FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401416
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55430.395528522580019No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATCTTGTCAAAGCCTCTATGCGAGGTG54200.3867516854381568No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATCTTGTCAAAGCCTCTATGCGAGGTGCAGTAATAGT51750.3692693675539597No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG49110.3504312780787432No Hit
GTCTATAGGCGGCCCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT46870.3344474445846201No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46710.3333057421921828No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG42520.3034074107902293No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACTAGTAGTGACGTT40180.2867100133008329No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39640.28285676772635676No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC37670.2687995570194717No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36730.26209205546390224No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35530.25352928752062204No Hit
CCTATAGACCTCATAAATGATGAGTTTGGGGGCCTTGCCTGATTTCTGTT35190.25110316993669257No Hit
TTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC34820.2484629831541812No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34590.24682178596505247No Hit
GTGCAGTAATAGTCACCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG34570.24667907316599783No Hit
GTAATAAATACCCGTGTCACCGGCTCTCAGGTTGTTCATTTGCAGATACA33270.23740274122744423No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32330.23069523967187475No Hit
CTCCTGCACTGGAACTAGTAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGAAATCAGGCAAGGC29220.20850339941887347No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGGTGACTATTACTGCACCTCGCAT27870.19887028548268323No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27850.19872757268362856No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27500.19623009870017183No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG27280.19466025791057046No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26770.19102108153467634No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC26610.18987937914223899No Hit
ATTTATTACTGTGCGACTATAGAGGCGTTTCGGTTTGACTACTGGGGCCA26610.18987937914223899No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26280.18752461795783693No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26090.18616884636681758No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25870.1845990055772162No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25750.18374272878288817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25180.17967541400983006No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGGTATTTTAGAAGG25170.17960405761030274No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25030.17860506801692003No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC24290.17332469445189722No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24270.17318198165284254No Hit
GTCAGTCACCATCTCCTGCACTGGAACTAGTAGTGACGTTGGTGGTTATA24060.17168349726276852No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23920.17068450766938584No Hit
ATATAAACCCAGATTCACAAAAAAAGATTTTAGTGGAGTCAGTGGAGGGCCGATTCACCGTCTCCAGAGACAACG23820.16997094367411245No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23530.16790160808781976No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23120.164975995707199No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT22960.16383429331476163No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22700.1619790269270509No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22520.1606946117355589No Hit
CCCCTGAGGGCCGCCTATAGACCTCATAAATGATGAGTTTGGGGGCCTTGCCTGATTTCTGTTGGTACCAGGAGA22470.1603378297379222No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22450.16019511693886754No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21970.15677000976155545No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT21250.1516323489955873No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATCTTGTCAAAGC20960.1495630134092946No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20570.14678011382772851No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20440.14585248063387318No Hit
CTATTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAG20410.14563841143529116No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20390.1454956986362365No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG20160.1438545014471078No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20080.1432836502508891No Hit
GGATGAGGGTGACTATTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAGGGACCAAGCTGAC20060.14314093745183443No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20030.14292686825325243No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20020.14285551185372508No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19930.14221330425797907No Hit
CCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG19460.13885955348019433No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19210.13707564349201093No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAACCGATCAGGGACCCCTGAGGGCCGC19030.1357912283005189No Hit
GACTATTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18840.13443545670949955No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18760.13386460551328083No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18320.1307249239340781No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG18290.1305108547354961No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18130.12936915234305874No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC18010.12851287554873073No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC17910.12779931155345736No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA17890.1276565987544027No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17760.12672896556054733No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT17760.12672896556054733No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17730.12651489636196533No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17320.1235892839813446No Hit
GTGGAGGGCCGATTCACCGTCTCCAGAGACAACGCCAGGAATTCCCTGTA17240.1230184327851259No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17160.12244758158890723No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17060.12173401759363388No Hit
GTAATAGTCACCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG16950.12094909719883318No Hit
CCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC16880.12044960240214184No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16790.11980739480639581No Hit
CTCCAGAGACAACGCCAGGAATTCCCTGTATCTGCAAATGAACAACCTGAGAGCCGGTGACACGGGTATTTATTA16760.1195933256078138No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC16180.11545465443522837No Hit
GCCTTAGCCAAGGCCTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC16170.11538329803570102No Hit
GTTTGGGGGCCTTGCCTGATTTCTGTTGGTACCAGGAGACAAAGTTATAA16150.11524058523664636No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16070.11466973404042767No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT16050.11452702124137301No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15960.113884813645627No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGAAATCAGGCAAGGCCCCCAAACTCATCATTTATGAGGTCTATAGG15930.11367074444704499No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAACCGATCAGGGAC15900.11345667524846298No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC15620.1114586960616976No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15540.11088784486547891No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15460.11031699366926023No Hit
CAGTAATAGTCACCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGA15320.10931800407587755No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15240.10874715287965886No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15190.10839037088202219No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15020.10717731209005747No Hit
GTCTCAGAGAGGAGCCTTAGCCAAGGCCTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA14900.10632103529572946No Hit
ATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG14830.10582154049903812No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC14620.10432305610896407No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG14590.10410898691038206No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA14490.10339542291510871No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGGTGACTATTACT14450.10310999731699938No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14420.10289592811841737No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT14280.10189693852503467No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14220.10146880012787067No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA14210.10139744372834333No Hit
GGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACG14170.101112018130234No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14040.10018438493637864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG204.8318726E-4102.8171870
GGTATCA7550.048.11411
TCTCGTA551.4783473E-743.8777538
CGCTAAT551.5201476E-743.7223832
GTGGTAT1750.043.082221
ACGAGAC651.2072633E-842.18140422
AATCTCG602.952347E-740.17800536
GTATCAA18950.038.1580921
TCGTATG655.407819E-737.1927440
TCGCTAA655.6579665E-736.97866431
TGGTATC2050.036.77752
AATGGTA4900.034.9718370
TAACCGT501.7196852E-434.9686753
CACGAGA807.521339E-834.27239221
TATCAAC22200.032.4175152
CGTATGC751.6467056E-632.25691241
ATAACCA14500.032.1451470
TTAACGG553.300763E-431.29295335
ATCAACG23200.030.8724943
GTATACA450.00477682730.4621754
Produced by FastQC (version 0.11.5)