FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320258
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52190.395301524399019No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40940.3100909064743406No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39630.3001686034093336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36920.27964231233592224No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31820.2410134988767347No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31420.23798378801719058No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30660.23222733738405676No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTCCTGATCTATTTGGGTT30570.23154565244065933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30180.2285916843526038No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC29280.22177483491862957No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27430.20776242219323798No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26220.19859754684311703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24610.18640296063345194No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24380.18466087688921407No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24310.18413067748879386No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24310.18413067748879386No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24210.18337324977390781No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGCGTCCCTTGGGCGAAGGTG24060.18223710820157876No Hit
GTAATAAATCCCAACGTCCTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCA23350.17685937142588795No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGCGTCCCTTGGGCGAAGGTGGGAGGAGTTTG23190.1756474870820703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23150.17534451599611592No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22800.1726935189940148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22750.1723148051365718No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22300.16890638041958467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22270.16867915210511886No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22210.16822469547618724No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21490.1627712159290078No Hit
GTCAGGGACCCCGGGGGCCCGATTAGAACCCAAATAGATCAGGAACTGTGGAGACTGCCCTGGCTTCTGCAGGTA21420.1622410165285876No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAA21370.16186230267114457No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20700.15678753698140818No Hit
GAGTTTGCAGAGCTTGCATGCAGTAATAAATCCCAACGTCCTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAAT20600.15603010926652214No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20300.15375782612186406No Hit
AGCCAGGGCAGTCTCCACAGTTCCTGATCTATTTGGGTTCTAATCGGGCC20040.15178851406316038No Hit
CTGCAAACTCCTCCCACCTTCGCCCAAGGGACGCGACTGGAGATTAAACG19420.147092462230867No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAAAATCAGCAGAG19150.1450474074006747No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC19040.14421423691430008No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18770.14216918208410778No Hit
GTTCCTGATCTATTTGGGTTCTAATCGGGCCCCCGGGGTCCCTGACAGGT18700.14163898268368758No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18650.14126026882624457No Hit
GTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTGCAAACTCCTCCCACCTTCGCCCAAGGGACG18320.13876075736712068No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18070.1368671880799056No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC18020.13648847422246257No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17900.13557956096459933No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA17690.1339889627633387No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17660.13376173444887288No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17040.1290656826165795No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16900.12800528381573906No Hit
GTCTCCACAGTTCCTGATCTATTTGGGTTCTAATCGGGCCCCCGGGGTCC16770.1270206277863872No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16620.12588448621405818No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16560.12543002958512656No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA16250.12308200366897984No Hit
GAGTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTGCAAACTCCTCCCACCTTCGCCCAAGGGA16010.12126417715325338No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT15880.12027952112390154No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15760.1193706078660383No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15650.11853743737966367No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC15500.11740129580733462No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15160.11482604157672213No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15060.1140686138618361No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTCCTGAT15010.11368990000439308No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14870.11262950120355264No Hit
GATTTTACACTAAAAATCAGCAGAGTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTGCAAACT14640.11088741745931478No Hit
CAGTAATAAATCCCAACGTCCTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCA14600.11058444637336035No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCT14500.10982701865847433No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC14430.10929681925805411No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14330.10853939154316808No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14220.10770622105679344No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC14210.10763047828530484No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14170.10732750719935043No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14170.10732750719935043No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13860.10497948128320372No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13810.10460076742576073No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13780.10437353911129492No Hit
GCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCC13760.10422205356831771No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTCCTGATCTATTTGGGTTCTAAT13600.10301016922450006No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC13520.10240422705259124No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13430.1017225421091938No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT13340.10104085716579637No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13330.10096511439430778No Hit
GGTGGGAGGAGTTTGCAGAGCTTGCATGCAGTAATAAATCCCAACGTCCT13260.10043491499388757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAG200.00663127453.1311653
GGTATCA13600.039.533941
GTGGTAT4300.036.9607961
ATAAACG601.5535059E-533.772923
TTCTCGG850.00296082733.58540770
GTATCAA30400.033.0365561
TGGTATC5050.030.778612
TATCAAC34750.028.6846662
ATCAACG34800.028.6293433
TCAACGC35150.028.4349674
CAACGCA35700.027.9968955
TTGCGGA500.00751185127.76624543
AACGCAG36050.027.725086
GTACGTA650.00785921227.50962469
CGCAGAG38300.026.537148
ACGCAGA38750.025.8804077
TGTTAGA17150.025.80103370
AGAGTAC39050.025.59414311
CAGAGTA39850.025.50495110
TATAAAC801.1015349E-425.3421732
Produced by FastQC (version 0.11.5)