FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435635
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57230.3986389298115468No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49400.3440986044502955No Hit
GAGTTACAGTACCCCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT46650.32494331776530944No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43700.30439491932141527No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGATTTCA39790.2771595844347623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38710.2696367809366587No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38150.26573606801171606No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36400.25354634012127036No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34950.2434462798691868No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC32840.22874895081270658No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGACAAGTCAGAGCATTAGCATCTATTTAAATTGGTATCAACAGAAA32640.2273558390537985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32430.225893071706945No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31530.21962406879185867No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATT30810.21460886645978958No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30750.21419093293211713No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30230.21056884235895615No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28900.2013046491622174No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGACAAGTCAGAGCATTAGCAT28860.2010260268104358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28720.20005084857920014No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28690.19984188181536394No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28370.197612903001111No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27840.1939211568400046No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGTGAGGGGTA26960.18779146510080905No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26010.18117418424599568No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25820.179850728075033No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24710.17211895781309314No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA24670.17184033546131156No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24130.1680789337122597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23910.16654651077746085No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT23630.16459615431498953No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23270.16208855314895498No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC23170.16139199726950096No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22850.15916301845524802No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAAT22850.15916301845524802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22540.1570036952289405No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22080.15379953818345193No Hit
AGTCAGAGCATTAGCATCTATTTAAATTGGTATCAACAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG21880.15240642642454383No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21830.1520581484848168No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCA21700.15115262584152658No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21340.14864502467549204No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21000.1462767346853483No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20710.14425672263493158No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20610.14356016675547756No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT19980.1391718647149171No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGTTT19860.13833599765957225No Hit
GTTACAGTACCCCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA19780.137778752956009No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19100.13304217297572155No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA18750.13060422739763242No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGACAAGTCAGA18630.12976836034228756No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18620.12969870475434214No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18390.12809662623159784No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18310.12753938152803462No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17840.12426556889460064No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17840.12426556889460064No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17670.12308142389952878No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17070.11890208862280455No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAAACT16970.1182055327433505No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16490.11486206452197112No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16480.1147924089340257No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16450.11458344217018951No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAATAGATGCTA16440.11451378658224409No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16370.11402619746662626No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16290.11346895276306303No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16140.11242411894388196No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16080.11200618541620955No Hit
GTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCACTTTGCAAACT15910.11082204042113769No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT15810.11012548454168364No Hit
GTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGATTTCACT15750.10970755101401124No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15680.10921996189839339No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15670.10915030631044799No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCACACTTT15660.10908065072250259No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCACACTTTCGGCGGAGGGA15450.1076178833756491No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15420.10740891661181289No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15190.10580683808906861No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15160.10559787132523239No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15120.10531924897345078No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14880.10364751486276107No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14880.10364751486276107No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT14840.10336889251097946No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14800.10309027015919785No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA14680.102254403103853No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG14610.10176681398823519No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14560.10141853604850816No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT14540.10127922487261733No Hit
ACAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGTTT14500.10100060252083573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT307.247443E-444.7275774
CGTATGC4550.040.8979841
GTGGTAT4200.040.7340471
ATGCCGT4700.040.66495544
TCGTATG4550.039.2988740
CCGTCTT5300.036.9530447
GACTAGG5100.036.92321826
ATCTCCG5150.036.4743210
CGAGACT5100.036.2044623
ACGAGAC5100.036.19436622
TAGGCAT5250.035.9725229
CTAGGCA5250.035.93728328
CACGAGA5100.035.5288621
GTATGCC5300.035.22143642
ACTAGGC5300.034.92225327
ATCTCGT3950.034.56752837
CCCACGA5350.034.48861719
TCTCCGA5450.034.46655711
GTCGTAT701.0175227E-634.3068939
CTTATAC5650.033.2488171
Produced by FastQC (version 0.11.5)