FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956096
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAATTGGTTGTTGGACGTGTCTACGGA40100.41941395006359194No Hit
GTATTACTGTCAGCAGTATGTTACCTCTCCGTTCGATTTTGGCCAGGGGA35530.37161540263739207No Hit
GTGTATTACTGTGCGGCAGTGCATAGCACTGGCTGGGGACCTGAATACTT33200.3472454648905549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32020.33490360800589064No Hit
ATCTATTATAGTGGAACCACCTACTACAACCCGTCCCTCAAGAGTCGAGT26920.2815616841823415No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCAGGAGTAATAGTTACTTCTGGG25010.2615846107503849No Hit
GTGTCTGGTGGCTCCATCAGGAGTAATAGTTACTTCTGGGGCTGGATCCG24610.2574009304504987No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23740.2483014257982462No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA22730.23773763304103354No Hit
GTATGTTACCTCTCCGTTCGATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22640.23679630497355914No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21780.2278013923288038No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20190.21117126313675616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19630.20531411071691547No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC19500.20395441461945243No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18960.19830644621460608No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTGTCAGTAG17830.18648754936742754No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16990.17770182073766652No Hit
CTCCTGCAGGGCCAGTCGGAGTGTCAGTAGCAGCGACTTAGCCTGGTACC16900.17676049267019212No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16830.17602834861771202No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16810.1758191646027177No Hit
GTGTATTACTGTCAGCAGTATGTTACCTCTCCGTTCGATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16750.17519161255773477No Hit
GTGCTATGCACTGCCGCACAGTAATACACAGCCGTGTCTGCGGCGGTCAC15730.16452322779302497No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15520.1623267956355847No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15440.16149005957560747No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15260.15960740344065869No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15130.15824770734319565No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14690.15364565901332083No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14600.15270433094584643No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14450.1511354508333891No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14310.14967116272842892No Hit
GCATAGCACTGGCTGGGGACCTGAATACTTCCAGAAGTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCGGCATC14230.1488344266684517No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCCAACAACCAATTCTCCCTGAGG14230.1488344266684517No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA14150.14799769060847445No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14120.147683914585983No Hit
GTATTACTGTGCGGCAGTGCATAGCACTGGCTGGGGACCTGAATACTTCCAGAAGTGGGGCCAGGGCACCCTGGT14080.14726554655599436No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13960.1460104424660285No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATCG13800.14433697034607404No Hit
ATAATAGATAGTCCCAATCCACTCTAGCCCCTTCCCCGGGGGCTGGCGGATCCAGCCCCAGAAGTAACTATTACT13790.14423237833857688No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13570.14193135417363947No Hit
GGTAACATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT13240.13847981792623335No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13190.1379568578887476No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13010.13607420175379878No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12940.13534205770131869No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12260.12822980119151214No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12240.12802061717651783No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12150.12707928910904345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12010.12561500100408327No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11970.12519663297409467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11750.12289560880915724No Hit
GTCCGTAGACACGTCCAACAACCAATTCTCCCTGAGGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTA11520.12048999263672268No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11380.1190257045317625No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGA11380.1190257045317625No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG11330.11850274449427672No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11220.11735223241180802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11060.11567876029185355No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11050.11557416828435639No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT11050.11557416828435639No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10980.1148420242318763No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10930.11431906419439053No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10930.11431906419439053No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10890.1139006961644019No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10760.11254100006693889No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT10520.11003079188700716No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT10340.10814813575205837No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACCAGGGTGCCCTGGCCCCACTTCTGGAAGTATTCAGGTC10280.10752058370707544No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10210.10678843965459535No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9990.10448741548965794No Hit
TCGCATGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGA9910.10365067942968072No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTCTGTTGACGCAGTC9900.10354608742218355No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA9890.10344149541468639No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9850.10302312738469778No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9840.10291853537720062No Hit
CACCTGCACTGCTGCCCACCCCGAGTTGAAGACCCCACTAACCGCCAACA9810.10260475935470914No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9780.10229098333221769No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTCGCTGCTACTGACACTCCGA9770.10218639132472053No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9740.10187261530222906No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT9730.10176802329473192No Hit
GGGCTAGAGTGGATTGGGACTATCTATTATAGTGGAACCACCTACTACAA9670.10114047124974898No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGTTACCTCT9630.10072210321976036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)