FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882743
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29540.33463873403697336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27570.312321932884203No Hit
GTATGGTAGTTCATCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT24110.2731259267986265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22330.2529615074829254No Hit
CTTCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA21900.24809032753587398No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21510.24367228060715296No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAGGATT21310.2414066155155011No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20710.23460962024054569No Hit
GTATCACTGTCAGCAGTATGGTAGTTCATCCCTCACCTTCGGCCAAGGGA20040.22701964218351206No Hit
CTACCATACTGCTGACAGTGATACACTGCAAAATCCTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19840.22475397709186026No Hit
CCATAGAAGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTATCTACTGCCAACACTCTGA19290.21852339808981777No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19190.21739056554399183No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT19020.2154647502160878No Hit
CAGTGATACACTGCAAAATCCTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA18710.2119529693240275No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18130.20538254055823724No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16950.19201511651749148No Hit
GTGTATCACTGTCAGCAGTATGGTAGTTCATCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16720.18940960166209192No Hit
ATATTACTGTGCGAGCGTGCTGTCACCTAACTTCCTAAAGACACAGTTCTACTTTGACAACTGGGGCCAGGGAAC16600.1880502026071008No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16210.1836321556783798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16000.18125320733214537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15500.17558904460301583No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15350.17388979578427696No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGTAGATACTTAGCCTGGTACC15070.17071786465596442No Hit
GTGATACACTGCAAAATCCTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA14880.1685654828188952No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGACTATTCACTTGCAAATACA14700.16652638423640856No Hit
CTATATTACTGTGCGAGCGTGCTGTCACCTAACTTCCTAAAGACACAGTT14630.1657334014543304No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14510.16437400239933933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14330.1623349038168527No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14180.16063565499811383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14080.15950282245228792No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14070.15938953919770535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13880.15723715736063612No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13320.150893295104011No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12820.14522913237488147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12580.14251033426489929No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG12270.13899855337283898No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12180.13797900408159566No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12140.1375258710632653No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11870.13446722318953536No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCA11870.13446722318953536No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11640.13186170833413574No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11570.13106872555205762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11530.13061559253372726No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11400.12914291022415358No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAGGATTTTGCAGTGTA11230.12721709489624955No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC11050.1251779963137629No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10870.12313889773127626No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10740.12166621542170257No Hit
GGACTGAACAGAGAGAACTCACTATGGAGTTTGGGCTGAGCTGGCTTTTT10660.12075994938504186No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10500.1189474173117204No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10480.1187208508025552No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10410.11792786802047707No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10300.11668175222006857No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10250.11611533594715563No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10190.11543563641966009No Hit
GCCCTATATTACTGTGCGAGCGTGCTGTCACCTAACTTCCTAAAGACACA10160.1150957866559123No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9910.11226370529134753No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9820.1112441560001042No Hit
GTCAGAGTGTTGGCAGTAGATACTTAGCCTGGTACCAGCAGAAGCCTGGC9740.1103378899634435No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9630.10909177416303499No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9620.10897849090845241No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9560.10829879138095685No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9400.1064862593076354No Hit
CCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCACCATAGAAGAGGAGCCTGGGAGCCTGGCCAGG9390.1063729760530528No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9320.10557999327097467No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9230.10456044397973137No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGACTATTCACTTGCAAATA9210.10433387747056617No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9150.10365417794307064No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9110.10320104492474026No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8920.10104866308767103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA200.00695952952.48450547
GGTATCA4750.045.9464951
CTTATAC2500.038.948491
GCCGTCT2600.037.60791446
TCGTATG2500.037.1462740
ATGCCGT2650.036.72951544
CCCACGA2500.036.2603319
GTATCAA11950.035.9645581
CGTATGC2600.035.8402941
ATGCATG800.002615037634.44466870
TATACAC2850.034.1653443
CGAGGCT2800.033.7003929
AGACCGA2800.033.60674725
TTATACA2900.033.5762832
GTATGCC3000.033.4325342
ACGAGAC2850.032.99096722
ATCTCGT2350.031.9581837
CACGAGA2850.031.80911321
TCTCCGA2850.031.80550811
ACCGAGG3000.031.41439427
Produced by FastQC (version 0.11.5)