FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883112
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA76570.8670474413211461No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60880.6893802824556794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50310.5696899147559992No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA46620.5279058601853446No Hit
ATATTATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT41830.4736658543876654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39370.445809818007229No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38550.43652447254708354No Hit
TTATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT36770.4163684787433531No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36020.4078757847249273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35620.4033463479151002No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC32900.3725461776082762No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32240.3650726068720615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31760.359637282700269No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31650.35839168757756656No Hit
CTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGGATCCGGGGTCCCT30930.3502387013198779No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29050.3289503483136907No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT29040.328837112393445No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28760.32566650662656604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27460.3109458369946281No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27240.3084546467492232No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26790.30335903033816775No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26560.3007546041725172No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25830.29248838199458277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25280.2862604063810706No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT25220.28558099085959654No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24750.2802589026080497No Hit
AACCAGGACAGCCTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGGA24470.27708829684117076No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC24420.27652211723994236No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24140.27335151147306347No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA23660.267916187301271No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22610.2560264156754749No Hit
CCTCATTTACTGGGCATCTGCCCGGGGATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT22560.25546023607424656No Hit
GTCAGGGACCCCGGATCCCCGGGCAGATGCCCAGTAAATGAGGAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA21940.24843960901901455No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21850.24742048573680347No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21360.2418719256447653No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA20580.23303952386560253No Hit
GTAAATGAGGAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA20540.23258658018461983No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20050.2270380200925817No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19980.22624536865086198No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19660.22262181920300028No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC19210.21752620279194482No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19210.21752620279194482No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC19170.21707325911096217No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18280.20699526220909695No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18210.2062026107673772No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17860.2022393535587785No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAG17760.20110699435632173No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG17570.1989555118716539No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17340.19635108570600332No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA16640.18842457128880594No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16620.1881980994483146No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16150.1828760111967678No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT16090.18219659567529373No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15990.18106423647283695No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15570.17630832782251854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15540.17596862006178152No Hit
CACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGC15460.1750627326998161No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15400.17438331717834205No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15370.17404360941760502No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15280.17302448613539392No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14780.16736269012311009No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14640.16577738723967061No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14540.16464502803721384No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14400.1630597251537744No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13930.15773763690222758No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13900.15739792914149056No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13810.15637880585927946No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGGATC13770.15592586217829676No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13760.15581262625805106No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13700.15513321073657702No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13410.1518493690494524No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTCCTCATT13340.15105671760773265No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13300.15060377392674995No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13190.1493581788040475No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13180.14924494288380183No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12920.14630080895741424No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12840.1453949215954488No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12790.14482874199422044No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12490.14143166438685015No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12480.14131842846660447No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA12370.14007283334390203No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12000.13588310429481198No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11990.1357698683745663No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATG11930.13509045285309224No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11920.13497721693284656No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11900.1347507450923552No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11770.13327867812916142No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11760.13316544220891574No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG11750.13305220628867007No Hit
ATTATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11750.13305220628867007No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11560.13090072380400222No Hit
CCACTGAATCGGTCAGGGACCCCGGATCCCCGGGCAGATGCCCAGTAAAT11530.13056101604326517No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11370.12874924131933435No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11260.1275036461966319No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11190.12671099475491218No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11130.1260315792334381No Hit
GCCCGGGGATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC11070.12535216371196406No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10960.12410656858926161No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTCCTCATTTACTGGGCATCTGC10750.12172861426410239No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10700.12116243466287402No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAA10650.12059625506164563No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10460.11844477257697777No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10290.11651976193280127No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA10270.11629329009230992No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10230.11584034641132722No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT10230.11584034641132722No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATCCCCGGGC10120.11459475128862479No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGGATCCGGGGTCCCTGA10100.11436827944813342No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10070.11402857168739639No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10030.11357562800641367No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGGATCCGGGGTC9970.11289621248493963No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9940.11255650472420259No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTCC9940.11255650472420259No Hit
GGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGAT9910.11221679696346556No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9850.1115373814419915No Hit
TTATTACTGTCAGCAATATTATAGTACTCCTCCGACTTTTGGCCAGGGGA9840.11142414552174583No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC9830.11131090960150014No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9790.11085796592051743No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9770.1106314940800261No Hit
GCAGAAACCAGGACAGCCTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGGATCCGGGGTCCCTGACCGATT9770.1106314940800261No Hit
CATTTACTGGGCATCTGCCCGGGGATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA9730.1101785503990434No Hit
CCCCGGATCCCCGGGCAGATGCCCAGTAAATGAGGAGCTTAGGAGGCTGT9580.10848001159535824No Hit
GCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACTCTGGCTGGACTT9580.10848001159535824No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG9530.10791383199412986No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCGGAGG9460.1071211805524101No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT9350.10587558542970767No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9310.10542264174872495No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG9310.10542264174872495No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG9210.1042902825462682No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9130.10338439518430279No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC9120.10327115926405711No Hit
TCCCCGGGCAGATGCCCAGTAAATGAGGAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT8990.10179909230086331No Hit
GTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACT8920.10100644085914358No Hit
ATAGTACTCCTCCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA8880.10055349717816087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCGGAGGAGTA8870.10044026125791518No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8850.10021378941742383No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG8850.10021378941742383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCG200.006322128753.77475458
GTGGTAT1350.052.5893251
GTATAGA200.007972673550.7111361
ATCTCGT1350.048.2087537
TCGTATG1450.047.5738940
GACAGGT800.046.4852071
ATGCCGT1500.046.2930144
CCGTCTT1550.045.03278447
CGTCTTC1600.043.74058548
ACGAGAC1550.043.63236622
CGTATGC1600.043.17620541
AGACGGA1600.042.31439225
GCCGTCT1650.042.22699446
TATGCCG1650.042.0161343
CATCTCG1550.041.9161136
AATGGTA2450.041.7801570
GCCCACG1650.040.97637618
TCCGAGC1650.040.97173313
ATCTCCG1650.040.96941410
TATACAC1750.040.568913
Produced by FastQC (version 0.11.5)