FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026854
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41770.40677642585995666No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29780.29001201728775466No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27350.26634750412424746No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26780.2607965689377458No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA25320.24657838407407479No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24530.2388849826752391No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23350.2273935729909023No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22020.21444139088906505No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21220.206650604662396No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21020.20470290810572878No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20880.20333952051606166No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18770.1827913218432221No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC18190.17714300182888706No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAACCAGTGCCCCAGGTCTGACAGTAATAGTCAG17900.17431884182171953No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17430.1697417549135515No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17380.1692548307743847No Hit
GACTATTACTGTCAGACCTGGGGCACTGGTTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG17080.1663332859393838No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16780.1634117411043829No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16610.16175619903121574No Hit
GCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT16120.15698434246738097No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16030.1561078790168807No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15780.1536732583210466No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15380.1497778652077121No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15120.14724585968404466No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14930.14539554795521076No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14900.1451033934717107No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14520.1414027700140429No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14420.14042892173570926No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14390.14013676725220917No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14300.13926030380170892No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG14140.13770214655637508No Hit
CTATTACTGTCAGACCTGGGGCACTGGTTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC13910.13546229551620775No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGC13880.13517014103270766No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC13700.13341721413170712No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13560.13205382654204006No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13330.1298139755018727No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT12950.12611335204420493No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12920.1258211975607048No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12910.12572381273287148No Hit
GGTTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT12820.1248473492823712No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12410.12085457134120334No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG12280.11958856857936961No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT12000.11686179340003545No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC11940.11627748443303527No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11860.11549840581036837No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11760.11452455753203473No Hit
GCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC11740.11432978787636801No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT11720.1141350182207013No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC11550.11247947614753412No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11460.11160301269703386No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11340.11043439476303349No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGTTTTTGGGTGTTCGGCGGAGGGACCAAG11260.1096553161403666No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11240.10946054648469988No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG11070.10780500441153269No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11050.10761023475586598No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10950.10663638647753235No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAG10850.10566253819919871No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC10850.10566253819919871No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT10800.1051756140600319No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10660.10381222647036481No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10600.10322791750336466No Hit
CTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACT10500.10225406922503102No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10370.1009880664631973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAC200.007268248651.912243
TGCGCAA200.00832801550.15533410
GTGGTAT5050.043.096341
TGGTATC5450.040.5121082
CCTAACG504.748481E-640.1438142
GGTATCA15800.039.2042431
CTAACGC451.2027316E-437.1593363
TCTGGAG4100.035.57387570
GTATCAA36150.035.381281
ATCATAC450.00376391731.98136955
TATCAAC41350.030.9856552
ATCAACG41600.030.4688743
TCAACGC42150.029.9890294
GTAGAAC450.005381833829.727473
CAACGCA42800.029.5335885
AACGCAG43500.029.135216
CGCAGAG46700.027.3536118
CGGTAAC550.0080804527.35329665
GCTTGGG1603.6379788E-1227.2045671
AGAGTAC48100.026.692611
Produced by FastQC (version 0.11.5)