTCCTACACTGGGAGTACCGACTACAA18000.10263392872415762No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17860.10183566483408085No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17630.1005242313003833No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17590.10029615590321848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.044.121761
GTATCAA28700.035.484171
CTTATAT450.003291719432.87015562
TATCAAC32500.031.3281062
ATCAACG33750.030.3645463
TCAACGC33900.030.031754
CAACGCA34050.029.8070435
TATACCT450.0053416429.7742565
GACAGGT2050.029.4145071
AACGCAG34800.029.0733746
CGCAGAG36100.028.0440068
AATGGTA7000.028.0000670
GCATGCG606.411643E-427.9388569
ACGCAGA36450.027.767597
AGAGTAC36350.027.40096511
GCAGAGT37700.026.8568749
GGTACGA500.00893965326.7937722
GTACGAC500.00894213826.7922443
GAGTTTA4600.026.217281
TCGTATA4350.025.39319261
Produced by FastQC (version 0.11.5)