FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569522
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17650.30990901141659144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15430.2709289544565443No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14150.2484539666597603No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13500.2370408869192059No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13470.2365141293927188No Hit
GTCTGGGACAGACTTCACTCTCACCATTAGCAGCCTAGAGCCTGAAGATT11730.20596219285646558No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG11500.201923718486731No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10820.18998388121968948No Hit
GCCTAGAGCCTGAAGATTTTGCTGATTATTACTGTCAGCAGCGTAGCAAC10340.1815557607958955No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10160.17839521563697275No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT10000.17558584216237474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9820.17242529700345202No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9500.16680655005425604No Hit
GGCCAAAAGTGTACGGAGACCTGTTGCTACGCTGCTGACAGTAATAATCAGCAAAATCTTCAGGCTCTAGGCTG9390.1648751057904699No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9360.16434834826398276No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9300.16329483321100852No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTTGCCTGGTA9250.16241690400019665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9200.16153897478938478No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9150.16066104557857291No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTTGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT8950.15714932873532542No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8930.15679815705100067No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8910.15644698536667592No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8780.15416436941856504No Hit
GACTTCACTCTCACCATTAGCAGCCTAGAGCCTGAAGATTTTGCTGATTA8710.15293526852342842No Hit
GAATACAGGAGACTCACCATGCAGTTTGGGCTGAGCTGGGTTTTCCTTGT8560.1503014808909928No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGAC8450.1483700366272067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8290.14556066315260868No Hit
GCTATGCACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG7970.13994191620341268No Hit
TTATTACTGTCAGCAGCGTAGCAACAGGTCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA7930.13923957283476318No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7780.13660578520232758No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7620.13379641172772955No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGACCTGTTGCTACGCTG7450.1308114524109692No Hit
GTCAGAGTGTTAGCAGCTACTTTGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATG7340.12888000814718306No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7290.1280020789363712No Hit
CAGTAATAATCAGCAAAATCTTCAGGCTCTAGGCTGCTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6950.12203216030285047No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6780.11904720098609009No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT6780.11904720098609009No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6690.11746692840662872No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6500.11413079740554359No Hit
GATTATTACTGTCAGCAGCGTAGCAACAGGTCTCCGTACACTTTTGGCCA6490.11395521156338123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6470.11360403987905647No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6450.11325286819473172No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6390.11219935314175748No Hit
GTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGC6380.1120237672995951No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT6360.11167259561527035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6300.11061908056229611No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6250.10974115135148424No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6230.10938997966715948No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6230.10938997966715948No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6130.10763412124553573No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6100.1071073637190486No Hit
CACCATTAGCAGCCTAGAGCCTGAAGATTTTGCTGATTATTACTGTCAGCAGCGTAGCAACAGGTCTCCGTACA6090.10693177787688624No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6040.10605384866607435No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATTAGCAG5980.10500033361310011No Hit
GCGTAGCAACAGGTCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT5870.10306888934931398No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5800.10183978845417736No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5720.10043510171687836No Hit
GTGTTAGCAGCTACTTTGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC5700.10008393003255363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)