FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569522
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17340.3044658503095578No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12620.22158933280891696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12470.21895554517648133No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11730.20596219285646558No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11530.2024504760132181No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10980.19279325469428749No Hit
GTCTGGGACAGACTTCACTCTCACCATTAGCAGCCTAGAGCCTGAAGATT10890.19121298211482612No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10640.18682333606076673No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10230.17962431653210936No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9630.1690891660023669No Hit
GCCTAGAGCCTGAAGATTTTGCTGATTATTACTGTCAGCAGCGTAGCAAC9620.16891358016020452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9030.1585540154726244No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8940.15697374289316304No Hit
GGCCAAAAGTGTACGGAGACCTGTTGCTACGCTGCTGACAGTAATAATCAGCAAAATCTTCAGGCTCTAGGCTG8900.15627139952451352No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTTGCCTGGTA8500.14924796583801855No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8470.14872120831153143No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8460.14854562246936906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8400.14749210741639482No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTTGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT8230.14450714809963444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7950.13959074451908796No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7890.1385372294661137No Hit
GACTTCACTCTCACCATTAGCAGCCTAGAGCCTGAAGATTTTGCTGATTA7790.13678137104448992No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGAC7780.13660578520232758No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7680.13484992678070382No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7530.1322161391482682No Hit
GAATACAGGAGACTCACCATGCAGTTTGGGCTGAGCTGGGTTTTCCTTGT7510.13186496746394344No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGACCTGTTGCTACGCTG7480.13133820993745632No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7240.12712414972555933No Hit
GCTATGCACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG7220.12677297804123458No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7120.12501711961961084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6960.12220774614501284No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6950.12203216030285047No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6870.12062747356555147No Hit
TTATTACTGTCAGCAGCGTAGCAACAGGTCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA6790.11922278682825246No Hit
GTCAGAGTGTTAGCAGCTACTTTGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATG6710.11781810009095348No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6280.11026790887797135No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC6250.10974115135148424No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6140.1078097070876981No Hit
CAGTAATAATCAGCAAAATCTTCAGGCTCTAGGCTGCTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6130.10763412124553573No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6020.10570267698174962No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT5970.10482474777093773No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5910.10377123271796349No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5900.10359564687580111No Hit
GATTATTACTGTCAGCAGCGTAGCAACAGGTCTCCGTACACTTTTGGCCA5830.10236654598066448No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5790.10166420261201498No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5770.10131303092769024No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5750.1009618592433655No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5740.10078627340120311No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5730.10061068755904073No Hit
GTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGC5700.10008393003255363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGA200.006866632552.66069853
CTAGGTA200.00704580152.3188449
TCGCTAT200.00782731150.9454113
AACGACC305.6706945E-447.0057455
GCATTAC405.449028E-542.4992871
GACAGGT903.6379788E-1241.5548551
GGTATCA5500.038.94481
CCGATCA800.002143261335.8655270
GGTTTAG400.00280248533.96360812
GTATCAA11950.033.8571741
GAGTTTA2250.031.73281
CGGGTAC450.0049866630.1925246
CATCTAC500.00626449128.81530863
TATCAAC13800.028.5666792
AGTTTAT2500.028.5469762
TGTTAGA6050.028.4552970
ATCAACG13800.028.0618213
TCAACGC13900.027.613134
CGGGTGC500.0083423827.1732716
CAACGCA14200.027.0297555
Produced by FastQC (version 0.11.5)