FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771440
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31630.41001244426008504No Hit
GCGTTACACCTGGCCGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24150.31305091776418126No Hit
GTAATAAACGGCCGTATCGTCGTCTGTCAGGCTGTTCATTTGAAGATACA22890.29671782640257177No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA21040.2727367001970341No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20570.26664419786373533No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20270.2627553665871617No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT20140.2610702063673131No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAT19320.250440734211345No Hit
GTGTAACGCTGGTGACAGTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC18980.24603339209789485No Hit
CTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18920.2452556258425801No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18690.24227418853054028No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18010.23345950430363996No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17750.23008918386394275No Hit
GCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCACCAGCGTTACACCTGGCCGTACACTTTTGGCCTGGGGA16100.20870061184278754No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15990.20727470704137718No Hit
GTTTATTACTGTGCGAGAGGTGTCATAGGCGTGCCTGGAACTAGTGGTGC15680.20325624805558437No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACATCTACTTAGCCTGGTACCAACAGAGACCTGGCCTGCCTCCCAGGCT15450.20027481074354456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14980.19418230841024578No Hit
GGCCAAAAGTGTACGGCCAGGTGTAACGCTGGTGACAGTAATAAACCGCA14860.1926267758996163No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCGGGGATGCCTGTGGCCCTGTT14770.19146012651664418No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14660.19003422171523385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14560.18873794462304264No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGCACCTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG14190.1839417193819351No Hit
GTACCAACAGAGACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATCCAACAGGGCCACAGGCATCCCCGC14080.18251581458052474No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14080.18251581458052474No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGT13920.18044177123301877No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGTG13590.17616405682878772No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13580.1760344291195686No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13270.17201597013377581No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13260.1718863424245567No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13160.17059006533236545No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13040.16903453282173597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12960.167997511147983No Hit
GTACCCAGACTCCGTGGAGGCATACTACGCAGACTCCGTGACGGGCCGGT12800.16592346780047704No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12790.16579384009125792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12720.16488644612672404No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12370.16034947630405477No Hit
TCCATGATGCATCCAACAGGGCCACAGGCATCCCCGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12000.1555532510629472No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11730.1520533029140309No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11630.15075702582183967No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11420.1480348439282381No Hit
GTATTAACATCTACTTAGCCTGGTACCAACAGAGACCTGGCCTGCCTCCC11310.14660893912682776No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11290.14634968370838952No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCACCAGCGTTACACCTGG11190.14505340661619828No Hit
GTTTATTACTGTCACCAGCGTTACACCTGGCCGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAA11180.14492377890697916No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11160.14466452348854092No Hit
GTCTTTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACATCTACTTAGCCTGGTA11150.1445348957793218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11060.14336824639634968No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10730.13909053199211863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10690.13857202115524214No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10690.13857202115524214No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10630.13779425489992742No Hit
GAGTATTAACATCTACTTAGCCTGGTACCAACAGAGACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATC10480.13584983926164057No Hit
GGTGTAACGCTGGTGACAGTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT10320.1337757959141346No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10250.13286840194960076No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10220.1324795188219434No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10180.1319610079850669No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9950.12897957067302707No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9910.12846105983615058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9820.12729441045317846No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9620.12470185626879601No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9570.12405371772270041No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9570.12405371772270041No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9330.12094265270144146No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9210.11938712019081198No Hit
GTCTGGGTACCGTGTCTCACTCGCACCACCGCTAATAGTGGAGACCCAGT9190.11912786477237373No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9180.11899823706315463No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9080.1177019599709634No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9080.1177019599709634No Hit
GTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9010.11679456600642954No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8920.11562791662345744No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCGGGGATGCCT8840.11459089494970445No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8800.11407238411282797No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGTGACCCATGTCCCCTGG8730.1131649901482941No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC8710.11290573472985585No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8640.111998340765322No Hit
CTGTTGGTACCAGGCTAAGTAGATGTTAATACTCTGACTGGCCCTGCAGGAGAGGGTGACTCTTTCTCCTGGAGA8620.11173908534688375No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8570.11109094680078814No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8420.1091465311625013No Hit
CTATTAGCGGTGGTGCGAGTGAGACACGGTACCCAGACTCCGTGGAGGCA8390.10875764803484392No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGTGACCCATGTCCCCTGG8370.10849839261640568No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8310.10772062636109093No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGGAATTGTGTTGACACAGTC8270.10720211552421445No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8220.10655397697811883No Hit
ACAGAGACCTGGCCTGCCTCCCAGGCTCCTCATCCATGATGCATCCAACAGGGCCACAGGCATCCCCGCCAGGTT8180.10603546614124235No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8050.10435030592139377No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7820.10136886860935394No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7770.10072073006325832No Hit
GGGCTGGACTGGGTCTCCACTATTAGCGGTGGTGCGAGTGAGACACGGTA7730.10020221922638184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA250.001310108679.9852770
TACGTAT250.001310108679.9852770
GGTACGT200.00483745657.53711768
GTGGTAT1500.052.34421
ACCGATC200.00724067551.96095342
CGGCACT200.007644384651.25280816
CCTAATA200.00767581551.1996462
TGGCCTA200.007677782351.1963235
AGTATAC200.007679750751.1930053
AGGTATC352.420463E-548.7615662
TGGTATC1700.046.1800732
GGTATCA5750.044.5272031
GTAGTTT754.186446E-544.43625670
GTATCAA15000.040.0546951
AATGGTA2650.037.72889770
GTACTGC853.2814569E-936.154996
CCAACCG501.806356E-434.6748643
GTCGCCC601.3534702E-534.45737
ATGGCAA1609.586984E-833.32719470
ATACGTA959.667929E-932.36599311
Produced by FastQC (version 0.11.5)