FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170871
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGGCTGTCAGGCTGTTCATTTGAAGAAACA48470.41396533008333114No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC36310.3101110199159429No Hit
GTATAATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT36230.30942776787536797No Hit
GTGTATTACTGTGCAAGAGAACCTTCTGATTGCAGTGATAATAACTGCTA35980.3072926052485714No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28680.2449458565461097No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24660.21061244150721983No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20230.1727773597603835No Hit
ATCCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAAATGCCAAG19190.16389508323290952No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18770.16030801001989117No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18710.15979557098945998No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17600.15031544892648294No Hit
GAGTATTAGCAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGT16740.1429704894903025No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16550.14134776589393708No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16540.1412623593888652No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16450.1404937008432184No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15960.13630878209469702No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15930.13605256257948142No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15880.1356255300541221No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15670.13383199344761293No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15610.13331955441718174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15300.1306719527599539No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15120.12913463566866032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15040.12845138362808542No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14880.12708487954693556No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14640.1250351234252108No Hit
CTATCCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAAATGCCAAGAACTCCTTGTTTCTTCAAATGAA14260.12178967623247992No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCCCTACGACATGCACTGGGTCCGCCAAGTTACAGGAAAAGGT14220.12144805021219246No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13880.11854422903974904No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13790.11777557049410226No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13320.11376146475572457No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13310.11367605825065273No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGCAGGTGGTTGGCCTGGTATCAGCAGAAA12860.10983276552241879No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12490.10667272483475976No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12460.10641650531954416No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12290.10496459473332245No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12270.10479378172317873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12160.1038543101673882No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12060.10300024511666955No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11980.10231699307609464No Hit
CAGTAATACACAGCCGTGTCCCCGGCTGTCAGGCTGTTCATTTGAAGAAA11920.10180455404566345No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA11870.10137752152030412No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11820.1009504889949448No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11730.10018183044929801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG700.064.07110640
CGTATGC850.056.91689341
TATCTCG1000.051.50442536
TACGTAT759.351352E-750.82679470
GACCGAT1250.046.33790626
ACGAGAC1400.041.3324822
ACCGATC1450.039.95672227
ATCTCCG1450.039.8935910
AGACCGA1500.038.59676725
GTACGTA805.20753E-737.3701169
GTATCAA26250.036.815421
CCGATCA1650.035.1360328
ATCTCGT1500.034.3866737
GGCCGTG13250.034.0443670
TATACAC1950.033.1558153
TATCAAC29700.032.5388832
TTATGCC552.6339668E-432.5195160
TCAACGC30100.032.4456254
ATCAACG30000.032.326923
CCCACGA1800.032.1419919
Produced by FastQC (version 0.11.5)