FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718581
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41460.5769704459205016No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20730.2884852229602508No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19180.2669149337374631No Hit
GATTATTACTGTTGCTCATATGCAGGCAGCCACATTTTCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG19000.26440999692449424No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC16130.22447017107326803No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAAT15640.21765117641574155No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15240.2120846501646996No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG14560.2026215555379282No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14490.20164741344399587No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14090.1960808871929539No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14080.19594172403667784No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13940.19399343984881315No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13650.18995770831680772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13640.18981854516053168No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA13010.18105126631514054No Hit
GTCTTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA12990.18077294000258842No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGTTGCTCATAT12750.17743302425196325No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12700.176737208470583No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAACAGTAATAATCAG12630.17576306637665065No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC12000.16699578753125954No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT11810.1643516875620146No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11610.16156842443649358No Hit
GTCTCTGGTGGCTCCATCAACAGGAGTGGTTACTCCTGGGGCTGGATCCG11200.15586273502917555No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11070.1540536139975869No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11050.15377528768503482No Hit
CCCCTGAGGGCCGCTCATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA10960.15252281927855038No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10840.15085286140323778No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10810.15043537193440962No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10760.14973955615302936No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG10630.14793043512144072No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG10600.14751294565261258No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10570.14709545618378442No Hit
GGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC10200.1419464194015706No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10180.1416680930890185No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGA10030.13958064574487775No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9860.13721487208818492No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGC9830.13679738261935676No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9810.13651905630680466No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9580.1333183037124555No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA9540.13276165108735133No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9400.1308133668994866No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9240.12858675639906983No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9180.12775177746141353No Hit
CTATAAGACATACTCCCTATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCAGGAGTAACCACTC9010.1253860038047207No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8860.12329855646057994No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATGAG8780.12218525121037156No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8750.1217677617415434No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8730.1214894354289913No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8460.11773203020953797No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC8450.11759286705326191No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8450.11759286705326191No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8360.11634039864677748No Hit
CCTGAGGGCCGCTCATTGACATCATAAATCATGAGTTTGGGGGCTTTGCC8360.11634039864677748No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8130.11313964605242832No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8030.11174801448966783No Hit
GCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAACAGTAATAATCAGCCTCATCTTCAGC8020.11160885133339178No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7870.10952140398925105No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG7740.1077122829576624No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT7640.10632065139490189No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7610.10590316192607376No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA7600.1057639987697977No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7520.10465069351958929No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7520.10465069351958929No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7390.10284157248800065No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAACAGGAGTGGTTACTCCTGGG7320.10186743039406831No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGTTGCTCATATGCAGGCAGCCACATTTTCTT7270.10117161461268805No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7260.10103245145641201No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7230.10061496198758386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT150.002527854967.779676
TACGTAT400.005816066654.91361670
GGTATCA7900.040.8018841
GTATCAA18300.036.3403171
GTGGTAT2450.031.8526441
TATCAAC21450.030.988512
TATTAGA450.00502585130.1452872
AATAGCG604.1536512E-430.0867462
CAAACGA703.4899844E-529.99861149
GAATGGG1000.00578335429.28726470
TCAACGC22250.029.2462984
ATCAACG22400.029.203793
TCTACCG6300.029.05482170
CAACGCA22450.028.8327795
ATCGTTG605.4653484E-428.70919240
AACGCAG22700.028.664536
TAATCGT605.5979344E-428.59183938
TACTCCG606.0166063E-428.2415315
GACAGGT1452.7284841E-1128.0799921
TGGTATC2800.027.8574712
Produced by FastQC (version 0.11.5)