FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093114
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62130.5683762169362024No Hit
GCCTTGGGCTGACCAAGCACGGTCAGCCTGGTCCCCCCGCCGAAAATCCAAATATTCAGGCTGCTACGCCATGCT42630.3899867717365252No Hit
GTACTGGAACTGGATCCGGCAGGCCGCCGGCAACGGACTGGAGTGGATTGGGCGTATAAGTGGCAGAGGGGCGAT30840.28212976871579726No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26860.2457200255417093No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26230.23995667423525818No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24620.22522810978543867No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23220.21242066243776953No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21570.1973261709208738No Hit
GTCTCTGGTGACTCCCTCAACAATGATGGTCAGTACTGGAACTGGATCCG20090.18378686943905212No Hit
ATAGTAGACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGGGTCAGGGAGA20040.18332946060520677No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19730.18049352583536576No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19430.1777490728322938No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19370.1772001822316794No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19360.17710870046491034No Hit
GACCAAGCACGGTCAGCCTGGTCCCCCCGCCGAAAATCCAAATATTCAGG19030.1740898021615312No Hit
TGCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18440.16869237792215633No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18190.1664053337529297No Hit
GTAATAATCGGCTTCGTCCCCAGACTGGAGTCCGGTGATGGCCAGGGTGG17900.1637523625166268No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGCACGGTCAGCCTGGTCCCCC17860.16338643544955056No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17600.16100790951355484No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17440.15954420124524982No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17370.15890382887786636No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17060.15606789410802532No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16890.15451270407295123No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16750.15323195933818431No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGCTCTTCCTCCTCCTGGTGACAGCTCCCAGATGGGTC16350.14957268866742168No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15950.14591341799665908No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15800.1445411914951231No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15690.1435348920606634No Hit
GATTATTACTGCGGAGCATGGCGTAGCAGCCTGAATATTTGGATTTTCGG15320.14015006669020796No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15310.14005858492343892No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15220.13923524902251733No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15170.138777840188672No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15020.13740561368713602No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15000.13722265015359789No Hit
CACCTGCACTGTCTCTGGTGACTCCCTCAACAATGATGGTCAGTACTGGAACTGGATCCGGCAGGCCGCCGGCAA14970.13694820485329068No Hit
GGCCATCACCGGACTCCAGTCTGGGGACGAAGCCGATTATTACTGCGGAG14810.13548449658498565No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14720.13466116068406406No Hit
GTCTACTATTGTGCGAGAAGTCTGAAGAAAGATTGTTCTGATGGTGTTTG14450.1321911529812993No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14120.12917225467792015No Hit
CCCTCAGTGTCTGCGGCCCCCGGACAGACGGTCACCATCTCCTGCTCTGG14000.12807447347669138No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13730.12560446577392662No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13580.12423223927239063No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13530.12377483043854529No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCCGGACAGACGGTCACCATCTCCTG13370.12231112217024026No Hit
GACTTGGAGGCAGAGAATCGGTCAGGAATCCCTGAGGGGCGGTAAATATT13300.12167074980285679No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13200.12075593213516614No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13090.11974963270070642No Hit
GTGCTGGTACCAGGAGACAGAATCACTCCCAATGTTGGAGCTGGTTCCAG13080.11965815093393736No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13010.1190177785665539No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12970.11865185149947763No Hit
GGCTGACCGTGCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAG12800.11709666146440353No Hit
GTACCAGCACCTCCCAGGAAAAGCCCCCAAACTCCTCATTTTTGACAATATTTACCGCCCCTCAGGGATTCCTGA12760.11673073439732726No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT12590.11517554436225316No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12570.11499258082871502No Hit
GGCCAGACTTGGAGGCAGAGAATCGGTCAGGAATCCCTGAGGGGCGGTAA12510.11444369022810064No Hit
GCGTAGCAGCCTGAATATTTGGATTTTCGGCGGGGGGACCAGGCTGACCGTGCTTGGTCAGCCCAAGGCTGCCCC12250.11206516429210493No Hit
GTGATGGCCAGGGTGGCTGACGGGCCAGACTTGGAGGCAGAGAATCGGTC12230.11188220075856682No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12230.11188220075856682No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12220.11179071899179774No Hit
AGTCTGGCCCGTCAGCCACCCTGGCCATCACCGGACTCCAGTCTGGGGAC12200.11160775545825963No Hit
CGGTCAGCCTGGTCCCCCCGCCGAAAATCCAAATATTCAGGCTGCTACGCCATGCTCCGCAGTAATAATCGGCTT12060.1103270107234927No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11820.10813144832103513No Hit
CAATAGTAGACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGGGTCAGGGA11820.10813144832103513No Hit
GCCTGGTCCCCCCGCCGAAAATCCAAATATTCAGGCTGCTACGCCATGCTCCGCAGTAATAATCGGCTTCGTCCC11770.1076740394871898No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11590.10602736768534664No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11370.10401476881642721No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11270.10309995114873653No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11270.10309995114873653No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11190.10236809701458403No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGCACGGTCAGCCTGGTCCCCCCGCCGAAAAT11150.10200216994750776No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11070.10127031581335524No Hit
CCTCAGGGATTCCTGACCGATTCTCTGCCTCCAAGTCTGGCCCGTCAGCCACCCTGGCCATCACCGGACTCCAGT11050.1010873522798171No Hit
CTGCTACGCCATGCTCCGCAGTAATAATCGGCTTCGTCCCCAGACTGGAG11000.10062994344597177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)