FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467353
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGCCTGAGTAGTTGGGTGTTCGG28280.19272799387741055No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27840.18972939708440983No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG26430.18012025736138476No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA24940.16996591822145046No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21360.1455682443147627No Hit
AGTCTATACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG21230.1446822952622852No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19930.13582280473751035No Hit
GTCCTGGGCCCAGTCTATACTGACTCAGCCACCCTCAGCGTCTGGGACCC19150.1305071104226454No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAACTACTCAGG18150.1236921177112801No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC18130.1235558178570528No Hit
GTCTATACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG17710.12069352091827938No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16260.1108117814867997No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16180.11026658206989047No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15250.10392863884832075No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15110.10297453986872962No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15060.10263379023316135No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14870.10133894161800194No Hit
GTGCAGGGTCCTGGGCCCAGTCTATACTGACTCAGCCACCCTCAGCGTCT14830.10106634190954733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)