FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4069
Sequences flagged as poor quality0
Sequence length22-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT68416.810027033669208No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC1894.644875890882281No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG661.62202015237159No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC340.8355861391005162No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGGCGT320.7864340132710739No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGG260.6389776357827476No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGT250.6144015728680265No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCCGT180.4423691324649791No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATGCCGT140.3440648808060949No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCT130.3194888178913738No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATTCCGT100.2457606291472106No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCC90.22118456623248955No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATTCC80.19660850331776847No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATGCC80.19660850331776847No Hit
GATTACTTCTGTGCATCTTGGCATGACAGCCGGAACGCCGATTATGTCTTCGGCACTGGGACCACGGTCACCGTC70.17203244040304744No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCG70.17203244040304744No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC60.14745637748832638No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATGTCGTATGCCGT60.14745637748832638No Hit
CTGGTAGCGCTAGCAAATCCTCCAATGA50.1228803145736053No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGGC50.1228803145736053No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTGGTATGCCGT50.1228803145736053No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATTCCG50.1228803145736053No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG50.1228803145736053No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTTTGCCGG50.1228803145736053No Hit
CACTGGTAGCGCTAGCAAATCCTCCAATGA50.1228803145736053No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT50.1228803145736053No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATGTCGTATGCC50.1228803145736053No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCC50.1228803145736053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG650.0117.1111170
TGGGGGG152.5160916E-4117.1111170
GTGAAAA150.001530853875.2857158
GGTGTAA150.001530853875.2857157
AAGGGGG700.074.0515269
TGCTTGA700.064.5306255
CTGCTTG700.064.5306254
GCTTGAA650.063.70329756
CTTGAAA650.063.70329757
CTTCTGC700.062.738151
TTCTGCT650.061.93375852
AAAGGGG800.061.75781268
AAAAGGG800.059.88636467
GAAAAAA950.059.43609260
TGAATCT1050.059.43609234
TGAAAAA950.059.43609259
AATCTCG1000.058.9407936
ATCTCGT1000.058.9407937
GAATCTC1000.058.9407935
TCGTATG1000.058.9407940
Produced by FastQC (version 0.11.5)