FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093114
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59080.5404742780716376No Hit
GCCTTGGGCTGACCAAGCACGGTCAGCCTGGTCCCCCCGCCGAAAATCCAAATATTCAGGCTGCTACGCCATGCT38240.3498262761249055No Hit
GTACTGGAACTGGATCCGGCAGGCCGCCGGCAACGGACTGGAGTGGATTGGGCGTATAAGTGGCAGAGGGGCGAT27810.25441079338477046No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27480.2513918950813913No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27270.24947077797924094No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCCGGACAGACGGTCACCATCTCCTG25880.23675481239834087No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23610.2159884513417631No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23260.21278658950484577No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21990.2011684051251745No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCCGGACAGACGGTCAC20940.19156281961442265No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20250.18525057770735714No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19920.182231679403978No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19600.1793042628673679No Hit
ATAGTAGACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGGGTCAGGGAGA19180.17546202866306715No Hit
GTCTCTGGTGACTCCCTCAACAATGATGGTCAGTACTGGAACTGGATCCG19160.17527906512952904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18510.16933275028953979No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17900.1637523625166268No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGCTCTTCCTCCTCCTGGTGACAGCTCCCAGATGGGTC17640.16137383658063112No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17620.16119087304709298No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17530.16036753714617138No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16570.15158528753634112No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16560.15149380576957205No Hit
TGCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16290.1490237980668073No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16260.1487493527665001No Hit
GGCTGACCGTGCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAG14860.13594190541883097No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14810.13548449658498565No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14790.13530153305144751No Hit
GATTATTACTGCGGAGCATGGCGTAGCAGCCTGAATATTTGGATTTTCGG14770.1351185695179094No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14730.13475264245083313No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14720.13466116068406406No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14470.13237411651483744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14380.13155078061391584No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGCACGGTCAGCCTGGTCCCCC14330.13109337178007052No Hit
GTAATAATCGGCTTCGTCCCCAGACTGGAGTCCGGTGATGGCCAGGGTGG14220.1300870723456108No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14120.12917225467792015No Hit
GACCAAGCACGGTCAGCCTGGTCCCCCCGCCGAAAATCCAAATATTCAGG14050.12853188231053667No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13900.1271596558090007No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGCACGGTCAGCCTGGTCCCCCCGCCGAAAAT13830.12651928344161725No Hit
GGCCATCACCGGACTCCAGTCTGGGGACGAAGCCGATTATTACTGCGGAG13770.12597039284100287No Hit
CCCTCAGTGTCTGCGGCCCCCGGACAGACGGTCACCATCTCCTGCTCTGG13760.1258789110742338No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13680.12514705694008127No Hit
CACCTGCACTGTCTCTGGTGACTCCCTCAACAATGATGGTCAGTACTGGAACTGGATCCGGCAGGCCGCCGGCAA13640.12478112987300502No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13540.12386631220531436No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13470.1232259398379309No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13380.12240260393700932No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13330.12194519510316398No Hit
GTCTACTATTGTGCGAGAAGTCTGAAGAAAGATTGTTCTGATGGTGTTTG13070.1195666691671683No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13050.11938370563363015No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13040.1192922238668611No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12880.11782851559855605No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT12720.11636480733025101No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12690.11609036202994381No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12610.11535850789579129No Hit
GCGTAGCAGCCTGAATATTTGGATTTTCGGCGGGGGGACCAGGCTGACCGTGCTTGGTCAGCCCAAGGCTGCCCC12510.11444369022810064No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12360.11307146372656467No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12280.11233960959241213No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12130.11096738309087616No Hit
CAATAGTAGACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGGGTCAGGGA12030.1100525654231855No Hit
GTGCTGGTACCAGGAGACAGAATCACTCCCAATGTTGGAGCTGGTTCCAG12000.10977812012287831No Hit
GTACCAGCACCTCCCAGGAAAAGCCCCCAAACTCCTCATTTTTGACAATATTTACCGCCCCTCAGGGATTCCTGA11780.10776552125395886No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT11440.10465514118381065No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11290.10328291468227467No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT11260.10300846938196748No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG11150.10200216994750776No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11140.1019106881807387No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT11130.10181920641396963No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11080.1013617975801243No Hit
CTCCTGCTCTGGAACCAGCTCCAACATTGGGAGTGATTCTGTCTCCTGGTACCAGCACCTCCCAGGAAAAGCCCC11000.10062994344597177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG100.0076415143141.4111570
GGTATCA9350.044.5214731
GTGGTAT3250.042.6949541
TGGTATC3300.039.9877852
AATGGTA4050.038.40796770
GTATCAA20300.033.8435631
GTGTAGG1209.640644E-1131.023261
CAGTATA450.00507794930.0831661
TATCAAC23150.029.3779682
TGCTAAA1000.00686246928.28222870
TAGGCAA1255.9639587E-428.28222870
GACAGGT1353.8380676E-1027.5762351
ACTCGTA2750.027.44797359
ATCAACG24900.027.3123
TCGTATA2800.027.12523861
TCAACGC25100.027.0931384
CCCTAAT500.00847765427.08600212
TCTAGGC500.00850608627.0674173
AACGCAG25300.026.883886
CAACGCA25400.026.7755895
Produced by FastQC (version 0.11.5)