FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294895
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC68930.5323211534525966No Hit
GCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAG61910.47810826360438496No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56180.43385757146332327No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50300.3884484842400349No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTA45980.35508670587190466No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG44780.3458195452140907No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39180.3025727954776256No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGT38930.3006421370072477No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38480.29716695176056745No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTT37350.2884403754744593No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT34920.26967437514238607No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34040.2628784573266558No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33370.257704292626043No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33310.25724093459315234No Hit
GTAATAATCAGCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG32240.2489777163399349No Hit
TCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATAT32110.24797377393533837No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31970.24689260519192677No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31290.24164121415249884No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30830.2380888025670035No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG30110.23252850617231513No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29870.23067507404075233No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29800.23013448966904654No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29650.22897609458681975No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29450.22743156781051746No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA29420.22719988879407207No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29250.22588704103421514No Hit
GACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAGCAGTAATAAT29110.22480587229080348No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28660.22133068704412326No Hit
GGATAGTTGCCTGCATATGAGCAGCAGTAATAATCAGCATCATCCTCAGC28560.2205584236559721No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27380.21144571567578838No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC27320.2109823576428977No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG27260.210518999610007No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26990.20843388846199887No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26180.2021785550179744No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGA24390.18835504037006862No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24080.18596102386680002No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC23550.18186802790959886No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAG23310.18001459577803605No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGC22470.1735275833175663No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22070.17043852976496165No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT21600.16680889184065117No Hit
ACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGC21550.1664227601465756No Hit
GGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCG21540.1663455338077605No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21400.16526436506434883No Hit
CAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA21030.16240699052818955No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGG20710.15993574768610583No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAG20650.1594723896532151No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20200.1559972044065349No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC20180.15584275172890466No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19950.15406654593615698No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT19780.1527536981763No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA19350.14943296560725No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19310.14912406025198954No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19180.148120117847393No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTC18960.1464211383934605No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18890.14588055402175465No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18750.14479938527834302No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18630.14387266921256164No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18500.1428687268079651No Hit
GCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCC18400.14209646341981397No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGG18050.1393935415612849No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18040.13931631522246976No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17900.13823514647905816No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17620.1360728089922349No Hit
GTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCA17540.13545499828171395No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT17350.13398769784422676No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17240.1331382081172605No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17070.1318253603574035No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17050.13167090767977327No Hit
GACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCT16770.12950857019295No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16530.1276551380613872No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTC16430.12688287467323606No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16240.12541557423574884No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT16180.12495221620285814No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16090.12425717915352211No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16070.12410272647589186No Hit
TCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC16030.1237938211206314No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15650.12085922024565698No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCC15570.12024140953513604No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC15430.11916024079172442No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15400.11892856177527908No Hit
CTCCCTGACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCC15150.11699790330490117No Hit
GTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT14730.1137543970746663No Hit
GCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTG14560.1124415493148093No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14430.11143760691021279No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14400.11120592789376745No Hit
ATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTT14380.11105147521613722No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14250.11004753281154071No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGA14220.10981585379509537No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13860.10703570559775118No Hit
CAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCG13800.10657234756486046No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13770.10634066854841512No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCC13530.10448723641685233No Hit
GGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA13460.10394665204514651No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC13400.10348329401225582No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13150.10155263554187792No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13020.1005486931372814No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12950.10000810876557559No Hit
GAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATC12950.10000810876557559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)