FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991225
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTCTCTTAAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT62070.6261948598955838No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC55180.5566849100860047No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA37150.3747887714696461No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGAGGCGT36420.36742414688895053No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35360.3567303084567076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31580.3185956770662564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27150.27390350324093926No Hit
CAGTATAATAGTCTCTTAAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT25950.26179727105349443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22630.22830336200156373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21590.21781129410577818No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20550.2073192262099927No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20120.20298115967615826No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGC19830.20005548689752578No Hit
GTGTATTACTGTGCGAGCGGGGCGGGGGCTCAGGTGGGAGGGCGCTACTA19790.19965194582461093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19730.19904663421523874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19120.1928926328532876No Hit
GTCCTGATCTATGAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT18790.18956341900174026No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA18540.1870412872960226No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18460.18623420515019293No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG18030.18189613861635856No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17980.18139171227521503No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17600.17755807208252414No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17410.17564125198617872No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17410.17564125198617872No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG17280.17432974349920555No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17240.1739262024262907No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16760.16908370955131277No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16530.1667633483820525No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16160.16303059345759036No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15960.1610128880930162No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA15910.16050846175187267No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGA15880.16020580594718656No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14910.150419934929002No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC14540.14668718000453984No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14060.1418446871295619No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACAT13840.13962521122853036No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13830.13952432596030165No Hit
CTTAAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG13650.13770839113218492No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13610.1373048500592701No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13480.13599334157229692No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTA13250.13367298040303663No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13150.1326641277207496No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13020.1313526192337764No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12800.12913314333274484No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12730.1284269464551439No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCAGATGACCCAGTCTCCTTCCACC12520.12630835582234104No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12500.12610658528588364No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCCGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTCT12420.12529950314005397No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12280.12388710938485209No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12260.12368533884839467No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11830.11934727231456027No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG11690.11793487855935837No Hit
GATCTATGAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA11280.11379858256198139No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11150.1124870740750082No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA10980.11077202451512018No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10730.10824989280940252No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10560.1065348432495145No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10510.10603041690837095No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10490.10582864637191354No Hit
ACTTATTACTGCCAACAGTATAATAGTCTCTTAAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10430.1052233347625413No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG10400.10492067895785519No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10220.10310474412973845No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10150.10239854725213751No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10130.10219677671568009No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10120.10209589144745139No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10090.10179323564276527No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10090.10179323564276527No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA10040.10128880930162174No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10010.1009861534969356No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9970.10058261242402078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG750.055.14988340
CACGAGA2050.051.34226221
TACGGAT11100.050.9946170
ATCTCCG2100.050.1097210
CGTATGC1200.048.8882241
CGAGCCC2200.047.8295915
TACACAT2350.047.6678545
GTGGTAT2000.047.5234071
CGAGACT2300.047.26161223
ACGAGAC2250.046.79503322
CCCACGA2250.046.77142719
ATCTCGT900.045.76600337
CATCTCG1000.044.5718336
TCCGAGC2450.042.95118313
ACACATC2600.040.4732326
CCGAGCC2600.040.4711914
CTTATAC2800.038.7946171
TATACAC3000.038.471333
CGCGTAT451.01658334E-438.23585539
TCGCGTA451.0248774E-438.18313238
Produced by FastQC (version 0.11.5)