FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294895
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62470.48243293857803143No Hit
GCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAG60560.46768270786434424No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51180.3952444020557651No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49190.379876360631557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTA40810.31516068870448954No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG39530.30527571733615466No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT36370.2808721942705779No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGT35950.2776286880403431No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35640.2752346715370745No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35120.27121890191868836No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34690.2678981693496384No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34660.26766649033319306No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTT34650.26758926399437793No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31790.24550253109325465No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG29820.23028894234667674No Hit
TCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATAT29490.22774047316577792No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29290.2261959463894756No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29250.22588704103421514No Hit
GACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAGCAGTAATAAT28730.2218712714158291No Hit
GTAATAATCAGCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG28690.22156236606056864No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28570.22063564999478721No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27640.21345360048498138No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC27640.21345360048498138No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27590.21306746879090582No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27270.2105962259488221No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27020.2086655674784442No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26300.20310527108375584No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26020.20094293359693258No Hit
GGATAGTTGCCTGCATATGAGCAGCAGTAATAATCAGCATCATCCTCAGC25980.20063402824167212No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA25930.2002478965475965No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG25450.19654103228447095No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25350.19576876889631978No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23980.18518876047864885No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGA23650.18264029129775003No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23310.18001459577803605No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23010.17769780561358256No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC22610.17460875206097792No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAG22400.17298699894586048No Hit
GGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCG22290.1721375092188942No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22090.17059298244259188No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21560.16649998648539072No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20930.1616347271400384No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGC20150.1556110727124593No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19650.1517497557717035No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC19190.14819734418620817No Hit
CAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA19040.1470389491039814No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAG18780.14503106429478838No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18710.14449047992308256No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT18600.14364099019611629No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA18240.1408608419987721No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18120.1399341259329907No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18050.1393935415612849No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17960.13869850451194884No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGG17940.13854405183431862No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17930.1384668254955035No Hit
ACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGC17880.1380806938014279No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTC17520.13530054560408372No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17500.1351460929264535No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17470.13491441391000816No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17260.13329266079489072No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT17210.13290652910081513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17130.13228871839029419No Hit
GTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCA16410.12672842199560583No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16230.12533834789693374No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGG15920.12294433139366512No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT15810.12209484166669883No Hit
GACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCT15780.12186316265025349No Hit
GCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCC15740.12155425729499303No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15300.1181562983871279No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15250.11777016669305233No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15220.11753848767660699No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15200.11738403499897675No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15160.11707512964371629No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15140.11692067696608606No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT15110.11668899794964072No Hit
TCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC14600.11275045467006978No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTC14400.11120592789376745No Hit
CTCCCTGACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCC14370.1109742488773221No Hit
GCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTG14170.10942972210101977No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCC13810.10664957390367558No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13790.10649512122604535No Hit
GTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT13720.10595453685433955No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13690.10572285783789419No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13600.10502782078855816No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC13410.10356052035107094No Hit
GGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA13170.10170708821950815No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC13160.10162986188069303No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC13140.1014754092030628No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13060.10085759849254186No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13060.10085759849254186No Hit
ATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTT13020.1005486931372814No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC12950.10000810876557559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)