FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626544
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACAGCCGTGTCCTCGGGTCTCAGGCTGCTCATTTCCAGATACA79720.49011892700105253No Hit
GTTTACTACTGTGCGAGAGATTATTATGATTTTTGGAGTGATTATCGTCC59450.3654988736855566No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45730.28114825052381No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG43720.26879076127052204No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC43020.26448715804798395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37600.23116497309633183No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG36160.22231184646711064No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTCTCA35970.22114372559242174No Hit
GTATATTATTGTCTCGTCTACGATGGCTCATTTCGGTGGACGTTTGGCCA34130.20983139712175014No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG31720.19501470602701187No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31650.19458434570475805No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31280.1923095840014165No Hit
ATAATATACTGCAAAGTCTTCAGGCTCCAGTCGGCCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30950.19028074248221996No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30320.1864074995819357No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30090.18499345852310173No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG29810.1832720172340865No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG29080.17878397387343964No Hit
CTCCTGTGCAGCCTCTGGATTCATTTTCAGTGTTTGCAGCATGAATTGGGTCCGCCAGGCTCCAGGGAAAGGG28640.17607885184784425No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28170.1731892896841401No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC27590.16962344701403714No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27360.16820940595520317No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGTCCCTGTA26710.1642132029628464No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA26580.163413962364375No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26550.16322952222626624No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26550.16322952222626624No Hit
GTCTCCAGGCACCCTGTCCCTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGGCCAGTCAGCATGTTAGCAG26540.16316804218022998No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC26340.16193844125950482No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC26030.16003255983238082No Hit
CTCCTGTAGGGCCAGTCAGCATGTTAGCAGCTCCTACTTACTTTGGATCC25820.15874147886561937No Hit
ATATTATTGTCTCGTCTACGATGGCTCATTTCGGTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAACGAA25690.157942238267148No Hit
GTCTGGGACAGACTTCACTCTCACCATCGGCCGACTGGAGCCTGAAGACTTTGCAGTATATTATTGTCTCGTCTA25630.15757335799093045No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25630.15757335799093045No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24500.15062611278883326No Hit
ATCGTAGACGAGACAATAATATACTGCAAAGTCTTCAGGCTCCAGTCGGC24370.1498268721903619No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23960.1473061903028753No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG23820.1464454696583677No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG23300.14324850726448224No Hit
GAGTAATAGGTGTGAACACCACTAGCCCCAATGGAAGCGACCCACTCCAGCCCTTTCCCTGGAGCCTGGCGGACC23010.14146558592943076No Hit
GTCTACGATGGCTCATTTCGGTGGACGTTTGGCCAAGGGACCAAGGTGGA22770.13999006482456053No Hit
TCCTACAGGGACACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCGGC22680.13943674441023424No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT22650.13925230427212543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22580.13882194394987163No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAATTCATGCTGCAAACAC22290.13703902261482012No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCAAACGTC22160.13623978201634876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22020.13537906137184114No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21370.13138285837948435No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21110.12978437718254165No Hit
TCTCTGGGACATCCTACAGGGACACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT21090.12966141709046913No Hit
CTCCAGGCACCCTGTCCCTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGGCCAGTCAGCATGTTAGCAGCT20760.12763257557127258No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC20570.12646445469658368No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20490.12597261432829363No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20450.12572669414414858No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC19830.12191493128990054No Hit
CCTATTACTCAGACTCAGTGAAGGGCCGATTCACCGTCTCCCGAGACGAC19740.12136161087557423No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19630.12068533036917538No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19580.12037793013899409No Hit
CCACTAGCCCCAATGGAAGCGACCCACTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAATTCATGCTGCAAACA19470.11970164963259525No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19310.1187179688960151No Hit
GTCTGGGGGAGGCCTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTG19240.1182876085737613No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19150.11773428815943497No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG18890.11613580696249225No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCGGCCGAC18510.11379956521311443No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT18490.11367660512104191No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18490.11367660512104191No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18420.1132462447987881No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCAGGGTTCCCTGG18180.11177072369391791No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG18080.11115592323355532No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATTTTCAGTGTTTGCAGC17790.10937300189850381No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17740.10906560166832253No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17630.1083893211619237No Hit
GCCTGAGACCCGAGGACACGGCTGTTTACTACTGTGCGAGAGATTATTATGATTTTTGGAGTGATTATCGTCCGC17600.10820488102381491No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC17450.10728268033327103No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17410.107036760149126No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTCGCTATTTTAGAAGGT17170.1055612390442558No Hit
CCTGTAGGGCCAGTCAGCATGTTAGCAGCTCCTACTTACTTTGGATCCAGCAGAGACCTGGCCAGCCTCCCAGG17100.10513087872200198No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17090.10506939867596574No Hit
GGCTAGTGGTGTTCACACCTATTACTCAGACTCAGTGAAGGGCCGATTCACCGTCTCCCGAGACGACGCCAAGAA17050.10482347849182069No Hit
CCCTGTCCCTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGGCCAGTCAGCATGTTAGCAGCTCCTACTTA16950.10420867803145811No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16870.10371683766316805No Hit
GACTTCACTCTCACCATCGGCCGACTGGAGCCTGAAGACTTTGCAGTATA16820.10340943743298674No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16810.1033479573869505No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16780.10316351724884171No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCAGGGTTCCCTGG16730.10285611701866043No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT16630.10224131655829784No Hit
CAATAATATACTGCAAAGTCTTCAGGCTCCAGTCGGCCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16440.10107319568360892No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTCT16370.10064283536135511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC150.002222339270.0216151
CGATCAT150.002460063768.2499110
CGTATGC609.640644E-1151.8247541
TCGCTAA606.008122E-945.648731
GGTATCA10050.044.8180851
TCGGGAG21550.042.4277770
TCGTATG758.6947693E-1041.4121140
CGAAATT655.0454946E-737.5240448
CGCTAAT754.269532E-836.5516632
GTATCAA23700.035.4186671
CCCGAAA651.516223E-533.8934667
GTGGTAT2650.033.479031
ATGCCGT958.400093E-932.84079744
AATCTCG851.2503187E-732.34633336
ACACTCG851.3149656E-732.16111427
AATGGTA6400.031.63378370
TATCAAC26700.031.3112372
GCCGCTA550.0042115131.25643569
TCGGGTC18850.031.14172220
CTCGGGA22100.030.92053469
Produced by FastQC (version 0.11.5)