FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718581
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23150.32216270677905484No Hit
GATTATTACTGTTGCTCATATGCAGGCAGCCACATTTTCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG20260.28194455461527645No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18570.2584259812046241No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC18550.25814765489207203No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAAT16410.2283667394489974No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15690.2183469921971218No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15550.21639870800925715No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15510.21584205538415294No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15450.2150070764464966No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG14940.2079097554764181No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGTTGCTCATAT14910.20749226600758996No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT14660.2040131871006887No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14430.2008124345063396No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA13790.19190599250467238No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAACAGTAATAATCAG13580.18898356622287538No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13290.18494783469086992No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13080.1820254084090729No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12990.18077294000258842No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT12800.17812884003334348No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12790.17798967687706746No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12600.17534557690782251No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12540.17451059797016621No Hit
GTCTTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA12340.1717273348446452No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC12330.17158817168836915No Hit
GTCTCTGGTGGCTCCATCAACAGGAGTGGTTACTCCTGGGGCTGGATCCG12060.16783076646891582No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG11940.16616080859360324No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11910.16574331912477508No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGA11890.16546499281222296No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG11580.16115093496766544No Hit
GGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11340.15781101921704024No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11260.15669771396683185No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11110.15461026662269112No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11080.15419277715386295No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10980.15280114559110244No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA10740.14946122984047727No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10730.14932206668420123No Hit
CCCCTGAGGGCCGCTCATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA10690.14876541405909702No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10570.14709545618378442No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10430.14514717199591973No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10400.1447296825270916No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10120.1408331141513622No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10040.1397198089011538No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATGAG9960.1386065036509454No Hit
CTATAAGACATACTCCCTATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCAGGAGTAACCACTC9890.13763236155701306No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGC9830.13679738261935676No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9800.13637989315052862No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC9630.13401411949383576No Hit
CCTGAGGGCCGCTCATTGACATCATAAATCATGAGTTTGGGGGCTTTGCC9340.1299783879618303No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9310.12956089849300217No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9270.129004245867898No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9130.1270559616800333No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9030.1256643301172728No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8970.12482935117961648No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG8890.1237160459294081No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8860.12329855646057994No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8830.1228810669917518No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT8670.120654456491335No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8540.11884533545974638No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8430.11731454074070981No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8230.11453127761518883No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8170.11369629867753253No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA8120.11300048289615226No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8060.11216550395849598No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8010.11146968817711572No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7950.11063470923945942No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7920.11021721977063129No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGTTGCTCATATGCAGGCAGCCACATTTTCTT7820.10882558820787078No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7800.1085472618953187No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7740.1077122829576624No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7650.10645981455117794No Hit
GTATTACTGTGGGAGACACATTTATGGTTCATACTACTACTACTACTACA7620.1060423250823498No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT7560.10520734614469351No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAACAGGAGTGGTTACTCCTGGG7560.10520734614469351No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7450.10367655142565695No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7430.10339822511310486No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7410.10311989880055275No Hit
GCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAACAGTAATAATCAGCCTCATCTTCAGC7310.10172826723779227No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7290.10144994092524016No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7240.10075412514385991No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7240.10075412514385991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)