FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401416
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51470.3672713883671943No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATCTTGTCAAAGCCTCTATGCGAGGTG50610.36113473800784346No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG49620.3540704544546373No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATCTTGTCAAAGCCTCTATGCGAGGTGCAGTAATAGT49060.3500744960811065No Hit
GTCTATAGGCGGCCCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT44980.32096108507395377No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACTAGTAGTGACGTT42230.30133807520393663No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40700.29042054607625434No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39400.28114421413770074No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35680.254599633513532No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC35640.2543142079154227No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33760.24089920480428367No Hit
GTGCAGTAATAGTCACCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG33540.2393293640146823No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32420.23133744726762076No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32160.22948218087991004No Hit
TTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC32130.22926811168132805No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31910.22769827089172665No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30970.22099076933615713No Hit
CCTATAGACCTCATAAATGATGAGTTTGGGGGCCTTGCCTGATTTCTGTT30870.2202772053408838No Hit
CTCCTGCACTGGAACTAGTAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGAAATCAGGCAAGGC29480.2103586658065842No Hit
GTAATAAATACCCGTGTCACCGGCTCTCAGGTTGTTCATTTGCAGATACA29440.21007324020847484No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC28640.20436472824628804No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26430.18859496395074696No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGGTGACTATTACTGCACCTCGCAT26310.18773868715641892No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25910.18488443117532552No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25880.18467036197674352No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25500.1819588187947048No Hit
ATTTATTACTGTGCGACTATAGAGGCGTTTCGGTTTGACTACTGGGGCCA25290.18046033440463075No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25100.17910456281361137No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25010.17846235521786535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24980.17824828601928336No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24130.17218299205945986No Hit
GTCAGTCACCATCTCCTGCACTGGAACTAGTAGTGACGTTGGTGGTTATA23910.1706131512698585No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA23890.17047043847080381No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23550.1680443208868744No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC23520.1678302516882924No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23360.16668854929585503No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23320.1664031236977457No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT23100.16483328290814434No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22760.16240716532421492No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG22210.1584825633502115No Hit
ATATAAACCCAGATTCACAAAAAAAGATTTTAGTGGAGTCAGTGGAGGGCCGATTCACCGTCTCCAGAGACAACG21800.15555695096959077No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21320.15213184379227865No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21170.15106149779936864No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGGTATTTTAGAAGG21130.1507760722012593No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21010.1499197954069313No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21000.14984843900740394No Hit
CCCCTGAGGGCCGCCTATAGACCTCATAAATGATGAGTTTGGGGGCCTTGCCTGATTTCTGTTGGTACCAGGAGA20890.14906351861260325No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20810.1484926674163846No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT20330.14506756023907247No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20320.14499620383954515No Hit
GGATGAGGGTGACTATTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAGGGACCAAGCTGAC20090.14335500665041645No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19960.1424273734565611No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG19500.13914497907830367No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG19360.13814598948492096No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19330.13793192028633897No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19220.13714699989153825No Hit
CTATTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAG19130.13650479229579227No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18460.1317239135274608No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18160.12958322154164073No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC18060.1288696575463674No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18050.12879830114684007No Hit
GACTATTACTGCACCTCGCATAGAGGCTTTGACAAGATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT17910.12779931155345736No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATCTTGTCAAAGC17850.12737117315629334No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17710.12637218356291063No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC17480.12473098637378194No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17370.12394606597898125No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17220.12287571998607122No Hit
GTGGAGGGCCGATTCACCGTCTCCAGAGACAACGCCAGGAATTCCCTGTA16980.12116316639741519No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAACCGATCAGGGACCCCTGAGGGCCGC16970.12109180999788785No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16940.12087774079930584No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16820.12002146400497782No Hit
GTTTGGGGGCCTTGCCTGATTTCTGTTGGTACCAGGAGACAAAGTTATAA16610.11852297961490378No Hit
CCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG16580.11830891041632177No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16270.1160968620309744No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA16210.11566872363381038No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG16100.11488380323900968No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15980.11402752644468167No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGAAATCAGGCAAGGCCCCCAAACTCATCATTTATGAGGTCTATAGG15940.11374210084657231No Hit
CTCCAGAGACAACGCCAGGAATTCCCTGTATCTGCAAATGAACAACCTGAGAGCCGGTGACACGGGTATTTATTA15640.11160140886075226No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC15420.1100315680711509No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15350.10953207327445956No Hit
CCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC15350.10953207327445956No Hit
GGGTTTATATTGGCCACCCACTCAAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATGGCTGCTGAAA15100.10774816328627616No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15000.10703459929100281No Hit
GCCTTAGCCAAGGCCTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14960.10674917369289348No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAACCGATCAGGGAC14800.10560747130045611No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14790.10553611490092876No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT14760.10532204570234678No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14740.1051793329032921No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14730.10510797650376477No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14730.10510797650376477No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14710.1049652637047101No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA14600.10418034330990941No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14580.10403763051085474No Hit
GTAATAGTCACCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG14420.10289592811841737No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14320.10218236412314402No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14270.10182558212550735No Hit
GGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACG14150.10096930533117932No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA14110.10068387973306998No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14100.10061252333354263No Hit
GTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGC14100.10061252333354263No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC14040.10018438493637864No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG14020.10004167213732397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT204.6967284E-4103.5531370
GCTACGG150.002335460969.1499341
GTACGTA302.2243749E-456.82478769
CTATCGA200.00758033151.3642169
GTGGTAT2350.045.1423531
GGTACGT358.579741E-443.22036768
GGTATCA7250.041.5369871
AATGGTA4350.039.6755370
TGGTATC2600.039.479922
GTATCAA17600.037.5262341
TAACGGC400.002614318234.45197736
TATCAAC20150.032.9423872
TCTGGAG15500.032.0680770
ATCAACG20800.031.4171923
ATAACCA15450.030.83135670
TCAACGC21300.030.6797034
TATACTG450.004811302330.4174165
CAACGCA21600.030.2589915
TGCATAG1154.2119448E-430.015470
ATTAGAG802.9477087E-629.93683
Produced by FastQC (version 0.11.5)