FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771440
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32250.41804936223167066No Hit
GCGTTACACCTGGCCGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25510.33068028621798196No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24320.31525458882090635No Hit
GTAATAAACGGCCGTATCGTCGTCTGTCAGGCTGTTCATTTGAAGATACA23050.29879186975007777No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA23050.29879186975007777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20260.26262573887794255No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT19520.2530332883957275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19480.252514777558851No Hit
CTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19290.25005185108368766No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAT19240.24940371253759203No Hit
GTGTAACGCTGGTGACAGTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC19060.24707041377164782No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18700.2424038162397594No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17910.23216322721144872No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17710.22957067302706627No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17430.22594109716893082No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17050.22101524421860416No Hit
GCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCACCAGCGTTACACCTGGCCGTACACTTTTGGCCTGGGGA16740.21699678523281135No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16100.20870061184278754No Hit
GGCCAAAAGTGTACGGCCAGGTGTAACGCTGGTGACAGTAATAAACCGCA15780.2045525251477756No Hit
GTTTATTACTGTGCGAGAGGTGTCATAGGCGTGCCTGGAACTAGTGGTGC15760.20429326972933737No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACATCTACTTAGCCTGGTACCAACAGAGACCTGGCCTGCCTCCCAGGCT15540.20144146012651665No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15400.19962667219744892No Hit
GTACCAACAGAGACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATCCAACAGGGCCACAGGCATCCCCGC15160.19651560717619No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15040.1949600746655605No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCGGGGATGCCTGTGGCCCTGTT14980.19418230841024578No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14790.19171938193508242No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14490.18783055065850876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14260.18484911334646895No Hit
GTACCCAGACTCCGTGGAGGCATACTACGCAGACTCCGTGACGGGCCGGT14170.18368246396349683No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGT14060.1822565591620865No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGCACCTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG13840.17940474955926577No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGTG13450.17434926889972002No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13140.1703308099139272No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12950.16786788343876388No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12920.1674790003111065No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12780.16566421238203877No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12520.1622938919423416No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12430.1611272425593695No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11900.154256973970756No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11770.1525718137509074No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11740.15218293062325003No Hit
GTATTAACATCTACTTAGCCTGGTACCAACAGAGACCTGGCCTGCCTCCC11710.15179404749559267No Hit
TCCATGATGCATCCAACAGGGCCACAGGCATCCCCGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11640.1508866535310588No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11320.14673856683604689No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11190.14505340661619828No Hit
GTTTATTACTGTCACCAGCGTTACACCTGGCCGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAA11120.14414601265166443No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11120.14414601265166443No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11100.1438867572332262No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCACCAGCGTTACACCTGG11090.14375712952400704No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11070.1434978741055688No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11000.14259048014103495No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10660.13818313802758478No Hit
GAGTATTAACATCTACTTAGCCTGGTACCAACAGAGACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATC10650.13805351031836566No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10580.13714611635383178No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10560.13688686093539354No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10550.13675723322617442No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10280.1332572850772581No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10210.13234989111272427No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10140.1314424971481904No Hit
GTCTTTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACATCTACTTAGCCTGGTA9940.12884994296380795No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9730.12612776107020637No Hit
GTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9610.12457222855957689No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9520.12340557917660479No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9450.12249818521207093No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9360.12133153582909882No Hit
CTGTTGGTACCAGGCTAAGTAGATGTTAATACTCTGACTGGCCCTGCAGGAGAGGGTGACTCTTTCTCCTGGAGA9190.11912786477237373No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC9180.11899823706315463No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9170.1188686093539355No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGTGACCCATGTCCCCTGG9120.1182204708078399No Hit
GTCTGGGTACCGTGTCTCACTCGCACCACCGCTAATAGTGGAGACCCAGT9120.1182204708078399No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9070.11757233226174428No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8950.1160167997511148No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8920.11562791662345744No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8900.1153686612050192No Hit
GGTGTAACGCTGGTGACAGTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT8890.11523903349580007No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8850.11472052265892357No Hit
CTATTAGCGGTGGTGCGAGTGAGACACGGTACCCAGACTCCGTGGAGGCA8840.11459089494970445No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8800.11407238411282797No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8770.11368350098517059No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8730.1131649901482941No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8730.1131649901482941No Hit
ACAGAGACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATCCAACAGGGCCACAGGCATCCCCGCCAGGTT8670.11238722389297937No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCGGGGATGCCT8450.10953541429015866No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8430.10927615887172042No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGGAATTGTGTTGACACAGTC8270.10720211552421445No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8270.10720211552421445No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8250.10694286010577621No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT8240.10681323239655707No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8190.10616509385046147No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG8150.10564658301358497No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8040.10422067821217465No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGTGACCCATGTCCCCTGG7960.10318365653842167No Hit
GACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATCCAACAGGGCC7900.10240589028310693No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7900.10240589028310693No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7890.1022762625738878No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7870.10201700715544955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATCG250.001474820777.6347470
ATGCCGT353.150417E-759.5036744
TCGTATG452.9636794E-853.81011640
CACACCG200.00660041253.19171562
GTGGTAT1100.049.781061
CTAATCT352.3183873E-549.11816834
CGAGACT507.3860065E-847.9204823
ACGAGAC507.389826E-847.91737422
CGTATGC451.8135379E-646.17135641
GGTATCA5350.046.059111
CTCGCTA306.560265E-445.6385523
AATCTCG503.891784E-641.32114836
TGGTATC1350.040.5623442
GTATCAA14650.040.1816371
ATCTCCG603.0961928E-739.93114510
CACGAGA603.0961928E-739.93114521
CCCACGA603.0977753E-739.92855519
CTTATAC702.3477696E-839.1136861
TTAACGG451.0195771E-438.2154635
ACGGGGT1001.0913936E-1137.64936416
Produced by FastQC (version 0.11.5)