FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882743
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29990.33973648049319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28020.3174196793404196No Hit
GTATGGTAGTTCATCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT24680.27958307230983426No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAGGATT22050.24978957635461282No Hit
CTTCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA21660.2453715294258918No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21620.2449183964075614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21340.24174646527924887No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21120.23925423367843188No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20390.23098455609390275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18780.2127459521061056No Hit
GTATCACTGTCAGCAGTATGGTAGTTCATCCCTCACCTTCGGCCAAGGGA18670.2114998363056971No Hit
CTACCATACTGCTGACAGTGATACACTGCAAAATCCTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC18640.21115998654194934No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT18080.20481612428532428No Hit
CCATAGAAGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTATCTACTGCCAACACTCTGA18010.20402314150324616No Hit
CAGTGATACACTGCAAAATCCTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA17350.19654644670079516No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17150.1942807816091433No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17030.19292138255415223No Hit
GTGTATCACTGTCAGCAGTATGGTAGTTCATCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16790.19020258444417004No Hit
ATATTACTGTGCGAGCGTGCTGTCACCTAACTTCCTAAAGACACAGTTCTACTTTGACAACTGGGGCCAGGGAAC16190.1834055891692146No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16180.183292305914632No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15390.17434292880260735No Hit
GTGATACACTGCAAAATCCTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA14650.1659599679634956No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGTAGATACTTAGCCTGGTACC14650.1659599679634956No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14250.16142863778019198No Hit
CTATATTACTGTGCGAGCGTGCTGTCACCTAACTTCCTAAAGACACAGTT13770.1559910415602276No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13740.15565119179647985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13730.15553790854189725No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13530.15327224345024543No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13500.15293239368649766No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13440.1522526941590021No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG13390.15168627788608915No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGACTATTCACTTGCAAATACA13370.151459711376924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13310.15078001184942844No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12910.1462486816661248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12520.14183063473740376No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12260.13888527011825638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12170.13786572082701307No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCA11820.1339008069166224No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11750.13310782413454425No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11670.13220155809788353No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11290.12789679442374507No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11200.12687724513250176No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAGGATTTTGCAGTGTA10850.1229123312221111No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10850.1229123312221111No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10730.12155293216711997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC10570.11974040009379854No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10450.11838100103880744No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10450.11838100103880744No Hit
GCCCTATATTACTGTGCGAGCGTGCTGTCACCTAACTTCCTAAAGACACA10340.11713488523839893No Hit
GGACTGAACAGAGAGAACTCACTATGGAGTTTGGGCTGAGCTGGCTTTTT10230.11588876943799044No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10210.11566220292882526No Hit
GTCAGAGTGTTGGCAGTAGATACTTAGCCTGGTACCAGCAGAAGCCTGGC10070.114076237364669No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10070.114076237364669No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9960.11283012156426049No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9960.11283012156426049No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9880.11192385552759977No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9830.1113574392546868No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9760.11056445647260867No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9640.10920505741761757No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9500.1076190918534613No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9490.10750580859887873No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9400.1064862593076354No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9390.1063729760530528No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9360.10603312628930506No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC9230.10456044397973137No Hit
GTTCTACTTTGACAACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATCCCCGACCAGCCCCAAGGTCTT9120.10331432817932286No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9090.1029744784155751No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9020.10218149563349696No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8960.10150179610600141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.002105411470.97311470
GTATACG200.00825806450.26191
CTATATC200.00826731650.2476654
AGCGAAT306.588313E-445.59967438
GGTATCA6000.042.4433821
TACTTAT350.001510901638.52178630
GTATCAA13850.035.080511
TAGATTG450.003165161733.1322264
GGTACGT500.003481464232.49387768
GTGGTGA2850.032.373770
AAAACCG1102.75756E-432.2605170
CTATAGG450.003925637331.70724353
TGGTACG500.004479201530.86367267
CATTAGT450.00458715830.71419142
TGATAGA603.7238453E-430.65376761
GGGTAGA553.8638277E-430.4617561
TATACTA450.005016606830.15735234
TATCAAC16150.029.662842
TGTTAGA10050.029.66040870
TCGTATA2000.029.42761661
Produced by FastQC (version 0.11.5)