FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360938
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53020.38958424263265484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45960.3377082570991478No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39120.2874488036927472No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38570.28340747337498107No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32550.23917327607870456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31800.23366237109993254No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG31280.22984147698131727No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGTTCTATTTGGGTT30900.22704928512540615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30420.22352230593899206No Hit
GTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATAAA29630.2177174860280189No Hit
CTACAAACTCCTCCGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACG29600.21749704982886803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28920.21250049598144807No Hit
CTTTATTACTGTGTGAGAGTCTCGAAAGATCCTATAGACTACGGTGACTA27490.20199303715525613No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26310.19332254665532156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26170.19229384439261743No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC26130.19199992946041627No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC25950.190677312265511No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25360.1863420670155437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25200.185166407286739No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24700.18149247063422436No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24640.1810515982359226No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24620.180904640769822No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24510.18009637470626877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24050.17671635298595528No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23700.17414459732919502No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22770.16731107515551774No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22480.16518019189705924No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21380.15709753126152698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21180.15562795660052112No Hit
GTCCCTGACAGGTTCAGTGTCAGTGGTTCAGGCACAGATTTTACACTGAA21000.15430533940561583No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20840.15312967967681115No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT20360.14960270049039706No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20040.14725138103278768No Hit
GTGTAAAATCTGTGCCTGAACCACTGACACTGAACCTGTCAGGGACCCCG19890.1461492000370333No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19730.1449735403082286No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGAACAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA19310.14188743352011626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19300.141813954787066No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACTCTCC19290.14174047605401568No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19270.1415935185879151No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC19130.140564816325211No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18780.1379930606684507No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATCTCC18420.13534782627864017No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAACT18380.135053911346439No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18240.13402520908373491No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGTTCTATTTGGGTTCTAATCGGGCC18220.1338782516176343No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18210.13380477288458403No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGA18000.13226171949052787No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17610.12939604890156642No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17610.12939604890156642No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17470.12836734663886232No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17010.12498732491854883No Hit
GTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAACCACTGACA17010.12498732491854883No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16770.12322383532534179No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACT16670.12248904799483885No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC16530.12146034573213475No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16440.12079903713468211No Hit
CAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAACCACTGACA16370.12028468600333005No Hit
GTTCAGTGTCAGTGGTTCAGGCACAGATTTTACACTGAAAATCAGCAGAG16230.11925598374062595No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16150.11866815387622359No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG15900.11683118554996627No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15890.11675770681691597No Hit
GTGTCAGTGGTTCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTATTA15840.11639031315166452No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15660.11506769595675923No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15650.11499421722370894No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCCGACGTTCGGCCAGGGGA15610.11470030229150778No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15390.11308377016440133No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15230.11190811043559662No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15160.11139375930424456No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15050.11058549324069135No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14970.109997663376289No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14920.10963026971103752No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTATCTCTACCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14860.10918939731273578No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14860.10918939731273578No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14540.10683807785512639No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14490.10647068418987492No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14460.10625024799072404No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14380.10566241812632171No Hit
GTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAAC14360.10551546066022112No Hit
TTCATATACTACGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTTTATCTG14250.10470719459666789No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14180.10419284346531585No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13930.10235587513905851No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13920.10228239640600821No Hit
GAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAACTCCTCCGACGTTCGGCCAGGGGA13810.10147413034245498No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGTTCTATTTGGGTTCTAAT13800.10140065160940469No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13750.10103325794415322No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTC13750.10103325794415322No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGTT13700.10066586427890177No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13680.10051890681280118No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG13640.1002249918806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)