FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883464
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAGCTGATTCCTGGACGTGTCCACTGA39520.44733005532766473No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36000.40748689250495773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31060.35157063558899965No Hit
GTCTCTGGTGGCTCCATCAGCAGTGATAGGTACTACTATTGGAGCTGGAT29970.3392328380103773No Hit
GTATATTACTGTGCGAGAAACAAAATCAGTGGCTGGATAATAGACTCATGGGGCCAGGGAACCCTGGTCACCGT27970.31659467731565744No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23940.2709787835157969No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23540.26645115137685293No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22420.2537737813878098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21920.24811424121412984No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG21710.24573723434118425No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21410.24234151023697628No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21280.24087002979181946No Hit
GAATATTGGTACGTGGTTGACCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATCAGGCGT21140.23928535854318908No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATAGGTACTACTATT20600.23317305515561473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20310.22989052185488032No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19440.22004292195267722No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19420.21981654034572998No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18680.21144042088868364No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTACGTGGTTGACCTGGTATCAGCAGAAA18340.20759193357058123No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18300.20713917035668686No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18100.20487535428721487No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17700.2003477221482709No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17610.1993290049170085No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA17600.1992158141135349No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17040.19287712911901334No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16830.19050012224606777No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16610.18800992456964857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16330.18484058207238777No Hit
CCCTGAGGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTATATTACTGT15440.17476660056323742No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15400.17431383734934303No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15230.17238959369029186No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15120.17114449485208225No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15050.17035215922776706No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14650.1658245270888231No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14550.1646926190540871No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC14480.1639002834297719No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14430.1633343294124039No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14030.15880669727345992No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13860.15688245361440872No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13830.15654288120398793No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13540.15326034790325357No Hit
GTACTACTATTGGAGCTGGATCCGGCAGTCCGCCGGGAAGGGACTGGAGT13410.15178886745809678No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13400.15167567665462317No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13140.14873271576430958No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC12860.14556337326704882No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12840.14533699166010158No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12530.14182807675242004No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12480.14126212273505204No Hit
GCCTCAGGGAGAGCTGATTCCTGGACGTGTCCACTGACATGGTGACTCGA12420.14058297791421043No Hit
CTCCTAATCTATCAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTT12350.13979064228989524No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAACATGATAATTATGTGACTTTCGG12330.13956426068294803No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12280.13899830666558002No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11960.13537620095442485No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11850.13413110211621526No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATGAGTCTATTATCCAGCCA11530.13050899640506008No Hit
GGAGTGGATTGGGCGTGTCTATCCCACTGGGAGCACCAACTACAACCCCT11530.13050899640506008No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11530.13050899640506008No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11500.13016942399463927No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC11380.1288111343529561No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11350.12847156194253528No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11170.12643412748001048No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11160.1263209366765369No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10950.12394392980359131No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10840.12269883096538171No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10760.12179330453759293No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10740.12156692293064572No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10700.12111415971675134No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10630.12032182409243615No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10620.12020863328896253No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10580.11975587007506815No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10510.11896353445075293No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG10340.11703929079170175No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAGCTGATTCCTGGACGTGTCCA10280.11636014597086017No Hit
GATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGTCAACC10140.11477547472222976No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10070.11398313909791458No Hit
CCAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA9860.11160613222496898No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9860.11160613222496898No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9770.1105874149937066No Hit
GGACTGGAGTGGATTGGGCGTGTCTATCCCACTGGGAGCACCAACTACAA9710.10990827017286499No Hit
ACCTTGATGGGACCCCACTTTCTAAATTAGACGCCTGATAGATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9640.1091159345485498No Hit
ACTTATTACTGCCAACAACATGATAATTATGTGACTTTCGGCCCTGGGAC9560.108210408120761No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9550.1080972173172874No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9510.107644454103393No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9380.10617297365823622No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9360.10594659205128902No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT9300.10526744723044741No Hit
CTACAACCCCTCCTTCGAGAGTCGAGTCACCATGTCAGTGGACACGTCCAGGAATCAGCTCTCCCTGAGGCTGAG9280.10504106562350023No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9170.10379596678529061No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATCAGGCGTCTAATTTAGAAAGTGGGGTCCCAT9080.10277724955402824No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9060.10255086794708104No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8930.10107938750192424No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8870.10040024268108264No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8850.10017386107413545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)