FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004687362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467353
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGCCTGAGTAGTTGGGTGTTCGG25730.17534976246342904No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24650.16798957033515452No Hit
AGTCTATACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG22370.15245138695324165No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA20740.14134294883371623No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20520.13984365043721586No Hit
GTCCTGGGCCCAGTCTATACTGACTCAGCCACCCTCAGCGTCTGGGACCC20230.13786730255091995No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18640.1270314641398491No Hit
GTCTATACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG18270.12450991683664395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17500.11926237244889266No Hit
GTGCAGGGTCCTGGGCCCAGTCTATACTGACTCAGCCACCCTCAGCGTCT15640.10658648600575323No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC15570.10610943651595764No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAACTACTCAGG14680.10004409300284253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)