FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956096
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAATTGGTTGTTGGACGTGTCTACGGA40370.42223793426601514No Hit
GTATTACTGTCAGCAGTATGTTACCTCTCCGTTCGATTTTGGCCAGGGGA38420.40184249280406986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32690.3419112725082No Hit
GTGTATTACTGTGCGGCAGTGCATAGCACTGGCTGGGGACCTGAATACTT32050.3352173840283821No Hit
ATCTATTATAGTGGAACCACCTACTACAACCCGTCCCTCAAGAGTCGAGT28250.29547242117946315No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCAGGAGTAATAGTTACTTCTGGG26280.2748677957025236No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25830.2701611553651516No Hit
GTGTCTGGTGGCTCCATCAGGAGTAATAGTTACTTCTGGGGCTGGATCCG25430.26597747506526537No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25200.26357185889283086No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA23830.2492427538657206No Hit
GTATGTTACCTCTCCGTTCGATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23770.24861520182073768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20270.21200799919673338No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19100.19977073431956624No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTGTCAGTAG18910.1977834861771203No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18390.19234470178726823No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18050.18878857353236494No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18030.18857938951737063No Hit
CTCCTGCAGGGCCAGTCGGAGTGTCAGTAGCAGCGACTTAGCCTGGTACC17760.18575540531494747No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG17720.18533703728495882No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT17710.1852324452774617No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17640.1845003012249816No Hit
GCATAGCACTGGCTGGGGACCTGAATACTTCCAGAAGTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCGGCATC16360.17111252426534573No Hit
GTGTATTACTGTCAGCAGTATGTTACCTCTCCGTTCGATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16360.17111252426534573No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16350.17100793225784858No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16250.16996201218287704No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16050.16787017203293392No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16030.16766098801793963No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA15900.1663012919204766No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15480.1619084276055961No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15460.16169924359060178No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15230.15929362741816722No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15140.1583522993506928No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14930.15615586719325256No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14740.15416861905080662No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14620.15291351496084074No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14580.15249514693085214No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCCAACAACCAATTCTCCCTGAGG14540.1520767789008635No Hit
GGTAACATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT14490.15155381886337774No Hit
ATAATAGATAGTCCCAATCCACTCTAGCCCCTTCCCCGGGGGCTGGCGGATCCAGCCCCAGAAGTAACTATTACT14460.15124004284088627No Hit
GTATTACTGTGCGGCAGTGCATAGCACTGGCTGGGGACCTGAATACTTCCAGAAGTGGGGCCAGGGCACCCTGGT14430.1509262668183948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14330.14988034674342327No Hit
GTGCTATGCACTGCCGCACAGTAATACACAGCCGTGTCTGCGGCGGTCAC14330.14988034674342327No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14210.1486252426534574No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATCG14090.14737013856349152No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13720.1435002342860968No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13620.14245431421112523No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13460.14078084209117075No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12770.13356399357386706No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12540.1311583774014325No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12520.13094919338643818No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12460.13032164134145524No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12300.12864816922150077No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12270.1283343931990093No Hit
GTCCGTAGACACGTCCAACAACCAATTCTCCCTGAGGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTA11740.12279101680166009No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT11640.12174509672668854No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGA11620.12153591271169423No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA11590.12122213668920274No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT11520.12048999263672268No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTCGCTGCTACTGACACTCCGA11480.12007162460673404No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT11300.11818896847178526No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA11290.11808437646428811No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11140.11651549635183077No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11120.11630631233683647No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11100.11609712832184216No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT11100.11609712832184216No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11080.11588794430684785No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11030.11536498426936206No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10970.11473743222437914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10950.11452824820938484No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10940.11442365620188769No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACCAGGGTGCCCTGGCCCCACTTCTGGAAGTATTCAGGTC10860.11358692014191044No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGTTACCTCT10510.10992619987951001No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCC10240.10710221567708682No Hit
CTATTATAGTGGAACCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCCAACAA10170.10637007162460674No Hit
ATGTTACCTCTCCGTTCGATTTTGGCCAGGGGACCAAGCTGGAGATCAAA10120.10584711158712096No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9960.10417363946716648No Hit
TCGCATGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGA9960.10417363946716648No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9950.10406904745966933No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG9920.10375527143717785No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9800.10250016734721198No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTCTGTTGACGCAGTC9740.10187261530222906No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9710.1015588392797376No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9580.10019914318227457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC800.055.44500427
TACATCG200.00740682651.66462339
CGTATGC900.049.8698341
TCGTATG850.048.6718740
GGTATCA6300.046.4839481
GACCGTA1000.044.3258126
GTACTAG1050.042.29034830
CGTACTA1050.042.2748329
CTAGATC1100.040.43594433
ATGCCGT1150.039.17605244
TATGCCG1150.039.1281543
CCGAAAC400.001451447538.8375868
GTATCAA16100.038.0707781
CCGTCTT1200.037.7224147
TACTAGA1200.037.0176531
ACGAGAC1200.036.90145522
CGTCTTC1250.036.27170648
TAGATCT1151.8189894E-1235.7364434
AGACCGT1250.035.4495125
GTATGCC1400.034.5619242
Produced by FastQC (version 0.11.5)