FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883464
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAGCTGATTCCTGGACGTGTCCACTGA37580.4253710394537865No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36930.41801363722800244No Hit
GTCTCTGGTGGCTCCATCAGCAGTGATAGGTACTACTATTGGAGCTGGAT29190.33040395533943656No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27200.3078789854481903No Hit
GTATATTACTGTGCGAGAAACAAAATCAGTGGCTGGATAATAGACTCATGGGGCCAGGGAACCCTGGTCACCGT27020.3058415509856655No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25170.28490125234304964No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23780.2691677306602193No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22530.25501888022601943No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21320.24132279300571385No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20380.23068285747919554No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19540.2211748299874132No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATAGGTACTACTATT19230.2176659150797316No Hit
GAATATTGGTACGTGGTTGACCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATCAGGCGT18850.2133646645477348No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18560.21008213124700045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18450.20883703240879084No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG18200.20600726232195085No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17970.2034038738420581No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17500.19808390607879892No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17440.1974047612579573No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17360.19649923483016854No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16910.19140564867385654No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16580.18767035215922775No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16500.186764825731439No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA16440.18608568091059738No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16280.1842746280550198No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTACGTGGTTGACCTGGTATCAGCAGAAA16270.18416143725154618No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15810.17895466029176063No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15690.17759637065007744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15690.17759637065007744No Hit
CCCTGAGGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTATATTACTGT14990.16967301440692545No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14780.16729600753397988No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14370.1626551845915623No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14240.1611837041464055No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13980.15824074325609191No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13780.15597692718661993No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13680.15484501915188395No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13360.15122291344072877No Hit
GTACTACTATTGGAGCTGGATCCGGCAGTCCGCCGGGAAGGGACTGGAGT13330.15088334103030798No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13290.15043057781641359No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC13240.14986462379904555No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13150.14884590656778318No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13080.148053570943468No Hit
CTCCTAATCTATCAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTT12970.14680847210525838No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC12920.1462425180878904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12790.1447710376427336No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12670.14341274800105042No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12200.13809278023779126No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12120.13718725381000246No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12070.13662129979263446No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12030.13616853657874003No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11960.13537620095442485No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11810.13367833890232086No Hit
GGAGTGGATTGGGCGTGTCTATCCCACTGGGAGCACCAACTACAACCCCT11700.13243324006411125No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11660.13198047685021688No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATGAGTCTATTATCCAGCCA11530.13050899640506008No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11510.13028261479811287No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11310.1280187987286409No Hit
GCCTCAGGGAGAGCTGATTCCTGGACGTGTCCACTGACATGGTGACTCGA11270.1275660355147465No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11150.1262077458730633No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAACATGATAATTATGTGACTTTCGG11130.1259813642661161No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10760.12179330453759293No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10750.12168011373411933No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10660.12066139650285693No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10610.12009544248548892No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAGCTGATTCCTGGACGTGTCCA10600.11998225168201535No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10580.11975587007506815No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10560.11952948846812093No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10430.11805800802296416No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10110.11443590231180896No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG10090.11420952070486177No Hit
GGACTGGAGTGGATTGGGCGTGTCTATCCCACTGGGAGCACCAACTACAA10080.11409632990138817No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9990.11307761267012578No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9920.1122852770458106No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9810.11104017820760098No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9750.11036103338675939No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9680.1095686977624442No Hit
ACTTATTACTGCCAACAACATGATAATTATGTGACTTTCGGCCCTGGGAC9580.1084367897277082No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9530.1078708357103402No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9520.10775764490686662No Hit
GGGTTCCCTGGCCCCATGAGTCTATTATCCAGCCACTGATTTTGTTTCTCGCACAGTAATATACGGCCGTGTCTG9460.10707850008602501No Hit
CCAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA9460.10707850008602501No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9460.10707850008602501No Hit
GTCCTGTCCCAGGTGCAACTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC9430.10673892767560421No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9360.10594659205128902No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9340.10572021044434182No Hit
CTACAACCCCTCCTTCGAGAGTCGAGTCACCATGTCAGTGGACACGTCCAGGAATCAGCTCTCCCTGAGGCTGAG9330.10560701964086822No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9310.10538063803392102No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9280.10504106562350023No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9250.10470149321307942No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9180.10390915758876423No Hit
ACCTTGATGGGACCCCACTTTCTAAATTAGACGCCTGATAGATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9110.10311682196444903No Hit
GATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGTCAACC9060.10255086794708104No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTC9060.10255086794708104No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9000.10187172312623943No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8950.10130576910887144No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8890.10062662428802983No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8860.10028705187760906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG200.007532414551.44507629
AATGGTA3100.045.05185370
GTATCAA11450.039.1257361
TATCAAC13600.032.6778642
TGTCCGT552.700066E-432.38134864
ATCAACG14000.031.9883943
CAACGCA14350.031.2099555
TCAACGC14250.031.1855264
ACGGGTA553.4118377E-431.1152316
GCCGTTG450.004398866630.97850449
AACGCAG14600.030.6824876
GTGGTAT1351.0913936E-1130.397941
TAAGTCA1104.0379082E-430.22960370
ACGCAGA15500.028.9107447
CGGGTCA605.720922E-428.486784
AGAGTAC15650.028.42955811
CAGAGTA15800.028.37466210
CGCAGAG15950.028.1014468
GCAGAGT15850.028.0644669
GTCCACT12800.027.8677367
Produced by FastQC (version 0.11.5)