FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626544
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACAGCCGTGTCCTCGGGTCTCAGGCTGCTCATTTCCAGATACA72890.448128055558288No Hit
GTTTACTACTGTGCGAGAGATTATTATGATTTTTGGAGTGATTATCGTCC53010.32590572403820617No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46450.2855748138384206No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG38780.23841961852861035No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC36940.22710729005793878No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC35830.2202830049479141No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTCTCA33620.206695914773901No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG33180.20399079274830562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32540.2000560698019851No Hit
GTATATTATTGTCTCGTCTACGATGGCTCATTTCGGTGGACGTTTGGCCA31540.19390806519835924No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31380.1929243844617791No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG30090.18499345852310173No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG29660.18234981654354263No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29470.18118169566885373No Hit
ATAATATACTGCAAAGTCTTCAGGCTCCAGTCGGCCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29170.17933729428776596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29000.17829213350514958No Hit
CTCCTGTGCAGCCTCTGGATTCATTTTCAGTGTTTGCAGCATGAATTGGGTCCGCCAGGCTCCAGGGAAAGGG28370.1744188906048653No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27650.16999232729025468No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27060.1663650045741154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27050.16630352452807917No Hit
GTCTGGGACAGACTTCACTCTCACCATCGGCCGACTGGAGCCTGAAGACTTTGCAGTATATTATTGTCTCGTCTA26680.1640287628247376No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA26670.16396728277870135No Hit
CTCCTGTAGGGCCAGTCAGCATGTTAGCAGCTCCTACTTACTTTGGATCC25530.1569585575305679No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25510.15683559743849537No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC25130.15449935568911755No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC24810.15253199421595726No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24780.1523475540778485No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGTCCCTGTA24580.15111795315712334No Hit
GTCTCCAGGCACCCTGTCCCTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGGCCAGTCAGCATGTTAGCAG24320.14951947196018062No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC23530.14466254832331618No Hit
ATATTATTGTCTCGTCTACGATGGCTCATTTCGGTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAACGAA23410.14392478777088108No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG22740.13980562468645177No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAATTCATGCTGCAAACAC22400.137715303121219No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC22370.1375308629831102No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22160.13623978201634876No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22150.1361783019703125No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22060.1356249815559862No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCAAACGTC21910.13470278086544232No Hit
ATCGTAGACGAGACAATAATATACTGCAAAGTCTTCAGGCTCCAGTCGGC21780.13390354026697096No Hit
GTCTACGATGGCTCATTTCGGTGGACGTTTGGCCAAGGGACCAAGGTGGA21460.13193617879381067No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21170.1301532574587592No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20900.1284932962157802No Hit
TCCTACAGGGACACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCGGC20750.12757109552523632No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20690.12720221524901879No Hit
GAGTAATAGGTGTGAACACCACTAGCCCCAATGGAAGCGACCCACTCCAGCCCTTTCCCTGGAGCCTGGCGGACC20460.12578817419018484No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20240.12443561317738713No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20030.12314453221062571No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20000.12296009207251694No Hit
CTCCAGGCACCCTGTCCCTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGGCCAGTCAGCATGTTAGCAGCT19990.12289861202648068No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19660.12086977050728416No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19510.11994756981674028No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19080.11730392783718116No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC19060.11718096774510865No Hit
GTCTGGGGGAGGCCTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTG18930.1163817271466373No Hit
GCCTGAGACCCGAGGACACGGCTGTTTACTACTGTGCGAGAGATTATTATGATTTTTGGAGTGATTATCGTCCGC18630.11453732576554954No Hit
CCTATTACTCAGACTCAGTGAAGGGCCGATTCACCGTCTCCCGAGACGAC18490.11367660512104191No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCAGGGTTCCCTGG18440.11336920489086062No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18370.11293884456860681No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18320.11263144433842553No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC18110.1113403633716641No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18090.11121740327959158No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAAGGCGGACGATAATCACTCCAAAAA18020.11078704295733777No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17910.11011076245093894No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCGGCCGAC17910.11011076245093894No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17860.10980336222075762No Hit
TCTCTGGGACATCCTACAGGGACACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17670.10863524134606872No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT17520.10771304065552484No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG17520.10771304065552484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17510.10765156060948858No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG17480.10746712047137981No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCAGGGTTCCCTGG17270.10617603950461839No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATTTTCAGTGTTTGCAGC17160.10549975899821955No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16820.10340943743298674No Hit
CCTGTAGGGCCAGTCAGCATGTTAGCAGCTCCTACTTACTTTGGATCCAGCAGAGACCTGGCCAGCCTCCCAGG16790.10322499729487798No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16790.10322499729487798No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA16780.10316351724884171No Hit
GGCTAGTGGTGTTCACACCTATTACTCAGACTCAGTGAAGGGCCGATTCACCGTCTCCCGAGACGACGCCAAGAA16650.10236427665037036No Hit
CCACTAGCCCCAATGGAAGCGACCCACTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAATTCATGCTGCAAACA16420.1009502355915364No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16390.10076579545342763No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16280.10008951494702878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)